Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   SpyM6D471_RS06465 Genome accession   NZ_CP011415
Coordinates   1279932..1281830 (-) Length   632 a.a.
NCBI ID   WP_002994648.1    Uniprot ID   A0A5S4TL28
Organism   Streptococcus pyogenes strain D471     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 1274932..1286830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SpyM6D471_RS06440 (SpyM6D471_06435) - 1275668..1276045 (-) 378 WP_002991882.1 S1 RNA-binding domain-containing protein -
  SpyM6D471_RS06445 (SpyM6D471_06440) - 1276045..1277445 (-) 1401 WP_011888672.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  SpyM6D471_RS06450 (SpyM6D471_06445) - 1277482..1278123 (-) 642 WP_002983671.1 response regulator transcription factor -
  SpyM6D471_RS06455 (SpyM6D471_06450) - 1278116..1279120 (-) 1005 WP_011184815.1 sensor histidine kinase -
  SpyM6D471_RS06460 (SpyM6D471_06455) liaF 1279117..1279809 (-) 693 WP_011054833.1 cell wall-active antibiotics response protein LiaF -
  SpyM6D471_RS06465 (SpyM6D471_06460) pknB 1279932..1281830 (-) 1899 WP_002994648.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  SpyM6D471_RS06470 (SpyM6D471_06465) - 1281827..1282567 (-) 741 WP_002983660.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  SpyM6D471_RS06475 (SpyM6D471_06470) rsmB 1282605..1283927 (-) 1323 WP_011184816.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  SpyM6D471_RS06480 (SpyM6D471_06475) fmt 1283917..1284852 (-) 936 WP_011184817.1 methionyl-tRNA formyltransferase -

Sequence

Protein


Download         Length: 632 a.a.        Molecular weight: 68819.06 Da        Isoelectric Point: 7.2899

>NTDB_id=145818 SpyM6D471_RS06465 WP_002994648.1 1279932..1281830(-) (pknB) [Streptococcus pyogenes strain D471]
MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVLRTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIG
EEDGQQFLVMEYVDGADLKRYIQNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAVTIALQHFQKPLPSIIEENHNVP
QALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYNRSRERKIVFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESR
LDQTNQTDALQPPTKKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDVGLKVGKIR
QIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDYQEAMNSLIETYGVPKSKIKIERIVTNEYPE
NTVISQSPSAGDKFNPNGKSKITLSVAVSDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSS
KAIVSGQSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSPSETTAQTP

Nucleotide


Download         Length: 1899 bp        

>NTDB_id=145818 SpyM6D471_RS06465 WP_002994648.1 1279932..1281830(-) (pknB) [Streptococcus pyogenes strain D471]
ATGATTCAGATTGGCAAATTATTTGCTGGTCGTTATCGCATTCTGAAATCTATTGGCCGCGGTGGTATGGCGGATGTTTA
TTTAGCAAATGATTTGATCTTGGATAATGAAGACGTTGCAATCAAGGTCTTGCGTACCAATTATCAAACAGATCAGGTAG
CAGTTGCGCGTTTCCAACGGGAAGCGCGGGCCATGGCTGAATTGAACCATCCCAATATTGTTGCCATCCGGGATATAGGT
GAAGAAGACGGACAGCAATTTTTAGTAATGGAATATGTGGATGGTGCTGACCTAAAGAGATACATTCAAAATCATGCTCC
ATTATCTAATAATGAAGTGGTTAGAATTATGGAAGAAGTCCTTTCTGCTATGACTTTAGCCCACCAAAAAGGAATTGTAC
ACAGAGATTTAAAACCTCAAAATATCCTACTAACTAAGGAGGGGGTTGTCAAAGTAACTGATTTCGGCATCGCAGTAGCC
TTTGCAGAAACAAGCTTGACACAAACTAATTCGATGTTAGGCAGTGTTCATTACTTGTCTCCAGAACAGGCTCGCGGCTC
CAAAGCGACGATTCAAAGTGATATTTATGCGATGGGGATTATGCTCTTTGAGATGTTGACAGGCCATATCCCTTATGATG
GGGATAGTGCTGTTACGATTGCCTTGCAACATTTTCAAAAGCCTCTTCCATCTATTATCGAGGAGAACCACAATGTGCCA
CAAGCTTTGGAGAATGTTGTTATTCGAGCAACAGCCAAGAAATTAAGTGATCGTTACGGGTCAACCTTTGAAATGAGTCG
TGACTTAATGACGGCGCTTAGTTATAATCGTAGTCGGGAGCGTAAGATTGTCTTTGAGGATGTTGAAAGTACCAAACCCC
TCCCCAAAGTGGCCTCAGGCCCCACCGCTTCTGTAAAATTGTCTCCCCCTACCCCAACAGTGTTAACACAGGAAAGTCGA
TTAGATCAAACTAATCAAACAGATGCTTTACAGCCCCCCACCAAAAAGAAAAAAAGTGGTCGTTTTTTAGGTACTTTATT
CAAAATTCTTTTTTCTTTCTTTATTGTAGGTGTAGCACTCTTTACTTATCTTATACTAACTAAACCAACTTCTGTGAAAG
TTCCTAATGTAGCAGGCACTAGTCTTAAAGTTGCCAAACAAGAACTGTATGATGTTGGGCTAAAAGTGGGTAAAATCAGG
CAAATTGAGAGTGATACGGTTGCTGAGGGAAATGTAGTTAGAACAGATCCTAAAGCAGGAACAGCTAAGAGGCAAGGCTC
AAGCATTACGCTTTATGTGTCAATTGGAAACAAAGGTTTTGACATGGAAAACTACAAAGGACTAGATTATCAAGAAGCTA
TGAATAGTTTGATAGAAACTTATGGTGTTCCAAAATCAAAAATCAAAATTGAACGCATTGTAACTAATGAATATCCTGAA
AATACAGTCATCAGTCAATCGCCAAGTGCGGGTGATAAATTTAATCCAAACGGAAAGTCTAAAATTACGCTCAGTGTTGC
TGTTAGTGATACGATCACTATGCCTATGGTAACAGAATATAGTTATGCAGATGCAGTCAATACCTTAACAGCTTTAGGTA
TAGATGCATCTAGAATAAAAGCTTATGTGCCAAGCTCTAGCTCAGCAACGGGCTTTGTGCCAATTCATTCTCCTAGTTCT
AAAGCTATTGTCAGTGGTCAATCTCCTTACTATGGAACGTCTTTGAGTCTGTCTGATAAAGGAGAAATTAGTCTTTACCT
TTATCCAGAAGAAACACACTCTTCTAGTAGCTCATCGAGTTCAACGTCAAGTTCAAACAGTTCTTCAACAAATGATAGTA
CTGCACCAGGTAGCAACACTGAATTAAGCCCATCAGAAACTACTGCTCAAACACCTTAA

Domains

Predicted by InterproScan.

Protein kinase domain (Pkinase)

(12-242)

PASTA domain (PASTA)

(372-434)

PASTA domain (PASTA)

(441-506)

PASTA domain (PASTA)

(512-588)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TL28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

65.424

93.354

0.611
  stkP/pknB Streptococcus salivarius strain HSISS4

61.513

94.146

0.579
  stkP Streptococcus pneumoniae TIGR4

57.171

81.646

0.467
  stkP Streptococcus pneumoniae D39

56.977

81.646

0.465
  stkP Streptococcus pneumoniae R6

56.977

81.646

0.465
  stkP Streptococcus pneumoniae Rx1

56.783

81.646

0.464

Multiple sequence alignment