Detailed information NTG0149355

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACP86_RS13480
• Genome accession: NZ_CP011929
• Coordinates: 2917173..2918234 (+)
• Length: 353 a.a.
• NCBI ID: WP_053114371.1
• Uniprot ID: A0A0K1UBD9
• Organism: Marinobacter sp. CP1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2912173..2923234

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACP86_RS13465 (ACP86_13460)	rpmE	2912645..2912866 (+)	222	WP_008170804.1	50S ribosomal protein L31	-
ACP86_RS13470 (ACP86_13465)	-	2913118..2914383 (+)	1266	WP_053114367.1	malic enzyme-like NAD(P)-binding protein	-
ACP86_RS13475 (ACP86_13470)	-	2914449..2916977 (-)	2529	WP_053114369.1	penicillin-binding protein 1A	-
ACP86_RS13480 (ACP86_13475)	pilM	2917173..2918234 (+)	1062	WP_053114371.1	pilus assembly protein PilM	Machinery gene
ACP86_RS13485 (ACP86_13480)	-	2918234..2918797 (+)	564	WP_053114373.1	PilN domain-containing protein	-
ACP86_RS13490 (ACP86_13485)	-	2918797..2919408 (+)	612	WP_008170795.1	type 4a pilus biogenesis protein PilO	-
ACP86_RS13495 (ACP86_13490)	-	2919411..2919959 (+)	549	WP_008170793.1	pilus assembly protein PilP	-
ACP86_RS13500 (ACP86_13495)	pilQ	2920025..2922094 (+)	2070	WP_176443082.1	type IV pilus secretin PilQ	Machinery gene
ACP86_RS13505 (ACP86_13500)	aroK	2922231..2922797 (+)	567	WP_008170790.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 37825.91 Da
• Isoelectric Point: 4.1452

>NTDB_id=149355 ACP86_RS13480 WP_053114371.1 2917173..2918234(+) (pilM) [Marinobacter sp. CP1]
MFGLGKKSSAVLGVDISSSSVKLLELSKQGDRYKVESYAVEPLPANAVVEKNITDVEAVGEVMKRVASKSRSGVKQVAVA
VSGSAVITKLIQMDGGLNEFEMEDQIALEADQYIPYPLDEVAIDFEVQGPSENNPDQVDVLLAACRKENVDMREDALEIA
SLTTKVVDVEAYALERAYALIEPQLDSQGEELVVAIVDIGATMTTLSVLAGGKTVYTREQIFGGKQLTEEIQRRYGLSLE
EAGLAKKQGGLPDDYESEVLTPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIQGLTEMVEEKTGTPTMVANPF
ADMAVGSKVNASALSNDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=149355 ACP86_RS13480 WP_053114371.1 2917173..2918234(+) (pilM) [Marinobacter sp. CP1]
GTGTTTGGATTAGGAAAGAAATCCAGTGCAGTGCTGGGCGTGGATATAAGCTCCAGCTCGGTCAAACTTCTGGAGCTATC
GAAGCAGGGCGACCGTTATAAGGTCGAAAGCTACGCGGTGGAGCCTTTGCCGGCCAATGCCGTGGTGGAAAAGAACATTA
CGGACGTAGAAGCTGTGGGCGAGGTAATGAAGCGCGTAGCTTCCAAGTCCCGTTCTGGTGTCAAACAGGTGGCTGTTGCG
GTCTCTGGTTCTGCGGTTATTACCAAGCTTATCCAGATGGATGGCGGCCTGAACGAATTCGAGATGGAAGATCAGATTGC
CCTTGAGGCCGATCAATATATTCCATACCCGCTGGATGAAGTGGCGATCGACTTCGAGGTTCAGGGACCTTCCGAGAACA
ACCCCGACCAGGTGGATGTGCTTCTGGCTGCTTGCCGGAAGGAAAACGTCGACATGCGCGAGGATGCACTCGAGATAGCT
TCACTGACTACCAAGGTGGTTGACGTTGAAGCCTATGCCCTTGAGCGTGCCTATGCACTGATCGAGCCTCAGCTGGACTC
TCAGGGAGAGGAGCTGGTGGTAGCGATCGTGGACATTGGCGCGACCATGACCACGCTGAGTGTTCTGGCCGGTGGCAAGA
CCGTTTACACGCGAGAGCAGATTTTCGGTGGCAAACAGCTGACCGAGGAAATCCAGCGCCGATACGGTTTGTCGCTCGAG
GAAGCCGGTCTTGCGAAAAAGCAGGGCGGACTGCCTGACGATTACGAGTCAGAAGTGCTTACGCCGTTCCGCGAGGCTGT
CGTGCAGCAGGTTGCCCGCGCGCTTCAGTTCTTCTTTGGTGCCAGTCAATACAATGCCGTCGATTACGTGGTTCTGGCCG
GAGGCACGGCCTCGATTCAGGGGCTCACTGAAATGGTCGAAGAAAAAACGGGAACGCCGACCATGGTCGCGAATCCTTTT
GCAGATATGGCGGTAGGTTCAAAGGTGAACGCGTCGGCACTGAGTAATGATGCGCCTTCCCTGATGATTGCCTGCGGCCT
GGCAATGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

3D structure

• AlphaFold DB: A0A0K1UBD9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	54.023	98.584	0.533
comM	Acinetobacter nosocomialis M2	54.023	98.584	0.533
comM	Acinetobacter baylyi ADP1	51.724	98.584	0.51
pilM	Legionella pneumophila strain ERS1305867	46.571	99.15	0.462