Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A5955_RS06350 Genome accession   NZ_CP015241
Coordinates   1274171..1275664 (-) Length   497 a.a.
NCBI ID   WP_000249366.1    Uniprot ID   -
Organism   Escherichia coli strain 2013C-4465     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1269171..1280664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A5955_RS06320 (A5955_06315) gspK 1269965..1270942 (-) 978 WP_000633162.1 type II secretion system minor pseudopilin GspK -
  A5955_RS06325 (A5955_06320) gspJ 1270939..1271544 (-) 606 WP_001255032.1 type II secretion system minor pseudopilin GspJ -
  A5955_RS06330 (A5955_06325) gspI 1271541..1271912 (-) 372 WP_000820134.1 type II secretion system minor pseudopilin GspI -
  A5955_RS06335 (A5955_06330) gspH 1271909..1272472 (-) 564 WP_001115148.1 type II secretion system minor pseudopilin GspH -
  A5955_RS06340 (A5955_06335) gspG 1272476..1272931 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  A5955_RS06345 (A5955_06340) gspF 1272948..1274171 (-) 1224 WP_001173397.1 type II secretion system inner membrane protein GspF -
  A5955_RS06350 (A5955_06345) pilB 1274171..1275664 (-) 1494 WP_000249366.1 type II secretion system ATPase GspE Machinery gene
  A5955_RS06355 (A5955_06350) gspD 1275664..1277724 (-) 2061 WP_000498829.1 type II secretion system secretin GspD -
  A5955_RS06360 (A5955_06355) gspC 1277754..1278713 (-) 960 WP_000135080.1 type II secretion system protein GspC -
  A5955_RS06365 (A5955_06360) gspS2 1278731..1279141 (-) 411 WP_001345954.1 type II secretion system pilot lipoprotein GspS-beta -
  A5955_RS06370 (A5955_06365) pilD 1279207..1280016 (-) 810 WP_000895883.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55106.25 Da        Isoelectric Point: 6.1631

>NTDB_id=152839 A5955_RS06350 WP_000249366.1 1274171..1275664(-) (pilB) [Escherichia coli strain 2013C-4465]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDGAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=152839 A5955_RS06350 WP_000249366.1 1274171..1275664(-) (pilB) [Escherichia coli strain 2013C-4465]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGGGGCCGAATTTGAACAGCGACTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCTATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATTCACATCGAGACATTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCGCGGCTGGATATTGCCGAAAAGCGCGTACC
GCAGGATGGGCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATTGCCGTCCAGGCATCGCTTACAGGGCACCT
GGTACTTTCCACTCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACAGGTGTGATGGCGCAGCGGCTGGTTCGGACGCTGTGTCCCGACTGCCGTCAGGCCGCGCCTGCG
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCGCAAGGCTGCCCCGCCTG
TAATCACAAAGGTTTCCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTAGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTGCTGCGGGTCACGATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.13

99.396

0.439
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilB Acinetobacter baylyi ADP1

46.154

78.471

0.362
  pilF Thermus thermophilus HB27

45

80.483

0.362