Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   AMM49_RS01170 Genome accession   NZ_CP012480
Coordinates   196572..197615 (+) Length   347 a.a.
NCBI ID   WP_223297804.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain NGBS128     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 191572..202615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMM49_RS01160 (AMM49_01155) - 195170..195535 (+) 366 WP_000285373.1 DUF1033 family protein -
  AMM49_RS10915 (AMM49_01160) comGA 195707..196678 (+) 972 Protein_164 competence type IV pilus ATPase ComGA -
  AMM49_RS01170 (AMM49_01165) comYB 196572..197615 (+) 1044 WP_223297804.1 competence type IV pilus assembly protein ComGB Machinery gene
  AMM49_RS01175 (AMM49_01170) comGF 197640..198068 (+) 429 WP_223842274.1 competence type IV pilus minor pilin ComGF -
  AMM49_RS01180 (AMM49_01175) comGG 198046..198269 (+) 224 Protein_167 competence type IV pilus minor pilin ComGG -
  AMM49_RS01185 (AMM49_01180) comYH 198384..199358 (+) 975 WP_001008572.1 class I SAM-dependent methyltransferase Machinery gene
  AMM49_RS01190 (AMM49_01185) - 199390..200583 (+) 1194 WP_000047534.1 acetate kinase -
  AMM49_RS01195 (AMM49_01190) - 200734..200940 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  AMM49_RS11200 - 200999..201136 (+) 138 WP_001869149.1 hypothetical protein -
  AMM49_RS01200 (AMM49_01195) - 201177..201632 (+) 456 WP_000905672.1 hypothetical protein -
  AMM49_RS01205 (AMM49_01200) - 201701..202366 (+) 666 WP_000008107.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 40014.77 Da        Isoelectric Point: 9.9642

>NTDB_id=154580 AMM49_RS01170 WP_223297804.1 196572..197615(+) (comYB) [Streptococcus agalactiae strain NGBS128]
MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDRMNKALLEGKDLSKML
GELGFSDTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGLRHYLMPQLGENNFATR
LITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTIPLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQN
QKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVI
FIFVALIIVMIYAAMLLPMYQNMEILS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=154580 AMM49_RS01170 WP_223297804.1 196572..197615(+) (comYB) [Streptococcus agalactiae strain NGBS128]
ATAGACAAGTGGATATCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAA
ACAACGGAAAGTAGTCCAACTTTTTAATAATCTTTTTGCTAGTGGTTTTTCTTTAACTGATATGGTCACCTTCTTAAAGA
GGAGTAAGTTATTGTCTGATTGTTATACAGATAGGATGAATAAGGCATTATTAGAGGGAAAAGATTTATCAAAAATGTTA
GGAGAGTTAGGTTTTTCTGACACTGTTATCACACAGGTTGCATTAGCTGATTTGCATGGTAACATTTCAAGGAGCCTACT
AAAGATTGAGTCTTATTTAGCTAATCTTTTGTTAGTTAGAAAAAAAGTAATAGAAGTAGCTACTTACCCATTGATATTAT
TGTCTTTTCTGGTGCTAATTATGATTGGCCTTAGGCATTATTTAATGCCCCAATTAGGAGAAAATAATTTTGCAACTAGA
CTGATTACAAATGTGCCGAATATTTTCTTATTACTTTTAGCAGTTGTACTTATTTTTAGTTTAATATTTTATATTATTCA
AAAGCGATTGTCGCGCATTAAAGTAGCTTGTTTTTTAACAACAATTCCCTTAGTTGGATCATATGTTAAGCTTTATTTAA
CTGCTTACTATGCCCGTGAGTGGGGAAATTTATTAAGTCAAGGTGTTGAATTGGACCAAATTGTTAAAGTAATGCAAAAT
CAGAAATCCAAACTTTTTAGGGAAATAGGATATGACATGGAAGAAGGTTTTCTATCAGGTAAAGCATTTCACCAAAAAGT
ATTAGACTATCCGTTTTTCTTAACTGAGCTTAGTTTAATGATTGAATATGGTCAAGTTAAGGCGAAATTAGGAACAGAGT
TAGATATATATGCTGATGAGAAGTGGGAGGATTTTTTTACAAAATTAGCTAGAGCGACCCAATTAATCCAACCCGTTATT
TTTATTTTTGTAGCTCTTATCATTGTTATGATTTATGCAGCAATGCTGTTACCAATGTATCAAAATATGGAGATATTATC
ATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)

Type II secretion system (T2SS), protein F (T2SSF)

(33-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

62.17

98.271

0.611
  comYB Streptococcus mutans UA159

61.877

98.271

0.608
  comYB Streptococcus gordonii str. Challis substr. CH1

56.012

98.271

0.55
  comGB/cglB Streptococcus mitis NCTC 12261

54.925

96.542

0.53
  comGB/cglB Streptococcus mitis SK321

54.328

96.542

0.524
  comGB/cglB Streptococcus pneumoniae Rx1

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae D39

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae R6

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae TIGR4

53.433

96.542

0.516
  comGB Lactococcus lactis subsp. cremoris KW2

50.148

97.118

0.487

Multiple sequence alignment