Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AXE82_RS04015 Genome accession   NZ_CP014234
Coordinates   923762..925267 (-) Length   501 a.a.
NCBI ID   WP_172460486.1    Uniprot ID   A0A378Q921
Organism   Moraxella osloensis strain CCUG 350     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 918762..930267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXE82_RS03990 (AXE82_03990) - 920629..921390 (+) 762 WP_062331730.1 class I SAM-dependent methyltransferase -
  AXE82_RS03995 (AXE82_03995) miaB 921464..922930 (+) 1467 WP_062331733.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  AXE82_RS04000 (AXE82_04000) - 922935..923249 (+) 315 WP_062331735.1 hypothetical protein -
  AXE82_RS04005 (AXE82_04005) - 923260..923460 (-) 201 WP_062331738.1 hypothetical protein -
  AXE82_RS04010 (AXE82_04010) - 923450..923716 (-) 267 WP_062331741.1 BrnT family toxin -
  AXE82_RS04015 (AXE82_04015) pilB 923762..925267 (-) 1506 WP_172460486.1 type II secretion system ATPase GspE Machinery gene
  AXE82_RS04020 (AXE82_04020) - 925441..926292 (+) 852 WP_227713382.1 thiazole synthase -
  AXE82_RS04025 (AXE82_04025) - 926320..927678 (+) 1359 WP_062331744.1 WS/DGAT/MGAT family O-acyltransferase -
  AXE82_RS04030 (AXE82_04030) - 927751..928479 (-) 729 WP_147285573.1 amino acid ABC transporter ATP-binding protein -
  AXE82_RS04035 (AXE82_04035) - 928596..929267 (-) 672 WP_050324714.1 ABC transporter permease subunit -
  AXE82_RS04040 (AXE82_04040) - 929264..930010 (-) 747 WP_062331749.1 amino acid ABC transporter permease -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55460.66 Da        Isoelectric Point: 5.5178

>NTDB_id=144809 AXE82_RS04015 WP_172460486.1 923762..925267(-) (pilB) [Moraxella osloensis strain CCUG 350]
MLNPIALPYSFAKRHQLLLQYSQDLTAPPTLLMTADTPMEAINEANRFASQAGVPLPFTFAMAQSDDFEKQLAAVYAGNT
GESQHIAAGLEDHPDLMSLADSVPETEDLMDQEDDAPIIRLINALLSEAIRLNASDIHIETFEKRLSVRFRVDGILKEIV
SPKRELSPLLVSRIKVMAKMDIAEKRVPQDGRISLRLAGREVDVRVSTLPSNFGERVVMRLLDKQAGRLNMTYLGLSDND
YSELKRLIHRPHGIILVTGPTGSGKTTTLYAALTDLNDNTRNILTAEDPIEFQLDGIGQTQVNNKVDMTFARSLRAMLRQ
DPDVVMVGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLQDMGVEPFLLSSSLIGVVAQRLVRTLCPHCHTWTL
ADAYQTQIFTEIGEVGEIKLPKPVGCEKCNQSGFRGRTAIYEVVPVDDKLRQLIHSQTAEFELEAYARSKTPSIRADGLK
KVLAGKTTLEEVLRVTKEKEM

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=144809 AXE82_RS04015 WP_172460486.1 923762..925267(-) (pilB) [Moraxella osloensis strain CCUG 350]
ATGCTAAATCCGATTGCCTTGCCCTATAGTTTTGCCAAACGTCATCAACTGCTGCTGCAATACTCGCAAGATTTGACTGC
CCCGCCGACGTTATTGATGACGGCGGATACCCCTATGGAAGCCATCAACGAAGCCAACCGTTTTGCCAGTCAAGCAGGGG
TGCCACTGCCATTTACTTTTGCCATGGCACAAAGTGACGATTTTGAAAAGCAGTTAGCCGCGGTCTATGCAGGCAACACT
GGGGAATCTCAGCATATTGCCGCTGGGCTTGAAGACCATCCCGATTTGATGAGTCTGGCTGACAGTGTCCCTGAGACGGA
AGACTTGATGGACCAAGAAGATGACGCGCCCATCATTCGGCTGATTAATGCGCTACTGTCTGAAGCCATCCGCCTAAACG
CCTCAGATATTCACATCGAAACCTTTGAAAAACGGCTATCGGTGCGCTTTCGGGTGGATGGGATACTCAAAGAAATCGTC
AGTCCAAAACGGGAACTGTCACCGCTACTGGTATCGCGTATCAAGGTGATGGCAAAAATGGATATCGCTGAAAAACGGGT
GCCGCAAGATGGACGCATCTCATTACGTCTGGCTGGGCGAGAGGTGGATGTGCGGGTATCGACCTTGCCATCAAATTTTG
GTGAGCGTGTGGTGATGCGGCTCTTGGATAAACAAGCGGGTCGGTTAAATATGACCTATTTGGGGTTGTCAGACAATGAT
TATAGCGAGCTTAAACGCTTAATCCATCGCCCGCATGGCATTATTCTTGTCACAGGTCCGACAGGTTCGGGCAAAACCAC
GACGCTGTACGCGGCGTTGACGGATTTGAACGATAACACCCGTAATATTTTGACCGCTGAAGACCCGATTGAATTTCAGC
TAGATGGTATTGGGCAAACCCAAGTCAATAATAAAGTGGATATGACCTTTGCCCGTAGTTTGCGAGCCATGCTACGCCAA
GACCCTGACGTGGTGATGGTGGGGGAGATTCGCGATTTAGAGACCGCAGAAATTGCGGTGCAAGCGTCACTCACAGGTCA
CTTGGTACTCTCAACCCTGCATACCAACACCGCGATTGGTGCGGTGACGCGGCTGCAAGATATGGGGGTTGAGCCGTTTT
TGCTGTCATCGAGCCTCATTGGCGTGGTGGCACAGCGACTAGTGCGCACCTTGTGTCCGCATTGCCATACATGGACGCTT
GCCGATGCCTATCAAACGCAGATTTTTACTGAAATAGGCGAAGTCGGAGAGATTAAACTGCCAAAACCTGTGGGCTGCGA
GAAGTGCAATCAATCAGGATTTCGAGGACGTACCGCGATTTATGAAGTGGTGCCTGTCGATGACAAACTGCGACAACTGA
TTCATAGTCAAACAGCCGAATTTGAGTTAGAAGCGTATGCGCGCAGCAAAACACCATCCATTCGCGCCGATGGACTCAAA
AAAGTATTGGCAGGCAAAACCACACTAGAAGAAGTGTTACGGGTGACAAAAGAAAAAGAGATGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(124-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378Q921

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.912

100

0.439
  pilB Vibrio parahaemolyticus RIMD 2210633

44.398

96.208

0.427
  pilB Vibrio campbellii strain DS40M4

44.05

95.609

0.421
  pilB/pilB1 Synechocystis sp. PCC 6803

39.619

100

0.415
  pilB Legionella pneumophila strain ERS1305867

46.588

84.83

0.395
  pilB Acinetobacter baumannii D1279779

47.132

80.04

0.377
  pilB Acinetobacter baylyi ADP1

45.98

79.441

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.787

77.645

0.363
  pilF Neisseria gonorrhoeae MS11

46.907

77.445

0.363