Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   TQ33_RS01735 Genome accession   NZ_CP010975
Coordinates   366275..367342 (+) Length   355 a.a.
NCBI ID   WP_046560535.1    Uniprot ID   -
Organism   Kangiella geojedonensis strain YCS-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 361275..372342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TQ33_RS01725 (TQ33_0348) rpmE 363125..363337 (+) 213 WP_046560534.1 50S ribosomal protein L31 -
  TQ33_RS01730 (TQ33_0349) - 363456..366020 (-) 2565 WP_052735160.1 penicillin-binding protein 1A -
  TQ33_RS01735 (TQ33_0350) pilM 366275..367342 (+) 1068 WP_046560535.1 pilus assembly protein PilM Machinery gene
  TQ33_RS01740 (TQ33_0351) - 367342..367938 (+) 597 WP_046560536.1 PilN domain-containing protein -
  TQ33_RS01745 (TQ33_0352) - 367938..368549 (+) 612 WP_046560537.1 type 4a pilus biogenesis protein PilO -
  TQ33_RS01750 (TQ33_0353) - 368552..369109 (+) 558 WP_228640316.1 pilus assembly protein PilP -
  TQ33_RS01755 (TQ33_0354) pilQ 369120..371282 (+) 2163 WP_046560539.1 type IV pilus secretin PilQ -
  TQ33_RS01760 (TQ33_0355) aroK 371377..371901 (+) 525 WP_046560540.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 38365.48 Da        Isoelectric Point: 4.3329

>NTDB_id=140995 TQ33_RS01735 WP_046560535.1 366275..367342(+) (pilM) [Kangiella geojedonensis strain YCS-5]
MVLGLFKNKQVPVIGIDISSTAVKVLQLGKSGGRYRVDRYAVEPLPQGAVVERDIRDTEAVGNAIKKAVQKANAKTKDAA
VAVSGSAVITRSIQMEKGLSDQEMEDVIKVEADQYIPYPLEEVNLDFEVLGDSANDDNLVDVLLAASRSVNVDTRVDALD
IAGLSPKIVDIEAYAIERAYQLTKKKTVEHLEDSVVAIIDIGATMTSLNVLQNGQVIYNREQLFGGNQLTEEIQSRYGLS
YQEAGLAKKQGSLPDDYETEVLMPFAEIITQQVGRALQFFFSASEFSSVNEVILAGGCASIDGLDSMIQEHLGTPTRVAN
PFADMSISSHVNTQALSADAPAMMISCGLALRSFD

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=140995 TQ33_RS01735 WP_046560535.1 366275..367342(+) (pilM) [Kangiella geojedonensis strain YCS-5]
ATGGTTCTGGGGTTATTCAAAAATAAACAAGTACCAGTGATAGGTATTGATATCAGCTCGACTGCAGTTAAAGTCTTACA
GCTTGGTAAGTCAGGTGGTCGATACAGAGTTGATAGATATGCTGTCGAACCATTACCGCAAGGCGCTGTTGTTGAACGTG
ATATTCGTGATACCGAAGCGGTTGGTAATGCAATAAAGAAAGCTGTTCAAAAAGCGAACGCAAAAACAAAAGATGCCGCT
GTTGCAGTGTCTGGCTCCGCAGTGATTACTCGCTCCATACAAATGGAGAAAGGCCTTTCTGATCAAGAAATGGAAGATGT
CATCAAAGTTGAAGCCGATCAATACATTCCATACCCTCTTGAAGAGGTGAACCTTGATTTCGAGGTGTTAGGGGATTCTG
CGAATGACGATAACTTAGTTGACGTCTTATTGGCCGCATCTCGTAGTGTGAATGTGGATACGCGTGTTGATGCTTTGGAT
ATTGCCGGCTTATCACCAAAAATCGTTGATATCGAAGCCTATGCCATTGAGCGTGCTTATCAATTGACGAAAAAGAAGAC
GGTTGAGCACCTTGAAGATTCTGTGGTGGCGATAATTGATATCGGCGCAACGATGACCAGTCTCAATGTACTGCAAAACG
GACAAGTTATTTATAACCGTGAGCAGTTATTCGGTGGCAACCAGCTAACCGAAGAGATTCAGAGTCGATATGGACTGTCG
TACCAAGAAGCCGGACTTGCGAAGAAGCAAGGTAGTTTGCCGGATGATTATGAAACTGAAGTACTGATGCCTTTTGCTGA
GATTATTACGCAGCAAGTTGGGCGCGCTCTGCAGTTCTTCTTCTCTGCCAGTGAGTTCAGCTCAGTAAATGAAGTGATTC
TTGCAGGTGGCTGTGCCTCGATTGATGGCCTGGATAGCATGATTCAAGAGCACTTAGGTACTCCGACTCGTGTGGCTAAC
CCTTTTGCCGATATGTCTATCTCATCTCATGTGAACACACAAGCATTGAGCGCGGATGCGCCAGCAATGATGATAAGCTG
TGGACTAGCATTAAGGAGTTTTGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(15-354)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.282

99.718

0.501
  comM Acinetobacter nosocomialis M2

50

99.718

0.499
  pilM Legionella pneumophila strain ERS1305867

46.893

99.718

0.468
  comM Acinetobacter baylyi ADP1

46.328

99.718

0.462

Multiple sequence alignment