Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   ST12_RS15990 Genome accession   NZ_CP010521
Coordinates   3595889..3596332 (-) Length   147 a.a.
NCBI ID   WP_003369942.1    Uniprot ID   A0A0C2N5G6
Organism   Clostridium botulinum strain NCTC 8550     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 3564328..3602038 3595889..3596332 within 0


Gene organization within MGE regions


Location: 3564328..3602038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST12_RS15780 (ST12_15780) bilQ 3564328..3564765 (-) 438 WP_012451029.1 bilirubin utilization transcriptional regulator BilQ -
  ST12_RS15785 (ST12_15785) - 3564977..3566275 (-) 1299 WP_012449689.1 DEAD/DEAH box helicase -
  ST12_RS15790 (ST12_15790) - 3566606..3566758 (-) 153 WP_003373161.1 zinc-ribbon domain-containing protein -
  ST12_RS15795 (ST12_15795) - 3567030..3567536 (-) 507 WP_012450318.1 DUF2798 domain-containing protein -
  ST12_RS15800 (ST12_15800) rlmH 3567699..3568178 (-) 480 WP_012450724.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  ST12_RS15805 (ST12_15805) - 3568468..3569109 (-) 642 WP_040968354.1 lactate utilization protein -
  ST12_RS15810 (ST12_15810) - 3569483..3569980 (-) 498 WP_003374022.1 SEC-C metal-binding domain-containing protein -
  ST12_RS15815 (ST12_15815) - 3570043..3570834 (-) 792 WP_012450919.1 MBL fold metallo-hydrolase -
  ST12_RS15820 (ST12_15820) - 3570869..3572131 (-) 1263 WP_003370381.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  ST12_RS15825 (ST12_15825) - 3572525..3573064 (+) 540 WP_012449467.1 hypothetical protein -
  ST12_RS15830 (ST12_15830) - 3573241..3573717 (+) 477 WP_003373384.1 dUTP diphosphatase -
  ST12_RS15835 (ST12_15835) - 3573907..3574338 (+) 432 WP_003369607.1 DMT family transporter -
  ST12_RS15840 (ST12_15840) - 3574396..3575214 (+) 819 WP_012450283.1 MBL fold metallo-hydrolase -
  ST12_RS15845 (ST12_15845) - 3575348..3576553 (+) 1206 WP_012450935.1 S8 family serine peptidase -
  ST12_RS15850 (ST12_15850) - 3576663..3577847 (-) 1185 WP_017825380.1 M23 family metallopeptidase -
  ST12_RS15855 (ST12_15855) - 3578023..3579012 (+) 990 WP_012449871.1 DnaD domain protein -
  ST12_RS15860 (ST12_15860) - 3579005..3579985 (+) 981 WP_012450454.1 ATP-binding protein -
  ST12_RS15920 (ST12_15920) - 3581186..3581770 (-) 585 WP_012451320.1 hypothetical protein -
  ST12_RS15925 (ST12_15925) - 3581934..3583178 (+) 1245 WP_012449819.1 NAD(P)/FAD-dependent oxidoreductase -
  ST12_RS15930 (ST12_15930) - 3583348..3584097 (-) 750 WP_012450489.1 acyl-ACP thioesterase domain-containing protein -
  ST12_RS15935 (ST12_15935) - 3584304..3584486 (-) 183 WP_003371961.1 DUF1858 domain-containing protein -
  ST12_RS15940 (ST12_15940) - 3584589..3585875 (-) 1287 WP_012451558.1 adenylosuccinate synthase -
  ST12_RS15945 (ST12_15945) - 3586099..3587271 (+) 1173 WP_012450311.1 malic enzyme-like NAD(P)-binding protein -
  ST12_RS15950 (ST12_15950) proC 3587972..3588772 (-) 801 WP_012451666.1 pyrroline-5-carboxylate reductase -
  ST12_RS15955 (ST12_15955) - 3588987..3589340 (+) 354 WP_012450834.1 hypothetical protein -
  ST12_RS15960 (ST12_15960) - 3589362..3590702 (-) 1341 WP_003368893.1 replicative DNA helicase -
  ST12_RS15965 (ST12_15965) lonC 3590789..3592681 (-) 1893 WP_012450022.1 Lon family ATP-dependent protease -
  ST12_RS15970 (ST12_15970) rplI 3592697..3593140 (-) 444 WP_012425064.1 50S ribosomal protein L9 -
  ST12_RS15975 (ST12_15975) - 3593143..3595083 (-) 1941 WP_003374727.1 DHH family phosphoesterase -
  ST12_RS15980 (ST12_15980) - 3595108..3595428 (-) 321 WP_003374679.1 MazG-like family protein -
  ST12_RS15985 (ST12_15985) rpsR 3595616..3595867 (-) 252 WP_003369777.1 30S ribosomal protein S18 -
  ST12_RS15990 (ST12_15990) ssbA 3595889..3596332 (-) 444 WP_003369942.1 single-stranded DNA-binding protein Machinery gene
  ST12_RS15995 (ST12_15995) rpsF 3596346..3596633 (-) 288 WP_026072100.1 30S ribosomal protein S6 -
  ST12_RS16000 (ST12_16000) - 3596743..3596934 (-) 192 WP_003373736.1 DUF951 domain-containing protein -
  ST12_RS16005 (ST12_16005) - 3596934..3597827 (-) 894 WP_012451521.1 mechanosensitive ion channel family protein -
  ST12_RS16010 (ST12_16010) - 3597855..3598094 (-) 240 WP_012450983.1 DUF3343 domain-containing protein -
  ST12_RS16015 (ST12_16015) - 3598496..3599149 (+) 654 WP_012451282.1 LysE family transporter -
  ST12_RS16020 (ST12_16020) - 3599165..3600322 (+) 1158 WP_012449527.1 aminotransferase class V-fold PLP-dependent enzyme -
  ST12_RS16025 (ST12_16025) yyaC 3600621..3601223 (+) 603 WP_003369076.1 spore protease YyaC -
  ST12_RS16030 (ST12_16030) - 3601520..3602038 (-) 519 WP_003374247.1 DUF4446 family protein -

Sequence


Protein


Download         Length: 147 a.a.        Molecular weight: 16065.78 Da        Isoelectric Point: 4.5882

>NTDB_id=138904 ST12_RS15990 WP_003369942.1 3595889..3596332(-) (ssbA) [Clostridium botulinum strain NCTC 8550]
MNKVVLIGRLTKDPELRFTPGAGTAVTTLTLAVDKYNSKSGQKEADFVPVVVWGKQAESTANYMSKGSQMAISGRIQTRN
YEAKDGTKRYVTEVVATEVQFLSKSNASGNVGTNYGNNTEYSSSNNPFDDMNFEEDITPVDDGDMPF

Nucleotide


Download         Length: 444 bp        

>NTDB_id=138904 ST12_RS15990 WP_003369942.1 3595889..3596332(-) (ssbA) [Clostridium botulinum strain NCTC 8550]
ATGAACAAAGTAGTTTTAATCGGAAGATTGACGAAAGATCCAGAACTAAGATTTACTCCTGGAGCTGGAACAGCAGTAAC
TACCTTAACATTAGCAGTTGATAAGTATAATTCTAAATCTGGTCAAAAAGAAGCGGACTTTGTACCTGTAGTTGTATGGG
GAAAACAAGCTGAAAGTACTGCGAATTACATGAGTAAGGGTAGCCAAATGGCTATAAGCGGTAGAATTCAAACCAGAAAT
TATGAAGCAAAAGATGGAACAAAGAGATATGTTACAGAAGTAGTTGCTACAGAAGTTCAATTTTTAAGTAAATCAAATGC
TTCAGGAAATGTTGGTACTAATTATGGTAACAACACTGAATATTCATCATCAAATAATCCATTTGATGATATGAACTTTG
AAGAAGACATAACTCCAGTAGATGATGGAGATATGCCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0C2N5G6

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

43.605

100

0.51

  ssbB Streptococcus sobrinus strain NIDR 6715-7

43.165

94.558

0.408

  ssbA Streptococcus mutans UA159

43.165

94.558

0.408

  ssb Latilactobacillus sakei subsp. sakei 23K

40.876

93.197

0.381

  ssbB Bacillus subtilis subsp. subtilis str. 168

53.398

70.068

0.374


Multiple sequence alignment