Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   TP45_RS17545 Genome accession   NZ_CP011250
Coordinates   3855380..3857116 (-) Length   578 a.a.
NCBI ID   WP_007965359.1    Uniprot ID   A0A7Z7J2H4
Organism   Xanthomonas citri pv. aurantifolii strain FDC 1561     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3850380..3862116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TP45_RS17530 (TP45_16725) - 3851594..3853207 (+) 1614 WP_007965357.1 HAMP domain-containing sensor histidine kinase -
  TP45_RS17535 (TP45_16730) pilR 3853477..3854931 (+) 1455 WP_173647341.1 sigma-54 dependent transcriptional regulator Regulator
  TP45_RS17545 (TP45_16735) pilB 3855380..3857116 (-) 1737 WP_007965359.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TP45_RS17550 (TP45_16740) pilA2 3857158..3857571 (-) 414 WP_088411725.1 pilin Machinery gene
  TP45_RS17555 (TP45_16745) comP 3857669..3858097 (-) 429 WP_007962467.1 pilin Machinery gene
  TP45_RS17560 (TP45_16750) pilC 3858443..3859702 (+) 1260 WP_007962468.1 type II secretion system F family protein Machinery gene
  TP45_RS17565 (TP45_16755) - 3859709..3860572 (+) 864 WP_007962469.1 A24 family peptidase -
  TP45_RS17570 (TP45_16760) coaE 3860586..3861197 (+) 612 WP_007962470.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62656.77 Da        Isoelectric Point: 5.7586

>NTDB_id=143844 TP45_RS17545 WP_007965359.1 3855380..3857116(-) (pilB) [Xanthomonas citri pv. aurantifolii strain FDC 1561]
MNVAIGSNLVGITGIARRLVQDGALDEVSARAAMDHATQAKVPLPQWFAEKKLVTAAQLAAANAVEFGMPLMDVSAFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=143844 TP45_RS17545 WP_007965359.1 3855380..3857116(-) (pilB) [Xanthomonas citri pv. aurantifolii strain FDC 1561]
ATGAACGTTGCAATCGGCTCAAATCTAGTTGGAATTACTGGTATTGCGCGGCGCCTTGTTCAGGATGGTGCGCTTGATGA
GGTGAGCGCGCGTGCGGCAATGGATCACGCAACCCAGGCCAAAGTTCCTTTGCCGCAATGGTTCGCCGAAAAGAAGCTTG
TAACAGCCGCACAGCTCGCGGCCGCCAACGCAGTCGAGTTTGGCATGCCTTTGATGGATGTATCGGCATTCGACGCCAAC
CAGAACGCAGTCAAACTGGTCAGTGAGGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAAGCCA
TTCTTGTCGACGAGGATCAGATCCGCCGGACGCTCGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAGGAGATGGGGGATCTGGACGTTTCGGCTGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCGAAGGGTGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGAGGGGCGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAATGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGTGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
TTGTTCCTGGAGGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTACAGCAGAACAACAAGCGTGGCATGACCTTCGCGGCAGCTTTGCGCTCGTTCCTGCGACAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCCCGTTTGATGAACATGGGCATTGCGCCCTACAACATCA
CCTCGTCAGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGGTTCACGCCTGCCCAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAAATCGGCGCGATCGTGC
TGGAGGGCGGCAACGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGAGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z7J2H4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

55.106

98.27

0.542
  pilB Legionella pneumophila strain ERS1305867

51.557

100

0.516
  pilB Vibrio cholerae strain A1552

48.789

100

0.488
  pilF Neisseria gonorrhoeae MS11

49.12

98.27

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

46.429

96.886

0.45
  pilB Vibrio campbellii strain DS40M4

45.167

98.443

0.445

Multiple sequence alignment