Detailed information NTG0150858

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: PA8281_RS12280
• Genome accession: NZ_CP015002
• Coordinates: 2600657..2601328 (+)
• Length: 223 a.a.
• NCBI ID: WP_003090494.1
• Uniprot ID: A0A0H2ZC88
• Organism: Pseudomonas aeruginosa strain PA8281
• Function: repress competence development (predicted from homology) Competence regulation

Genomic Context

Location: 2595657..2606328

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA8281_RS12245 (PA8281_04447)	ppyR	2596130..2596387 (+)	258	WP_003090502.1	psl/pyoverdine operon transcriptional regulator PpyR	-
PA8281_RS12250 (PA8281_04446)	-	2596374..2597567 (+)	1194	WP_019726389.1	NnrS family protein	-
PA8281_RS12255 (PA8281_06548)	-	2597568..2597834 (-)	267	WP_003115326.1	DUF5329 domain-containing protein	-
PA8281_RS12260 (PA8281_04445)	-	2597818..2598711 (-)	894	WP_003116712.1	hypothetical protein	-
PA8281_RS12265 (PA8281_04444)	-	2598738..2599820 (-)	1083	WP_003113385.1	hypothetical protein	-
PA8281_RS12270 (PA8281_04443)	-	2600035..2600343 (+)	309	WP_003090497.1	PepSY domain-containing protein	-
PA8281_RS12275 (PA8281_04442)	-	2600343..2600657 (+)	315	WP_003097704.1	PepSY domain-containing protein	-
PA8281_RS12280 (PA8281_04441)	ciaR	2600657..2601328 (+)	672	WP_003090494.1	two-component system response regulator CarR	Regulator
PA8281_RS12285 (PA8281_04440)	carS	2601325..2602662 (+)	1338	WP_003090493.1	two-component system sensor histidine kinase CarS	-
PA8281_RS12290 (PA8281_04439)	-	2602753..2603061 (+)	309	WP_003116713.1	hypothetical protein	-
PA8281_RS34330	-	2603066..2603602 (-)	537	Protein_2453	methyl-accepting chemotaxis protein	-
PA8281_RS34335	-	2603972..2605210 (-)	1239	Protein_2454	chemotaxis protein	-

Sequence

Protein

• Length: 223 a.a.
• Molecular weight: 24729.20 Da
• Isoelectric Point: 5.8445

>NTDB_id=150858 PA8281_RS12280 WP_003090494.1 2600657..2601328(+) (ciaR) [Pseudomonas aeruginosa strain PA8281]
MRLLLVEDHVPLADELMASLTRQGYAVDWLADGRDAAVQGASEPYDLIILDLGLPGRPGLEILQEWRGLGLATPVLILTA
RGSWAERIDGLKAGADDYLTKPFHPEELALRIQALLRRAHGLANQSQLEAAGLRLDEQRQCVCLNGADVDLTAAEFRLLR
YFMLHPGQVLSKGHLAEHLYDGETERDSNVIEVHVNHLRRKLGREVIETRRGQGYRYAGVAAG

Nucleotide

• Length: 672 bp

>NTDB_id=150858 PA8281_RS12280 WP_003090494.1 2600657..2601328(+) (ciaR) [Pseudomonas aeruginosa strain PA8281]
ATGCGGTTGCTGCTGGTTGAGGATCACGTTCCCCTGGCCGACGAACTGATGGCCAGCCTGACCCGCCAGGGCTACGCGGT
GGACTGGCTGGCCGACGGCCGCGACGCGGCGGTGCAGGGCGCCAGCGAGCCCTACGACCTGATCATCCTCGACCTCGGCC
TGCCCGGGCGCCCCGGCCTGGAGATACTCCAGGAGTGGCGTGGGCTGGGACTCGCGACTCCGGTGCTGATCCTCACCGCG
CGCGGTTCCTGGGCCGAACGCATCGATGGGCTCAAGGCCGGTGCCGACGACTATCTGACCAAGCCGTTCCATCCGGAAGA
GTTGGCCTTGCGCATCCAGGCCCTGTTGCGCCGCGCCCATGGGCTGGCCAACCAGAGCCAGCTGGAGGCCGCCGGGCTGC
GCCTGGACGAGCAGCGTCAGTGCGTGTGCCTGAACGGCGCCGACGTCGACCTGACCGCCGCCGAATTCCGCCTGCTGCGC
TATTTCATGCTGCACCCCGGCCAGGTCCTGTCCAAGGGGCACCTGGCCGAGCATCTGTACGACGGCGAGACCGAACGCGA
TTCCAACGTCATCGAGGTCCACGTCAACCACCTGCGGCGCAAGCTCGGCCGCGAGGTGATCGAGACCCGCCGTGGCCAGG
GCTACCGCTACGCCGGGGTGGCCGCCGGATGA

Domains

Predicted by InterProScan.

• Response regulator receiver domain (Response_reg) (4-112)

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (147-216)

3D structure

• AlphaFold DB: A0A0H2ZC88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus mutans UA159	37.727	98.655	0.372
ciaR	Streptococcus pneumoniae Rx1	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae D39	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae R6	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae TIGR4	37.273	98.655	0.368
micA	Streptococcus pneumoniae Cp1015	35.683	100	0.363
vicR	Streptococcus mutans UA159	35.526	100	0.363