Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LG3211_RS18435 Genome accession   NZ_CP011131
Coordinates   4553858..4555588 (+) Length   576 a.a.
NCBI ID   WP_083512649.1    Uniprot ID   -
Organism   Lysobacter gummosus strain 3.2.11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4548858..4560588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG3211_RS18415 (LG3211_4057) - 4550119..4551291 (-) 1173 WP_057944099.1 glycosyltransferase -
  LG3211_RS18420 (LG3211_4058) - 4551288..4552082 (-) 795 WP_148649007.1 hypothetical protein -
  LG3211_RS18425 (LG3211_4059) - 4552138..4552767 (-) 630 WP_057944101.1 class I SAM-dependent methyltransferase -
  LG3211_RS18430 (LG3211_4060) - 4552830..4553723 (-) 894 WP_057944102.1 glycosyltransferase family 2 protein -
  LG3211_RS18435 (LG3211_4061) pilB 4553858..4555588 (+) 1731 WP_083512649.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LG3211_RS18440 (LG3211_4062) pilC 4555593..4556855 (+) 1263 WP_057944103.1 type II secretion system F family protein Machinery gene
  LG3211_RS18445 (LG3211_4063) - 4556877..4557764 (+) 888 WP_057944104.1 A24 family peptidase -
  LG3211_RS18450 (LG3211_4064) coaE 4557767..4558387 (+) 621 WP_057944105.1 dephospho-CoA kinase -
  LG3211_RS18455 (LG3211_4065) - 4558626..4559591 (-) 966 WP_057945583.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62722.05 Da        Isoelectric Point: 6.3660

>NTDB_id=142638 LG3211_RS18435 WP_083512649.1 4553858..4555588(+) (pilB) [Lysobacter gummosus strain 3.2.11]
MSTVATANLVGITGIARRLVMDGALDEVKAREAMTAASAERKPLATYLAEHRLATSAQLAAANSIEFGVPLFDPAAMDPG
QSAIRSISEELIRKHNALPLFKRGNKLFIGLSDPTNTAALDDIKFQTNAAVEAILVDDELIRRSLDRWLESSDQLAAAVG
DGEGLDTLEIGRDDDLANVGADTGIDAKGDDTPVVKFINKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQVAKVPN
KLHARIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRMSTLPTLFGEKIVLRILDGSAARLGIEKLGYEDHQRDL
FVSAVQKPYGMVLVTGPTGSGKTVSLYTALNILNDDQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPEH
ALLAEGFTPEEIAAGVKLYEPVGCPDCTEGYKGRTGIYQVMPMTDEIQAIVLEGGNAMQIAAAAIRSGVRDLRRSALDKA
MNGVTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=142638 LG3211_RS18435 WP_083512649.1 4553858..4555588(+) (pilB) [Lysobacter gummosus strain 3.2.11]
ATGTCCACCGTCGCTACTGCCAATTTGGTGGGGATCACCGGCATCGCCCGGCGTTTAGTCATGGACGGCGCCTTGGACGA
GGTCAAGGCGCGCGAAGCCATGACCGCGGCCAGCGCCGAGCGCAAGCCGCTGGCGACCTACCTGGCCGAACACCGGCTGG
CCACGTCGGCGCAGTTGGCCGCGGCCAACTCGATCGAGTTCGGCGTACCGCTGTTCGACCCCGCCGCGATGGATCCGGGC
CAGTCCGCGATCCGTTCGATCAGCGAAGAGCTGATCCGCAAACATAACGCGCTGCCGCTGTTCAAACGCGGCAACAAGCT
GTTCATCGGCCTGTCCGACCCGACCAACACCGCCGCGCTCGACGACATCAAGTTCCAGACCAACGCCGCCGTCGAGGCGA
TCCTGGTCGATGACGAGCTGATCCGCCGCTCCCTGGACCGCTGGCTGGAGAGCTCCGACCAGCTCGCCGCCGCGGTCGGC
GACGGCGAAGGCCTGGATACGCTGGAGATCGGCCGCGACGACGACCTGGCCAATGTCGGCGCCGACACCGGCATCGACGC
CAAGGGCGACGACACCCCGGTCGTGAAGTTCATCAACAAGGTGTTGGTCGATGCGATCCGCCGCGGCGCCTCCGACATCC
ATTTCGAACCCTACGAAACCGAATACCGCGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGGTCGCCAAGGTGCCCAAC
AAGCTGCATGCGCGCATCGCCGCGCGCCTGAAGGTCATGGCGCAGCTCGACATCGCCGAGAAGCGCGTGCCGCAGGACGG
ACGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTCCGCATGAGCACCTTGCCGACCCTGTTCGGCGAGAAGA
TCGTGCTGCGTATCCTCGACGGCAGCGCGGCGCGGCTGGGTATCGAGAAGCTCGGCTACGAGGACCACCAGCGCGATCTG
TTCGTCAGTGCGGTGCAAAAGCCTTACGGCATGGTCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTA
CACCGCGCTCAACATCCTCAACGACGACCAACGCAACATCTCCACGGTCGAAGACCCGGTCGAAATCCGCGTGCCCGGCA
TCAACCAGGTACAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCGGCCCTGCGCAGCTTCCTGCGTCAGGATCCGGAC
GTGATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTGCT
GTCCACCCTGCACACCAACGACGCCCCGCAGACCATCGCGCGCCTGATGAACATGGGCGTGGCGCCGTACAACATCACCT
CCTCGGTCACCCTGGTGATCGCCCAGCGCCTGGCCCGCCGCCTGCACGACTGCAAGCGCGAGGTGCATCTGCCCGAGCAC
GCCCTGCTCGCCGAAGGCTTCACCCCCGAAGAAATTGCCGCCGGCGTCAAACTCTATGAGCCGGTCGGGTGCCCGGATTG
CACCGAAGGCTACAAAGGACGTACCGGCATCTATCAGGTCATGCCCATGACCGACGAAATCCAGGCCATCGTGCTCGAAG
GCGGCAATGCGATGCAGATCGCCGCCGCCGCCATCCGGTCGGGCGTGCGCGATCTGCGCCGCTCGGCCTTGGACAAGGCG
ATGAACGGTGTCACCAGCCTGGCCGAAATCAACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.85

98.438

0.54
  pilB Acinetobacter baumannii D1279779

54.482

98.785

0.538
  pilB Legionella pneumophila strain ERS1305867

50.439

98.785

0.498
  pilB Vibrio cholerae strain A1552

48.951

99.306

0.486
  pilF Neisseria gonorrhoeae MS11

47.285

99.132

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

49.331

90.799

0.448
  pilB Vibrio campbellii strain DS40M4

51.633

85.069

0.439
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.364

100

0.375

Multiple sequence alignment