Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GJ11_RS21120 Genome accession   NZ_CP015229
Coordinates   3850569..3851378 (-) Length   269 a.a.
NCBI ID   WP_065227661.1    Uniprot ID   -
Organism   Escherichia coli strain 06-00048     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3845569..3856378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJ11_RS21105 (GJ11_19580) gspD 3847028..3849088 (-) 2061 WP_000498829.1 type II secretion system secretin GspD -
  GJ11_RS21110 (GJ11_19585) gspC 3849118..3850077 (-) 960 WP_065227659.1 type II secretion system protein GspC -
  GJ11_RS21115 (GJ11_19590) gspS2 3850093..3850503 (-) 411 WP_065227660.1 type II secretion system pilot lipoprotein GspS-beta -
  GJ11_RS21120 (GJ11_19595) pilD 3850569..3851378 (-) 810 WP_065227661.1 prepilin peptidase PppA Machinery gene
  GJ11_RS21125 (GJ11_19600) sslE 3851572..3856131 (-) 4560 WP_065227662.1 lipoprotein metalloprotease SslE -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29494.05 Da        Isoelectric Point: 8.3793

>NTDB_id=152610 GJ11_RS21120 WP_065227661.1 3850569..3851378(-) (pilD) [Escherichia coli strain 06-00048]
MHFDVFQQYPAVMPILATVGGLIIGSFLNVVIWRYPIMLRQQMAEFQGETPSAQSKISLALPRSHCPHCQQTIRVRDNIP
LFSWLMLKGRCRDCQTKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSWVGPLSLPNVALIASCCGLIYAV
ITKKGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=152610 GJ11_RS21120 WP_065227661.1 3850569..3851378(-) (pilD) [Escherichia coli strain 06-00048]
ATGCATTTTGATGTTTTTCAGCAATATCCAGCGGTGATGCCCATCCTGGCAACCGTTGGAGGATTAATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTTCGCCAACAAATGGCGGAGTTTCAGGGGGAAACGCCGAGTGCGC
AGTCAAAAATAAGCCTGGCGCTGCCACGTTCGCACTGTCCGCATTGCCAGCAGACCATCCGCGTTCGTGACAATATTCCG
CTATTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGACGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTCTGGCCGGAAAGTGGATGGGGGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGTGTCATCGACCTCGATAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACG
GGACTAATTGCGGCATGGGCGCAGCAGAGCCCGCTCACGCTACAAGATGCAGTCACCGGCGTCCTGGTGGGGTTTATCAC
TTTTTACTCTCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTATTGTTCGCTG
CGTTAGGTAGTTGGGTGGGGCCGTTGTCACTGCCCAATGTTGCTTTAATCGCCTCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAAAGGATCAACGACGCTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.318

99.257

0.45
  pilD Vibrio campbellii strain DS40M4

40.659

100

0.413
  pilD Neisseria gonorrhoeae MS11

39.922

95.911

0.383
  pilD Acinetobacter nosocomialis M2

38.113

98.513

0.375
  pilD Acinetobacter baumannii D1279779

37.736

98.513

0.372