Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABA45_RS02790 Genome accession   NZ_CP011494
Coordinates   633713..634834 (+) Length   373 a.a.
NCBI ID   WP_048384255.1    Uniprot ID   A0A0H4I1H2
Organism   Marinobacter psychrophilus strain 20041     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 628713..639834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABA45_RS02770 (ABA45_02770) - 630251..630835 (-) 585 WP_048384251.1 YggT family protein -
  ABA45_RS02775 (ABA45_02775) proC 630861..631691 (-) 831 WP_048384252.1 pyrroline-5-carboxylate reductase -
  ABA45_RS02780 (ABA45_02780) - 631764..632468 (-) 705 WP_048384253.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABA45_RS02785 (ABA45_02785) pilT 632605..633639 (+) 1035 WP_048384254.1 type IV pilus twitching motility protein PilT Machinery gene
  ABA45_RS02790 (ABA45_02790) pilU 633713..634834 (+) 1122 WP_048384255.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABA45_RS02795 (ABA45_02795) - 634953..636383 (+) 1431 WP_084708264.1 OmpP1/FadL family transporter -
  ABA45_RS02800 (ABA45_02800) - 636417..636782 (-) 366 WP_048384256.1 hypothetical protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41409.60 Da        Isoelectric Point: 6.5030

>NTDB_id=146329 ABA45_RS02790 WP_048384255.1 633713..634834(+) (pilU) [Marinobacter psychrophilus strain 20041]
MEFEKLLRLMVEKGGSDLFITAGVAPSMKVNGKVWPVTKTALTPDMTRELVYGAMSDKQRAEFDETHECNFAISARGIGR
FRVSAFFQRNQCGMVLRRIEVKIPEIDDLSLPNIVKELAMTKRGLIMFVGATGTGKSTSLAAMIGFRNHNSRGHIISIED
PIEFIHQHQGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRTRQTMEYSVQFAETGHLCLATLHANNANQALDRI
IQFFPPEQHGQIWMDLSLNLRAIVAQQLVPTPDGKGRKAVIEVLINTPLAADLIRKGDVHKLKDLMAKSNEIGMRTFDQA
LYELYSEGSVTYEDALAHADSANDLRLMIKLGTDAQGADKLSSSVDKLTIQQD

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=146329 ABA45_RS02790 WP_048384255.1 633713..634834(+) (pilU) [Marinobacter psychrophilus strain 20041]
ATGGAATTTGAAAAGCTACTGCGGCTGATGGTTGAGAAAGGCGGCTCGGACTTGTTCATTACCGCCGGCGTTGCGCCCAG
TATGAAAGTGAACGGTAAGGTGTGGCCTGTCACAAAGACTGCCCTCACACCCGATATGACTCGCGAGTTAGTTTACGGCG
CCATGAGCGACAAGCAACGCGCTGAGTTTGATGAAACTCACGAGTGCAACTTTGCCATCAGCGCCCGTGGCATTGGTCGC
TTTCGGGTTAGTGCGTTTTTCCAGCGTAATCAGTGCGGTATGGTGCTGCGTCGTATTGAAGTAAAGATACCGGAAATTGA
CGATCTTTCACTGCCCAACATCGTCAAAGAGTTGGCTATGACCAAGCGCGGGCTGATCATGTTTGTCGGCGCCACCGGTA
CAGGAAAATCTACCTCGTTGGCTGCGATGATTGGCTTCCGCAACCACAACAGCCGCGGCCATATCATTTCTATTGAAGAT
CCGATTGAGTTTATCCACCAACATCAGGGTTGCATTGTGACCCAGCGCGAAGTGGGCATAGACACCGACAGCTTTGAGGT
GGCGCTGAAAAACACCCTGCGCCAGGCGCCAGATGTGATTTTGATTGGTGAGGTGCGAACCCGCCAGACCATGGAATATT
CGGTACAGTTCGCCGAAACCGGTCACTTGTGCCTGGCGACTCTGCACGCCAACAACGCCAACCAGGCTCTGGACCGGATT
ATCCAGTTTTTTCCGCCCGAACAGCACGGCCAAATCTGGATGGACCTGTCGCTGAATCTGCGTGCCATTGTGGCGCAGCA
ACTGGTGCCCACGCCAGACGGCAAGGGCCGGAAAGCGGTTATTGAAGTGCTGATCAATACCCCGCTGGCGGCCGACCTGA
TTCGCAAGGGCGATGTGCACAAGCTTAAAGATCTGATGGCTAAATCCAACGAAATTGGCATGCGCACTTTTGATCAGGCG
CTGTATGAGCTGTATTCCGAAGGTTCAGTGACTTATGAAGATGCTCTGGCACATGCCGATTCAGCCAACGATTTGCGTTT
GATGATAAAGCTTGGAACCGACGCCCAAGGGGCGGACAAGTTGTCGTCTTCGGTGGACAAGCTGACCATTCAGCAGGATT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H4I1H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

78.437

99.464

0.78
  pilU Acinetobacter baylyi ADP1

65.73

95.442

0.627
  pilU Vibrio cholerae strain A1552

54.865

99.196

0.544
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.845

95.174

0.389

Multiple sequence alignment