Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PISS_RS13715 Genome accession   NZ_CP011030
Coordinates   2959552..2961228 (+) Length   558 a.a.
NCBI ID   WP_024601729.1    Uniprot ID   -
Organism   Pseudoalteromonas issachenkonii strain KMM 3549     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2954552..2966228
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PISS_RS13690 (PISS_a3067) nadC 2954587..2955432 (+) 846 WP_058155275.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PISS_RS13695 (PISS_a3068) - 2955940..2956374 (+) 435 WP_157066903.1 pilin -
  PISS_RS13700 (PISS_a3070) pilC 2956448..2957677 (+) 1230 WP_058155276.1 type II secretion system F family protein Machinery gene
  PISS_RS13705 (PISS_a3071) pilD 2957875..2958786 (+) 912 WP_058155277.1 A24 family peptidase Machinery gene
  PISS_RS13710 (PISS_a3072) coaE 2958789..2959418 (+) 630 WP_058155278.1 dephospho-CoA kinase -
  PISS_RS13715 (PISS_a3073) pilB 2959552..2961228 (+) 1677 WP_024601729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PISS_RS13720 (PISS_a3074) yacG 2961293..2961523 (+) 231 WP_024601728.1 DNA gyrase inhibitor YacG -
  PISS_RS13725 (PISS_a3076) - 2961583..2962071 (-) 489 WP_036956691.1 Spy/CpxP family protein refolding chaperone -
  PISS_RS13730 (PISS_a3078) - 2962239..2962928 (+) 690 WP_016899750.1 response regulator transcription factor -
  PISS_RS13735 (PISS_a3080) - 2962906..2964333 (+) 1428 WP_036956692.1 ATP-binding protein -
  PISS_RS13740 (PISS_a3081) - 2964323..2965267 (+) 945 WP_036956693.1 alpha/beta fold hydrolase -
  PISS_RS13745 (PISS_a3082) - 2965264..2966094 (-) 831 WP_083494667.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62402.69 Da        Isoelectric Point: 6.1023

>NTDB_id=141599 PISS_RS13715 WP_024601729.1 2959552..2961228(+) (pilB) [Pseudoalteromonas issachenkonii strain KMM 3549]
MNINSPLLRKFITLGRVDTDTVKTRQQDFASTAELISKCGKIDSKDLAEQCIDLFRVPFFDLKDFNVAQISAELVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYVQLDNKIEQLLDATGSLNLSEDEFKEFAELDSEQ
HKEHTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLASKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCSKCKTPEQLPREELIRQGFSEQQIQDMVL
YAPKGCENCTDGYKGRVGIYEVMKITPEIAQIIMRGGNSLEIAEVSLKAGYNNLRLSGIRKAAEGVTSLAEINRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=141599 PISS_RS13715 WP_024601729.1 2959552..2961228(+) (pilB) [Pseudoalteromonas issachenkonii strain KMM 3549]
ATGAATATTAACTCACCGTTATTAAGAAAATTTATTACCCTCGGCCGGGTTGATACTGATACCGTAAAAACACGGCAGCA
AGATTTTGCCTCAACTGCAGAGCTTATCTCTAAATGTGGAAAAATAGACAGTAAAGATTTAGCTGAGCAATGCATTGATT
TATTTAGAGTGCCTTTTTTTGATTTAAAAGATTTTAATGTTGCACAAATATCAGCTGAGCTGGTTAAAGAAAAACTGATC
CGCAAACACCATATTTTGCCTTTGGTACAAAAAGGCAGAAAAGTGTATATTGCCGCCTCAGATCCTACCGATTATGGTGC
CTTTGAAAACTTTGAATTTAGCACGGGTTTATCGTGCGAAGTAGTTGTGGTTGACTACGTCCAGCTCGATAACAAAATTG
AGCAATTACTCGATGCCACTGGCAGTTTAAATTTAAGTGAAGACGAATTTAAAGAGTTCGCTGAACTCGATTCTGAGCAG
CACAAAGAACACACTCCTAAAGACGATGACAAAGACGACGCCCCGATAATTGTCTACATTAATAAAATTTTAATGGATGC
AATAAAAAAAGGCGCCTCAGATTTACACTTTGAACCTTATGAACACAAATACCGTATTCGCTTTCGCATTGATGGTATTT
TGCACGAAGTAGCCAGCCCGCCAAATACATTGGCCAGTAAGCTATCGGCGCGAATAAAAGTAATGTCGCGACTGGATATT
GCAGAAAAGCGTAAACCACAAGATGGCCGTATTAAGCTTAAGATAACAGAGCGTAAAAGCATTGATTTTCGTGTTAGTAC
CATGCCAACTTTGTGGGGCGAAAAAATTGTAATGCGTATTCTTGATTCATCCAGTGCCATGCTAGGTATTGATGTATTAG
GCTATGAACCCGAGCAGAAAAAACTATACCTCGATGCACTTGAGCAACCGCAAGGAATGATTTTGGTTACCGGCCCAACT
GGTTCAGGTAAAACGGTTTCTTTGTATACGGGCTTAAATATACTCAATCAGCCGGAGCGGAATATTAGTACCGCCGAAGA
TCCGGTAGAAATTAACCTCGAAGGGGTTAACCAAGTACAAATAAATACTAAAGCAGATATGACTTTTGCTAATGCACTGC
GGGCGTTTTTACGTCAAGATCCCGATGTGGTGATGGTGGGTGAAATACGTGATTTAGAAACTGCAGAAATATCAATTAAA
GCGGCGCAAACAGGTCACTTGGTGCTGTCAACACTGCATACCAACTCAGCGCCAGAAACGATTACTCGTTTGTTAAATAT
GGGTGTACCCGCGTATAATGTGGCCAGTTCTATTAGTTTAATTATAGCTCAGCGTTTAGCCCGAAGGTTATGCTCTAAAT
GTAAAACACCAGAGCAGTTACCTCGCGAAGAGCTTATACGCCAAGGCTTTAGTGAACAACAAATTCAAGACATGGTTTTA
TACGCACCTAAAGGCTGTGAAAACTGTACCGATGGCTACAAAGGCCGTGTTGGTATTTACGAAGTAATGAAAATTACGCC
TGAAATAGCGCAAATTATAATGCGCGGCGGTAACTCATTAGAAATTGCTGAGGTATCACTTAAAGCAGGATATAACAATT
TGCGTTTATCGGGTATTCGTAAAGCCGCAGAGGGTGTGACCTCACTTGCTGAGATTAATCGTGTCACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.593

96.774

0.557
  pilB Acinetobacter baylyi ADP1

58.416

90.502

0.529
  pilB Legionella pneumophila strain ERS1305867

55.273

91.756

0.507
  pilB Vibrio cholerae strain A1552

52.06

95.699

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

51.685

95.699

0.495
  pilB Vibrio campbellii strain DS40M4

51.124

95.699

0.489
  pilF Neisseria gonorrhoeae MS11

49.808

93.548

0.466
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.382

92.832

0.366

Multiple sequence alignment