Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAX08_RS09320 Genome accession   NZ_CP011380
Coordinates   1986628..1988283 (+) Length   551 a.a.
NCBI ID   WP_046698716.1    Uniprot ID   -
Organism   Moraxella bovoculi strain 57922     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1981628..1993283
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAX08_RS09290 (AAX08_09175) nusA 1982753..1984234 (-) 1482 WP_036367173.1 transcription termination factor NusA -
  AAX08_RS09295 (AAX08_09180) rimP 1984283..1984780 (-) 498 WP_036367169.1 ribosome maturation factor RimP -
  AAX08_RS09310 (AAX08_09195) secG 1985313..1985627 (-) 315 WP_036367167.1 preprotein translocase subunit SecG -
  AAX08_RS09315 (AAX08_09200) tpiA 1985671..1986408 (-) 738 WP_046699038.1 triose-phosphate isomerase -
  AAX08_RS09320 (AAX08_09205) pilB 1986628..1988283 (+) 1656 WP_046698716.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAX08_RS09325 (AAX08_09210) - 1988276..1989523 (+) 1248 WP_118873533.1 type II secretion system F family protein -
  AAX08_RS09330 (AAX08_09215) - 1989520..1990422 (+) 903 WP_046698717.1 prepilin peptidase -
  AAX08_RS09335 (AAX08_09220) coaE 1990471..1991109 (+) 639 WP_046698718.1 dephospho-CoA kinase -
  AAX08_RS09340 (AAX08_09225) - 1991106..1991642 (-) 537 WP_046699039.1 DUF924 family protein -
  AAX08_RS09345 (AAX08_09230) - 1991677..1992225 (-) 549 WP_162888307.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 551 a.a.        Molecular weight: 60272.78 Da        Isoelectric Point: 6.3774

>NTDB_id=145306 AAX08_RS09320 WP_046698716.1 1986628..1988283(+) (pilB) [Moraxella bovoculi strain 57922]
MNHATTGLARIILDKALINHDVLGAAIDDASAAQISLARHLVRHGVLSSDVIAKTLSESLGMDLVDLDKFNIKELPLHLL
DDALIARHEVLPLGQRDGQIFIATSDPTRAQVMSELGFATNAHITPVLVDENKLVRIMQSLHLGAVLELGEDDHELPDSD
GEAMLDAPTVKFVHKTLSDAIIMGASDIHFEPFDKHYRVRFRIDGIMQTIANPPKASGNKISTRLKVMARLDIAERRKPQ
DGRIKFPLHEGKSVDFRVSTTPTLFGEKIVLRLLDSAQALIGIDTLGMNDAQKQLFLDALNRPQGMIVITGPTGSGKTIS
LYTALGILNRPEINIQTVEDPVEIHLDGINQVNINPKIGLDFADVLRAFLRQDPDVIMVGEIRDVQTAEIAIKAAQTGHL
VLSTLHTNSAIDTIIRLKNMGVATFNITSSLSLIIAQRLVRRLCGDCKQLLDIPAQSLIELGFSPDQIGAAKLYKPVGCH
QCKDGYKGRIGIYEILPITPKLATLIMSDAPTQALSEFAKEHDMMTLRQSAMHQVISGITSLQEMERLTGA

Nucleotide


Download         Length: 1656 bp        

>NTDB_id=145306 AAX08_RS09320 WP_046698716.1 1986628..1988283(+) (pilB) [Moraxella bovoculi strain 57922]
ATGAACCACGCCACCACAGGACTTGCTCGAATTATACTAGATAAAGCACTCATCAATCATGACGTGCTTGGGGCTGCCAT
TGATGACGCTAGTGCCGCGCAGATTAGCCTTGCTCGCCATCTAGTGCGACATGGCGTACTGTCCTCCGATGTGATCGCCA
AGACTTTATCTGAGAGTCTGGGCATGGACTTGGTGGATTTGGATAAATTTAACATCAAAGAGCTGCCCTTGCATCTGCTT
GATGATGCACTCATTGCACGCCACGAGGTGTTACCCCTTGGTCAGCGCGATGGGCAAATTTTTATCGCCACCAGTGACCC
GACACGCGCGCAAGTAATGAGCGAGCTTGGCTTTGCAACCAATGCGCACATCACGCCCGTTCTGGTCGATGAGAACAAAC
TCGTACGCATCATGCAAAGCCTGCATCTAGGCGCGGTGCTAGAGCTTGGCGAGGACGATCATGAACTGCCTGATAGTGAC
GGTGAGGCGATGCTTGATGCGCCGACCGTTAAATTCGTCCACAAGACATTAAGTGATGCCATCATCATGGGTGCATCAGA
CATTCATTTTGAGCCGTTCGATAAGCATTATCGGGTAAGATTTCGCATTGATGGCATCATGCAGACCATCGCAAACCCTC
CCAAGGCTTCAGGCAATAAAATCAGTACCCGCCTAAAAGTCATGGCACGTCTCGACATTGCCGAACGACGAAAGCCGCAA
GATGGGCGCATCAAATTTCCCCTGCATGAAGGCAAATCGGTTGATTTTCGTGTCAGTACCACGCCCACACTCTTTGGCGA
GAAGATCGTCTTAAGGCTACTAGATTCTGCCCAAGCCCTAATCGGCATTGATACGCTCGGCATGAATGATGCCCAAAAAC
AGCTGTTCCTTGATGCACTAAACCGTCCTCAAGGCATGATCGTCATCACAGGCCCGACAGGCTCTGGTAAGACAATCTCG
CTATACACCGCGCTTGGTATCCTAAATCGACCCGAAATTAACATCCAAACCGTCGAAGACCCTGTGGAAATTCACCTAGA
TGGCATCAATCAGGTCAATATCAACCCAAAGATTGGGCTTGACTTTGCTGATGTGCTAAGAGCTTTCTTGCGTCAAGATC
CCGACGTCATCATGGTGGGCGAGATTCGCGACGTACAGACCGCAGAGATTGCCATTAAGGCCGCTCAGACAGGCCACTTG
GTGCTGTCCACATTACACACCAATTCTGCCATCGACACCATCATTCGCCTAAAGAACATGGGCGTGGCGACCTTTAATAT
CACAAGCTCCCTAAGTCTCATCATCGCCCAAAGGCTCGTCCGCCGTCTATGCGGTGACTGTAAGCAGCTCTTAGACATTC
CCGCCCAAAGCCTGATTGAGCTTGGCTTTAGCCCAGATCAAATCGGCGCAGCCAAGCTATATAAGCCCGTCGGTTGCCAT
CAATGCAAAGACGGCTACAAAGGACGCATAGGCATCTATGAGATACTGCCCATCACCCCCAAGCTCGCCACGCTCATCAT
GAGCGACGCGCCGACTCAGGCACTTAGCGAATTTGCCAAAGAACACGACATGATGACCCTTAGACAATCTGCCATGCATC
AGGTCATCAGCGGCATCACAAGCCTACAAGAGATGGAGCGTCTCACAGGTGCCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-139)

Type II/IV secretion system protein (T2SSE)

(175-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

47.518

100

0.486
  pilB Acinetobacter baylyi ADP1

47.415

100

0.483
  pilB Legionella pneumophila strain ERS1305867

49.257

97.641

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

46.975

100

0.479
  pilB Vibrio cholerae strain A1552

46.168

100

0.47
  pilB Vibrio campbellii strain DS40M4

46.085

100

0.47
  pilF Neisseria gonorrhoeae MS11

44.123

100

0.443
  pilF Thermus thermophilus HB27

36.775

100

0.368

Multiple sequence alignment