Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAX09_RS09140 Genome accession   NZ_CP011374
Coordinates   1962447..1964102 (+) Length   551 a.a.
NCBI ID   WP_046701482.1    Uniprot ID   -
Organism   Moraxella bovoculi strain 58069     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1957447..1969102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAX09_RS09110 (AAX09_09110) nusA 1958576..1960057 (-) 1482 WP_046700410.1 transcription termination factor NusA -
  AAX09_RS09115 (AAX09_09115) rimP 1960102..1960599 (-) 498 WP_036367169.1 ribosome maturation factor RimP -
  AAX09_RS09130 (AAX09_09130) secG 1961134..1961448 (-) 315 WP_036367167.1 preprotein translocase subunit SecG -
  AAX09_RS09135 (AAX09_09135) tpiA 1961492..1962229 (-) 738 WP_046701744.1 triose-phosphate isomerase -
  AAX09_RS09140 (AAX09_09140) pilB 1962447..1964102 (+) 1656 WP_046701482.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAX09_RS09145 (AAX09_09145) - 1964095..1965342 (+) 1248 WP_080947375.1 type II secretion system F family protein -
  AAX09_RS09150 (AAX09_09150) - 1965339..1966241 (+) 903 WP_046701483.1 A24 family peptidase -
  AAX09_RS09155 (AAX09_09155) coaE 1966290..1966928 (+) 639 WP_046701484.1 dephospho-CoA kinase -
  AAX09_RS09160 (AAX09_09160) - 1966925..1967461 (-) 537 WP_046700705.1 DUF924 family protein -
  AAX09_RS09165 (AAX09_09165) - 1967496..1967936 (-) 441 WP_080947149.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 551 a.a.        Molecular weight: 60236.79 Da        Isoelectric Point: 6.6708

>NTDB_id=145234 AAX09_RS09140 WP_046701482.1 1962447..1964102(+) (pilB) [Moraxella bovoculi strain 58069]
MNHATTGLARIILDKALINHDVLGAAIDDASAAQISLARHLVRHGVLSSDVIAKTLSESLGIDLVDLDKFNIKELPLHLL
DDALIARHEVLPLGQRDGQIFIATSDPTRAQVMSELGFATNAHITPILVDENKLVRIMQSLHLGAVLELGEDDHELPDSD
GEAMLDAPTVKFVHKTLSDAIIMGASDIHFEPFDKHYRVRFRIDGIMQSIANPPKASGNKISTRLKVMARLDIAERRKPQ
DGRIKFPLHEGKSVDFRVSTTPTLFGEKIVLRLLDSAQALIGIDTLGMNDAQKQLFLDALNRPQGMIVITGPTGSGKTIS
LYTALGILNRPEINIQTVEDPVEIHLDGINQVNINPKIGLDFADVLRAFLRQDPDVIMVGEIRDVQTAEIAIKAAQTGHL
VLSTLHTNSAIDTIIRLKNMGVATFNITSSLSLIIAQRLVRRLCGDCKQPLDIPAQSLIELGFSPDQIGTAKLYKPVGCH
QCKDGYKGRIGIYEMLPITPKLATLIMSDAPTQALSEFAKKHGMMTLRHSAMHQVISGITSLQEMERLTGA

Nucleotide


Download         Length: 1656 bp        

>NTDB_id=145234 AAX09_RS09140 WP_046701482.1 1962447..1964102(+) (pilB) [Moraxella bovoculi strain 58069]
ATGAACCACGCCACCACAGGACTTGCTCGAATTATACTAGATAAAGCACTCATCAATCATGACGTGCTTGGGGCTGCCAT
TGATGACGCTAGTGCCGCGCAGATTAGCCTTGCTCGCCATCTAGTGCGACATGGCGTACTGTCCTCCGATGTGATCGCCA
AGACTTTATCTGAAAGTCTGGGCATAGACTTGGTGGATTTGGATAAATTTAACATCAAAGAGCTGCCCTTGCATCTGCTT
GATGATGCACTCATTGCACGCCACGAGGTGTTACCCCTTGGTCAGCGCGATGGGCAAATTTTTATCGCCACCAGTGACCC
GACACGCGCGCAAGTAATGAGCGAGCTTGGCTTTGCAACCAATGCGCACATCACGCCCATTCTGGTCGATGAGAATAAAC
TCGTACGCATCATGCAAAGCCTGCATCTAGGCGCGGTGCTAGAGCTTGGCGAGGACGATCATGAACTGCCTGATAGTGAC
GGTGAGGCGATGCTTGATGCGCCGACCGTTAAATTCGTCCACAAGACATTAAGTGATGCCATCATCATGGGTGCATCAGA
CATTCATTTTGAGCCGTTCGATAAGCATTATCGGGTAAGATTTCGCATTGATGGCATCATGCAGAGCATCGCAAACCCTC
CCAAGGCTTCAGGCAATAAAATCAGTACTCGCCTAAAAGTCATGGCACGTCTCGACATTGCCGAACGACGAAAGCCGCAA
GATGGGCGCATCAAATTTCCCCTGCATGAAGGTAAATCGGTTGATTTTCGTGTCAGTACCACGCCCACACTCTTTGGCGA
GAAGATCGTCTTAAGGCTACTAGATTCTGCCCAAGCCCTAATCGGCATTGATACGCTCGGCATGAATGATGCCCAAAAAC
AGCTGTTCCTTGATGCACTAAACCGTCCTCAAGGCATGATCGTCATCACAGGCCCGACAGGCTCTGGTAAGACAATCTCG
CTATACACCGCGCTTGGTATCCTAAATCGACCCGAAATTAACATCCAAACCGTCGAAGACCCTGTGGAAATTCACCTAGA
TGGCATCAATCAGGTCAATATCAACCCAAAGATTGGGCTTGACTTTGCTGATGTGCTAAGAGCTTTCTTGCGTCAAGATC
CCGACGTCATCATGGTGGGCGAGATTCGCGACGTACAGACCGCAGAGATTGCCATTAAGGCCGCTCAGACAGGCCACTTG
GTGCTGTCCACACTACACACCAATTCTGCCATCGACACCATCATTCGCCTAAAGAACATGGGCGTGGCAACCTTTAATAT
CACAAGCTCCCTAAGTCTCATCATCGCCCAAAGGCTTGTCCGCCGTCTATGCGGTGACTGTAAGCAGCCCTTAGACATCC
CCGCCCAAAGCCTGATTGAGCTTGGCTTTAGCCCAGATCAAATCGGCACAGCCAAGCTATATAAGCCCGTCGGTTGCCAT
CAATGCAAAGACGGCTACAAAGGACGCATAGGCATCTATGAGATGCTGCCCATCACCCCTAAGCTCGCCACACTCATCAT
GAGCGACGCACCGACTCAGGCACTTAGCGAATTTGCCAAAAAACACGGCATGATGACCCTTAGACATTCTGCCATGCATC
AGGTCATCAGCGGCATCACAAGCCTACAAGAGATGGAGCGTCTCACAGGTGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(175-442)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

47.872

100

0.49
  pilB Acinetobacter baylyi ADP1

47.95

100

0.488
  pilB Legionella pneumophila strain ERS1305867

49.442

97.641

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

47.153

100

0.481
  pilB Vibrio cholerae strain A1552

46.346

100

0.472
  pilB Vibrio campbellii strain DS40M4

46.263

100

0.472
  pilF Neisseria gonorrhoeae MS11

44.304

100

0.445
  pilF Thermus thermophilus HB27

36.957

100

0.37

Multiple sequence alignment