Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   ACJ67_RS00720 Genome accession   NZ_CP012020
Coordinates   156248..156850 (-) Length   200 a.a.
NCBI ID   WP_053092870.1    Uniprot ID   -
Organism   Methylophilus sp. TWE2     
Function   DNA uptake (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 157001..187218 156248..156850 flank 151


Gene organization within MGE regions


Location: 156248..187218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ67_RS00720 (ACJ67_00720) comM 156248..156850 (-) 603 WP_053092870.1 ATP-binding protein Machinery gene
  ACJ67_RS00725 (ACJ67_00725) - 157001..157369 (-) 369 WP_019880923.1 hypothetical protein -
  ACJ67_RS00730 (ACJ67_00730) - 157366..157740 (-) 375 WP_019880925.1 HTH domain-containing protein -
  ACJ67_RS00735 (ACJ67_00735) - 157788..158216 (-) 429 WP_019880926.1 hypothetical protein -
  ACJ67_RS00740 (ACJ67_00740) - 158341..158697 (+) 357 WP_019880927.1 helix-turn-helix domain-containing protein -
  ACJ67_RS00745 (ACJ67_00745) - 158871..160460 (-) 1590 WP_019880928.1 hypothetical protein -
  ACJ67_RS00750 (ACJ67_00750) - 160501..160686 (-) 186 WP_019880929.1 hypothetical protein -
  ACJ67_RS00755 (ACJ67_00755) - 161197..161646 (-) 450 WP_019880930.1 DUF6527 family protein -
  ACJ67_RS00760 (ACJ67_00760) - 161636..162805 (-) 1170 WP_019880931.1 ThiF family adenylyltransferase -
  ACJ67_RS00765 (ACJ67_00765) - 162780..163061 (-) 282 WP_019880932.1 multiubiquitin domain-containing protein -
  ACJ67_RS00770 (ACJ67_00770) - 163190..163777 (+) 588 WP_019880933.1 sigma-70 family RNA polymerase sigma factor -
  ACJ67_RS00775 (ACJ67_00775) - 163774..164178 (+) 405 WP_019880936.1 hypothetical protein -
  ACJ67_RS00780 (ACJ67_00780) - 164666..165094 (+) 429 WP_019880937.1 ProQ/FinO family protein -
  ACJ67_RS00785 (ACJ67_00785) - 165201..165923 (+) 723 WP_019880938.1 ZIP family metal transporter -
  ACJ67_RS00790 (ACJ67_00790) - 166039..166485 (+) 447 WP_231587210.1 MgtC/SapB family protein -
  ACJ67_RS00795 (ACJ67_00795) - 166838..167893 (-) 1056 WP_049637478.1 DMT family transporter -
  ACJ67_RS00800 (ACJ67_00800) flgB 167933..168373 (-) 441 WP_019880941.1 flagellar basal body rod protein FlgB -
  ACJ67_RS00805 (ACJ67_00805) - 168409..170655 (-) 2247 WP_231587211.1 cation-translocating P-type ATPase -
  ACJ67_RS00810 (ACJ67_00810) - 170753..173842 (-) 3090 WP_049637480.1 efflux RND transporter permease subunit -
  ACJ67_RS00815 (ACJ67_00815) - 173852..175033 (-) 1182 WP_019880945.1 efflux RND transporter periplasmic adaptor subunit -
  ACJ67_RS00820 (ACJ67_00820) - 175043..176320 (-) 1278 WP_231587212.1 TolC family protein -
  ACJ67_RS00825 (ACJ67_00825) - 176387..176752 (-) 366 WP_049637481.1 hypothetical protein -
  ACJ67_RS00830 (ACJ67_00830) - 177038..178735 (+) 1698 WP_019880948.1 phosphoethanolamine transferase -
  ACJ67_RS00835 (ACJ67_00835) - 178747..180168 (-) 1422 WP_019880950.1 site-specific integrase -
  ACJ67_RS00840 (ACJ67_00840) - 180181..180708 (-) 528 WP_019880951.1 hypothetical protein -
  ACJ67_RS00845 (ACJ67_00845) - 180701..182650 (-) 1950 WP_231587214.1 hypothetical protein -
  ACJ67_RS00850 (ACJ67_00850) - 182838..184373 (-) 1536 WP_049637482.1 SulP family inorganic anion transporter -
  ACJ67_RS00855 (ACJ67_00855) - 184659..184925 (-) 267 WP_049637483.1 hypothetical protein -
  ACJ67_RS00860 (ACJ67_00860) - 184915..187218 (-) 2304 WP_049637484.1 hypothetical protein -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 22856.25 Da        Isoelectric Point: 9.3760

>NTDB_id=150413 ACJ67_RS00720 WP_053092870.1 156248..156850(-) (comM) [Methylophilus sp. TWE2]
MVKSKIPEFDRKVLEVLREPLESGHITISRAARQATFPAQFQLIAAMNPCPCGYYGHYNQRCRCTPEQILRYKNKLSGPL
LDRIDMNIEVPALKPEEISHPHPGESSMSMRSRVELARQRQLQRQQKTNAALGPMEISAFCQLSEQANHLLQTALQKFSL
SARSYHRILKVGRTIADLADSPHISDAHIAEALQYRRYDY

Nucleotide


Download         Length: 603 bp        

>NTDB_id=150413 ACJ67_RS00720 WP_053092870.1 156248..156850(-) (comM) [Methylophilus sp. TWE2]
ATGGTAAAAAGCAAAATACCTGAATTTGATCGCAAAGTGTTGGAAGTGCTGCGTGAACCCTTGGAAAGTGGACATATCAC
CATCTCTCGGGCTGCCAGGCAAGCGACTTTTCCAGCCCAATTCCAACTGATTGCAGCCATGAACCCTTGCCCTTGTGGCT
ATTACGGCCATTACAACCAGCGCTGCCGCTGCACTCCGGAACAGATTCTTCGTTACAAAAACAAACTTTCGGGCCCCCTA
TTGGACCGCATTGACATGAATATAGAGGTCCCTGCGCTTAAGCCGGAAGAAATCAGCCATCCTCATCCTGGTGAAAGCAG
CATGAGCATGCGCTCCAGAGTGGAGCTTGCCAGACAGCGACAACTGCAACGCCAGCAAAAAACGAATGCGGCATTGGGCC
CAATGGAAATTTCTGCCTTTTGCCAATTGAGTGAACAGGCCAACCACCTGTTACAGACGGCCTTACAAAAGTTCTCGCTT
TCGGCCCGCAGCTATCACCGCATCCTGAAAGTGGGCAGGACTATTGCCGACCTGGCTGACTCGCCACATATTAGCGATGC
ACACATTGCGGAAGCGCTACAATATCGGCGTTATGACTATTGA

Domains

Predicted by InterproScan.

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(6-97)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(104-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Vibrio campbellii strain DS40M4

52.041

98

0.51
  comM Glaesserella parasuis strain SC1401

51.515

99

0.51
  comM Vibrio cholerae strain A1552

51.02

98

0.5
  comM Haemophilus influenzae Rd KW20

51.031

97

0.495
  comM Legionella pneumophila strain ERS1305867

49.744

97.5

0.485
  comM Legionella pneumophila str. Paris

49.744

97.5

0.485
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

48.744

99.5

0.485

Multiple sequence alignment