Detailed information NTG0153124

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: V471_RS01995
• Genome accession: NZ_CP015283
• Coordinates: 394157..395257 (-)
• Length: 366 a.a.
• NCBI ID: WP_138261574.1
• Uniprot ID: -
• Organism: Streptococcus salivarius strain ATCC 25975
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 389157..400257

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V471_RS01955 (V471_01960)	-	389766..389963 (-)	198	WP_002885716.1	helix-turn-helix transcriptional regulator	-
V471_RS01960 (V471_01965)	-	390214..391407 (-)	1194	WP_003095894.1	acetate kinase	-
V471_RS01965 (V471_01970)	comGH	391464..392420 (-)	957	WP_037601477.1	class I SAM-dependent methyltransferase	Machinery gene
V471_RS01970 (V471_01975)	comGG	392465..392782 (-)	318	WP_037601475.1	competence type IV pilus minor pilin ComGG	Machinery gene
V471_RS01975 (V471_01980)	comGF	392760..393197 (-)	438	WP_037601472.1	competence type IV pilus minor pilin ComGF	Machinery gene
V471_RS01980 (V471_01985)	comGE	393184..393414 (-)	231	WP_084871057.1	competence type IV pilus minor pilin ComGE	Machinery gene
V471_RS01985 (V471_01990)	comGD	393446..393874 (-)	429	WP_014632541.1	competence type IV pilus minor pilin ComGD	Machinery gene
V471_RS01990 (V471_01995)	comGC	393834..394148 (-)	315	WP_037611125.1	competence type IV pilus major pilin ComGC	Machinery gene
V471_RS01995 (V471_02000)	comGB	394157..395257 (-)	1101	WP_138261574.1	competence type IV pilus assembly protein ComGB	Machinery gene
V471_RS02000 (V471_02005)	comGA/cglA/cilD	395139..396080 (-)	942	WP_002887018.1	competence type IV pilus ATPase ComGA	Machinery gene
V471_RS02005 (V471_02010)	-	396114..397592 (-)	1479	WP_084871058.1	IS1182 family transposase	-
V471_RS02010 (V471_02015)	-	397722..398084 (-)	363	WP_049529260.1	DUF1033 family protein	-

Sequence

Protein

• Length: 366 a.a.
• Molecular weight: 41751.02 Da
• Isoelectric Point: 10.1478

>NTDB_id=153124 V471_RS01995 WP_138261574.1 394157..395257(-) (comGB) [Streptococcus salivarius strain ATCC 25975]
MPVKISKVIRQPVGTSSWKAWLNKDISLKGISKGKKLKINQQLKVIQLFKQLLKAGFTLTEIVAFLERSHLLKESSLSLM
KASLMRGDRLDQMFASVGFSDNIVTQIALADKHGNLLGSLTKIETYMLRMTKVRKKLMEVATYPILLLGFLILIMLGLKN
YLLPQLLEGDGKENWAVQLVQIFPQLFFVILCGLLVLSLILYLWVKRQSALVFYRRMAKIPFIGQTVRLYMTAYYAREWG
NLLGQGIDLLDLVALMKEQKSKLFRELGADLEEALMLGQSFPDRIASHPFFTKELSLIIAYGEANARLGYELEVYAEEVW
QTFFNRLNKATTFVQPLIFVIVAVVIVMIYAAMLLPMYQNMEGMMS

Nucleotide

• Length: 1101 bp

>NTDB_id=153124 V471_RS01995 WP_138261574.1 394157..395257(-) (comGB) [Streptococcus salivarius strain ATCC 25975]
TTGCCAGTGAAAATTTCCAAAGTCATTCGTCAACCAGTTGGAACCAGCAGTTGGAAGGCTTGGTTAAACAAGGATATCTC
ACTGAAGGGGATATCCAAGGGGAAAAAATTAAAGATTAATCAGCAACTCAAGGTTATCCAGCTTTTCAAACAACTTTTAA
AGGCAGGGTTTACCTTGACTGAAATCGTAGCCTTTTTGGAGCGAAGTCACTTGTTGAAAGAATCGTCCTTGTCTCTTATG
AAAGCGAGTTTAATGCGAGGTGACAGGTTGGACCAGATGTTTGCGTCAGTGGGCTTTTCGGACAATATTGTTACTCAGAT
TGCCCTTGCTGATAAGCACGGTAATCTTCTAGGGAGTTTAACTAAGATTGAAACCTACATGCTTCGTATGACCAAGGTTC
GTAAGAAACTCATGGAGGTAGCGACCTACCCTATTCTACTTCTGGGTTTCCTGATTCTGATTATGTTGGGGCTCAAAAAT
TATCTTCTGCCTCAACTGTTAGAGGGGGATGGTAAGGAGAATTGGGCTGTACAGTTGGTTCAAATTTTTCCCCAGCTCTT
TTTTGTGATTTTGTGCGGACTGCTTGTATTAAGCTTAATTCTCTATCTATGGGTGAAACGCCAGTCAGCCCTTGTCTTTT
ATCGACGAATGGCAAAAATCCCTTTTATAGGCCAAACTGTCAGGCTATATATGACCGCCTATTATGCTAGGGAATGGGGA
AATCTCTTAGGGCAAGGTATTGACTTGCTAGACTTAGTTGCCCTAATGAAAGAGCAAAAATCAAAGCTCTTTCGTGAGCT
GGGGGCTGATTTAGAAGAAGCCTTGATGCTAGGACAGAGTTTTCCTGACCGTATTGCTAGTCACCCTTTCTTCACTAAGG
AACTATCCTTAATTATTGCTTATGGAGAGGCTAATGCTAGGTTGGGCTATGAGTTAGAAGTCTATGCTGAAGAGGTTTGG
CAGACTTTCTTCAACCGTCTTAATAAGGCAACAACCTTTGTGCAACCCCTCATTTTTGTTATTGTTGCTGTCGTGATTGT
AATGATCTATGCAGCCATGCTATTACCAATGTATCAAAATATGGAAGGAATGATGTCATGA

Domains

Predicted by InterProScan.

• Type II secretion system (T2SS), protein F (T2SSF) (51-164)

• Type II secretion system (T2SS), protein F (T2SSF) (236-356)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Streptococcus mutans UA140	55.394	93.716	0.519
comGB	Streptococcus mutans UA159	55.394	93.716	0.519
comGB	Streptococcus gordonii str. Challis substr. CH1	51.312	93.716	0.481
comGB/cglB	Streptococcus mitis NCTC 12261	50	92.35	0.462
comGB/cglB	Streptococcus mitis SK321	49.408	92.35	0.456
comGB/cglB	Streptococcus pneumoniae Rx1	49.112	92.35	0.454
comGB/cglB	Streptococcus pneumoniae D39	49.112	92.35	0.454
comGB/cglB	Streptococcus pneumoniae R6	49.112	92.35	0.454
comGB/cglB	Streptococcus pneumoniae TIGR4	49.112	92.35	0.454
comGB	Lactococcus lactis subsp. cremoris KW2	44.985	89.891	0.404