Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   A7319_RS18940 Genome accession   NZ_CP015638
Coordinates   4311733..4313463 (+) Length   576 a.a.
NCBI ID   WP_024076414.1    Uniprot ID   A0ACA7PA05
Organism   Pseudomonas fluorescens strain L111     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4306733..4318463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7319_RS18930 (A7319_18915) - 4308094..4310844 (-) 2751 WP_069076598.1 beta-glucosidase -
  A7319_RS18935 (A7319_18920) - 4311352..4311576 (+) 225 WP_041160973.1 hypothetical protein -
  A7319_RS18940 (A7319_18925) pilF 4311733..4313463 (+) 1731 WP_024076414.1 type II secretion system ATPase GspE Machinery gene
  A7319_RS18945 (A7319_18930) gspF 4313460..4314662 (+) 1203 WP_069076599.1 type II secretion system inner membrane protein GspF -
  A7319_RS18950 (A7319_18935) gspG 4314683..4315108 (+) 426 WP_069076600.1 type II secretion system major pseudopilin GspG -
  A7319_RS18955 (A7319_18940) - 4315118..4315549 (+) 432 WP_069076601.1 type II secretion system protein -
  A7319_RS18960 (A7319_18945) - 4315546..4315914 (+) 369 WP_069076602.1 type II secretion system protein -
  A7319_RS18965 (A7319_18950) - 4315911..4316540 (+) 630 WP_069076603.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  A7319_RS18970 (A7319_18955) - 4316537..4317262 (+) 726 WP_069076604.1 general secretion pathway protein GspK -
  A7319_RS18975 (A7319_18960) - 4317259..4318302 (+) 1044 WP_069076605.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 63311.65 Da        Isoelectric Point: 5.5098

>NTDB_id=154356 A7319_RS18940 WP_024076414.1 4311733..4313463(+) (pilF) [Pseudomonas fluorescens strain L111]
MPSSSTPIDACQLHIPHTEQVCAWLMQHAGLKTVDLERARRLSQEGGDAELLGLLTRLGLVSEVELARAWADLLGAPLLL
ADAAPPLLDPLPVLTERFMHHYQVVPVGWSQGGLRVLAANPSLVYPFQAIAYACGVPVWLAVGPRNEVETLIERYYGQGR
SAMGTLIENLDEQGSALEDIEHLKDMASEAPVIRLVNLILQRAVEHRASDIHIEPFENQLKVRYRIDGVLHEAEAPPSSS
SAAVISRVKIMARLDIAERRLPQDGRIMLRIQGKELDLRVSTVPTSFGESVVMRLLDRQTVQFDFQSLGFDGQRLETFLE
VLERPHGILLVTGPTGSGKTTTLYTALSRLNTAERKIITVEDPVEYQLEGINQIQVKPAIGLDFAGALRSIVRQDPDVIM
IGEIRDLETCRIAIQSSLTGHLVLSTLHTNSAAASITRLLDMGVESYLIASTVNGILAQRLVRRLDPATREAFEAPAELI
AEHGLDRFTEQRPILLYRPRADAPGGGYHGRSAITELLVMNDELRSLLMRQADAATLEQAARRGGLRTLHEEGLRQAVAG
VTSLEEVLRVTRGEGA

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=154356 A7319_RS18940 WP_024076414.1 4311733..4313463(+) (pilF) [Pseudomonas fluorescens strain L111]
ATGCCCAGTTCCTCAACGCCCATCGATGCCTGCCAACTGCACATCCCGCACACCGAACAGGTCTGCGCGTGGCTGATGCA
ACACGCTGGGTTGAAAACCGTTGACCTGGAGCGCGCCCGGCGCCTGTCCCAGGAGGGCGGCGATGCCGAGTTGCTCGGCC
TGCTCACACGTTTAGGGCTGGTCTCGGAAGTGGAACTGGCCCGTGCCTGGGCGGACCTGCTCGGCGCACCGCTGCTGCTG
GCCGATGCGGCGCCGCCGCTGCTTGATCCGTTGCCGGTATTGACCGAACGCTTCATGCACCACTACCAGGTGGTGCCCGT
GGGCTGGAGCCAGGGCGGCCTGCGGGTACTGGCGGCCAACCCGTCGCTGGTCTACCCGTTCCAGGCCATCGCCTATGCCT
GCGGCGTACCCGTGTGGCTGGCCGTGGGCCCGCGCAACGAAGTCGAAACCCTGATCGAGCGCTACTACGGCCAGGGCCGT
TCGGCCATGGGCACCCTGATCGAAAACCTCGACGAGCAGGGCAGTGCCCTGGAAGACATCGAACACCTCAAGGACATGGC
CTCCGAAGCGCCGGTGATTCGCCTGGTCAACCTGATCCTGCAGCGCGCGGTGGAACATCGTGCCTCGGATATTCATATCG
AACCCTTCGAAAACCAGCTCAAGGTGCGCTACCGCATCGACGGCGTGCTGCACGAAGCCGAAGCCCCGCCGTCCAGTTCT
TCGGCGGCGGTGATTTCGCGGGTGAAGATCATGGCGCGCCTGGACATCGCCGAGCGGCGCCTGCCCCAGGACGGGCGCAT
CATGCTGCGCATCCAGGGCAAGGAGCTGGACTTGCGGGTGTCCACGGTGCCCACCAGTTTTGGCGAATCGGTGGTCATGC
GTTTGCTCGACCGCCAGACCGTGCAATTCGATTTCCAGAGCCTGGGCTTTGATGGCCAGCGCCTGGAAACCTTCCTCGAA
GTGCTCGAGCGGCCCCACGGCATCCTGCTGGTCACCGGGCCCACCGGCTCGGGCAAGACCACCACCCTGTACACCGCGCT
GTCGCGGCTCAATACCGCCGAGCGCAAGATCATCACCGTCGAAGACCCGGTGGAATACCAGCTTGAAGGCATCAACCAGA
TCCAGGTCAAGCCGGCCATCGGCCTGGACTTCGCCGGTGCGCTGCGCTCCATCGTGCGCCAGGACCCGGACGTGATCATG
ATCGGTGAAATCCGCGACCTGGAAACCTGCCGCATCGCCATCCAGTCGTCCCTCACCGGCCACCTGGTGCTATCGACCCT
GCACACCAACAGCGCGGCCGCGAGTATCACGCGCTTGCTCGACATGGGCGTCGAAAGCTACCTGATTGCCTCGACCGTCA
ACGGCATCCTCGCCCAGCGCCTGGTGCGACGCCTGGACCCGGCCACCCGCGAAGCCTTCGAAGCCCCTGCGGAATTGATC
GCCGAGCACGGCCTGGATCGGTTTACCGAGCAGCGCCCGATCCTGCTCTATCGCCCCCGCGCCGATGCGCCGGGCGGTGG
CTACCACGGCCGCAGTGCGATCACCGAACTGCTGGTGATGAACGACGAACTGCGCAGCCTGCTGATGCGCCAGGCCGACG
CCGCCACCCTCGAACAGGCCGCCCGCCGTGGCGGCCTGCGCACCTTGCATGAAGAAGGCCTGCGCCAGGCCGTGGCGGGT
GTCACCTCCCTTGAAGAAGTGCTGCGCGTCACCCGTGGAGAGGGCGCATGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-161)

Type II/IV secretion system protein (T2SSE)

(198-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

39.33

98.438

0.387
  pilB Acinetobacter baumannii D1279779

38.194

100

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.858

93.056

0.38
  pilB Vibrio cholerae strain A1552

43.878

85.069

0.373
  pilB Legionella pneumophila strain ERS1305867

39.777

93.403

0.372
  pilB Vibrio campbellii strain DS40M4

44.375

83.333

0.37
  pilB Acinetobacter baylyi ADP1

37.083

98.785

0.366
  pilF Neisseria gonorrhoeae MS11

38.561

94.097

0.363
  pilB Vibrio parahaemolyticus RIMD 2210633

43.333

83.333

0.361