Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAX10_RS08980 Genome accession   NZ_CP011381
Coordinates   1937333..1938988 (+) Length   551 a.a.
NCBI ID   WP_046700412.1    Uniprot ID   -
Organism   Moraxella bovoculi strain 58086     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1932333..1943988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAX10_RS08950 (AAX10_08810) nusA 1933462..1934943 (-) 1482 WP_046700410.1 transcription termination factor NusA -
  AAX10_RS08955 (AAX10_08815) rimP 1934988..1935485 (-) 498 WP_036367169.1 ribosome maturation factor RimP -
  AAX10_RS08970 (AAX10_08830) secG 1936020..1936334 (-) 315 WP_036367167.1 preprotein translocase subunit SecG -
  AAX10_RS08975 (AAX10_08835) tpiA 1936378..1937115 (-) 738 WP_046700411.1 triose-phosphate isomerase -
  AAX10_RS08980 (AAX10_08840) pilB 1937333..1938988 (+) 1656 WP_046700412.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAX10_RS08985 (AAX10_08845) - 1938981..1940228 (+) 1248 WP_080947148.1 type II secretion system F family protein -
  AAX10_RS08990 (AAX10_08850) - 1940225..1941127 (+) 903 WP_046700413.1 prepilin peptidase -
  AAX10_RS08995 (AAX10_08855) coaE 1941176..1941814 (+) 639 WP_046700414.1 dephospho-CoA kinase -
  AAX10_RS09000 (AAX10_08860) - 1941811..1942347 (-) 537 WP_046700705.1 DUF924 family protein -
  AAX10_RS09005 (AAX10_08865) - 1942382..1942822 (-) 441 WP_080947149.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 551 a.a.        Molecular weight: 60259.76 Da        Isoelectric Point: 6.4631

>NTDB_id=145319 AAX10_RS08980 WP_046700412.1 1937333..1938988(+) (pilB) [Moraxella bovoculi strain 58086]
MNHATTGLARIILDKALINHDVLGAAIDDASAAQISLARHLVRHGVLSSDVIAKTLSESLGMDLVDLDKFNIKELPLHLL
DDALIARHEVLPLGQRDGQIFIATSDPTRAQVMSELGFATNAHITPVLVDENKLVRIMQSLHLGAVLELGEDDHELPDND
GEAMLDAPTVKFVHKTLSDAIIMGASDIHFEPFDKHYRVRFRIDGIMQSIANPPKASGNKISTRLKVMARLDIAERRKPQ
DGRIKFPLHEGKSVDFRVSTTPTLFGEKIVLRLLDSAQALIGIDTLGMNDAQKQLFLDALNRPQGMIVITGPTGSGKTIS
LYTALGILNRPEINIQTVEDPVEIHLDGINQVNINPKIGLDFADVLRAFLRQDPDVIMVGEIRDVQTAEIAIKAAQTGHL
VLSTLHTNSAIDTIIRLKNMGVATFNITSSLSLIIAQRLVRRLCGDCKQPLDIPAQSLIELGFSPDQIGTAKLYKPVGCH
QCKDGYKGRIGIYEMLPITPKLATLIMSDAPTQALSEFAKEHGMMTLRQSAMHQVISGITSLQEMERLTGA

Nucleotide


Download         Length: 1656 bp        

>NTDB_id=145319 AAX10_RS08980 WP_046700412.1 1937333..1938988(+) (pilB) [Moraxella bovoculi strain 58086]
ATGAACCACGCCACCACAGGACTTGCTCGAATTATACTAGATAAAGCACTCATCAATCATGACGTGCTTGGGGCTGCCAT
TGATGACGCTAGTGCCGCGCAGATTAGCCTTGCTCGCCATCTAGTGCGACATGGTGTACTGTCCTCCGATGTGATCGCCA
AGACTTTATCTGAGAGTCTGGGCATGGACTTGGTGGATTTGGATAAATTTAACATCAAAGAGCTGCCCTTGCATCTGCTT
GATGATGCACTCATTGCACGCCACGAGGTGTTACCCCTTGGTCAGCGCGATGGGCAAATTTTTATCGCCACCAGTGACCC
GACACGCGCGCAAGTAATGAGCGAGCTTGGCTTTGCAACCAATGCGCACATCACGCCCGTTCTGGTCGATGAGAACAAAC
TCGTACGCATCATGCAAAGCCTGCATCTAGGCGCGGTGCTAGAGCTTGGCGAGGACGATCATGAACTGCCTGATAATGAC
GGTGAGGCGATGCTTGATGCGCCGACCGTTAAATTCGTCCACAAGACATTAAGTGATGCCATCATCATGGGTGCATCAGA
CATTCATTTTGAGCCGTTCGATAAGCATTATCGGGTAAGATTTCGCATTGATGGCATCATGCAGAGCATCGCAAACCCTC
CCAAGGCTTCAGGCAATAAAATCAGTACTCGCCTAAAAGTCATGGCACGTCTCGACATTGCCGAACGACGAAAGCCGCAA
GATGGGCGCATCAAATTTCCCCTGCATGAAGGCAAATCGGTTGATTTTCGTGTCAGTACCACGCCCACACTCTTTGGCGA
GAAGATCGTCTTAAGGCTACTAGATTCTGCCCAAGCCCTAATCGGCATTGATACGCTCGGCATGAATGATGCCCAAAAAC
AGCTGTTCCTTGATGCACTAAACCGTCCTCAAGGCATGATCGTCATCACAGGCCCGACAGGCTCTGGTAAGACAATCTCG
CTATACACCGCGCTTGGCATCCTAAATCGACCCGAGATTAACATCCAAACCGTCGAAGACCCTGTGGAAATCCACCTAGA
TGGCATCAATCAGGTCAATATCAACCCAAAGATTGGGCTTGACTTTGCTGATGTGCTAAGAGCTTTCTTGCGTCAAGATC
CCGACGTCATCATGGTGGGCGAGATTCGCGACGTACAGACCGCAGAGATTGCCATTAAGGCCGCTCAGACAGGCCACTTG
GTGCTGTCCACATTACACACCAATTCTGCCATCGACACCATCATTCGCCTAAAGAACATGGGCGTGGCAACCTTTAATAT
CACAAGCTCCCTAAGTCTCATCATCGCCCAAAGGCTCGTCCGCCGTCTATGCGGTGACTGTAAGCAGCCCTTAGACATCC
CCGCCCAAAGCCTGATTGAGCTTGGCTTTAGTCCAGATCAAATCGGCACAGCCAAGCTATATAAGCCCGTCGGTTGCCAT
CAATGTAAAGACGGCTACAAAGGACGCATAGGCATCTATGAGATGCTGCCCATCACCCCTAAGCTCGCCACGCTCATCAT
GAGCGACGCGCCGACTCAGGCACTTAGCGAATTTGCCAAAGAACACGGCATGATGACCCTTAGACAATCTGCCATGCATC
AGGTCATCAGCGGCATCACAAGCCTACAAGAGATGGAGCGTCTCACAGGTGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(175-442)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

47.872

100

0.49
  pilB Acinetobacter baylyi ADP1

47.772

100

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

47.331

100

0.483
  pilB Legionella pneumophila strain ERS1305867

49.442

97.641

0.483
  pilB Vibrio campbellii strain DS40M4

46.441

100

0.474
  pilB Vibrio cholerae strain A1552

46.346

100

0.472
  pilF Neisseria gonorrhoeae MS11

44.304

100

0.445
  pilF Thermus thermophilus HB27

36.957

100

0.37

Multiple sequence alignment