Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GJ11_RS21100 Genome accession   NZ_CP015229
Coordinates   3845535..3847028 (-) Length   497 a.a.
NCBI ID   WP_065227658.1    Uniprot ID   -
Organism   Escherichia coli strain 06-00048     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3840535..3852028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJ11_RS21070 (GJ11_19545) gspK 3841329..3842306 (-) 978 WP_065227653.1 type II secretion system minor pseudopilin GspK -
  GJ11_RS21075 (GJ11_19550) gspJ 3842303..3842908 (-) 606 WP_065227654.1 type II secretion system minor pseudopilin GspJ -
  GJ11_RS21080 (GJ11_19555) gspI 3842905..3843276 (-) 372 WP_065227655.1 type II secretion system minor pseudopilin GspI -
  GJ11_RS21085 (GJ11_19560) gspH 3843273..3843836 (-) 564 WP_024165236.1 type II secretion system minor pseudopilin GspH -
  GJ11_RS21090 (GJ11_19565) gspG 3843840..3844295 (-) 456 WP_065227656.1 type II secretion system major pseudopilin GspG -
  GJ11_RS21095 (GJ11_19570) gspF 3844312..3845535 (-) 1224 WP_065227657.1 type II secretion system inner membrane protein GspF -
  GJ11_RS21100 (GJ11_19575) pilB/pilB1 3845535..3847028 (-) 1494 WP_065227658.1 type II secretion system ATPase GspE Machinery gene
  GJ11_RS21105 (GJ11_19580) gspD 3847028..3849088 (-) 2061 WP_000498829.1 type II secretion system secretin GspD -
  GJ11_RS21110 (GJ11_19585) gspC 3849118..3850077 (-) 960 WP_065227659.1 type II secretion system protein GspC -
  GJ11_RS21115 (GJ11_19590) gspS2 3850093..3850503 (-) 411 WP_065227660.1 type II secretion system pilot lipoprotein GspS-beta -
  GJ11_RS21120 (GJ11_19595) pilD 3850569..3851378 (-) 810 WP_065227661.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55237.39 Da        Isoelectric Point: 6.0956

>NTDB_id=152609 GJ11_RS21100 WP_065227658.1 3845535..3847028(-) (pilB/pilB1) [Escherichia coli strain 06-00048]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AHLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLIRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=152609 GJ11_RS21100 WP_065227658.1 3845535..3847028(-) (pilB/pilB1) [Escherichia coli strain 06-00048]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGACGCGCCAATCATCAAACTGATCAACGCTATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATTGAGACATTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCGCGGCTGGATATTGCCGAAAAGCGCGTACC
GCAGGATGGACGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGCG
AGCGCGTGGTACTGCGATTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTGAGTCATGAACTGACC
GCGCATTTGCGCCAGCTGTTACACAAACCGCACGGTATCTTTCTGGTGACGGGGCCGACAGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCACTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTCAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCGTCGCTCACTGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATTACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGGCTGATTCGCACGCTGTGCCCCGACTGCCGCCAGCCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTACCGACGCGCGTACTGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGGCGTACTGCCATCCATGAGTTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTGCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

44.75

80.483

0.36

Multiple sequence alignment