Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AC609_RS15460 Genome accession   NZ_CP012048
Coordinates   3377977..3379014 (-) Length   345 a.a.
NCBI ID   WP_127446505.1    Uniprot ID   A0A3Q9Q5H0
Organism   Xanthomonas phaseoli pv. phaseoli strain ISO18C2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3372977..3384014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC609_RS15440 (AC609_14740) - 3373861..3374433 (+) 573 WP_017157669.1 DNA-3-methyladenine glycosylase I -
  AC609_RS15445 (AC609_14745) - 3374441..3375376 (+) 936 WP_017157670.1 DUF72 domain-containing protein -
  AC609_RS15450 (AC609_14750) - 3375544..3376218 (-) 675 WP_039573830.1 YitT family protein -
  AC609_RS15455 (AC609_14755) pilU 3376724..3377854 (-) 1131 WP_008571827.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AC609_RS15460 (AC609_14760) pilT 3377977..3379014 (-) 1038 WP_127446505.1 type IV pilus twitching motility protein PilT Machinery gene
  AC609_RS15465 (AC609_14765) - 3379433..3380125 (+) 693 WP_039573827.1 YggS family pyridoxal phosphate-dependent enzyme -
  AC609_RS15470 (AC609_14770) proC 3380169..3381023 (+) 855 WP_039573824.1 pyrroline-5-carboxylate reductase -
  AC609_RS15475 (AC609_14775) - 3381580..3382005 (+) 426 WP_017159922.1 HU family DNA-binding protein -
  AC609_RS15480 (AC609_14780) - 3382111..3382752 (+) 642 WP_017157674.1 M23 family metallopeptidase -
  AC609_RS15485 (AC609_14785) - 3382810..3383151 (+) 342 WP_003489901.1 DUF3861 family protein -
  AC609_RS15490 (AC609_14790) - 3383385..3383741 (+) 357 WP_080948968.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38662.34 Da        Isoelectric Point: 6.7747

>NTDB_id=150858 AC609_RS15460 WP_127446505.1 3377977..3379014(-) (pilT) [Xanthomonas phaseoli pv. phaseoli strain ISO18C2]
MDIAELLAFSVKNKASDLRLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=150858 AC609_RS15460 WP_127446505.1 3377977..3379014(-) (pilT) [Xanthomonas phaseoli pv. phaseoli strain ISO18C2]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCGCCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCGCTGGACCATAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAGGAATTCCTCGAGGTCGACTTTTCGTTCGAGATTCCGTCGCTGGCGCGT
TTTCGCGTCAACGCGTTCAACCAGAACCGCGGTGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAAGTGTTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAATGAGTACGGGCATATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGTTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTCACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCAGTGATTTCGCAGGC
GCTGTTGAAGAAGGTCGGCGGCGGCCGTACGGCGGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCCCGCGAGTACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q9Q5H0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment