Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AKK22_RS21685 Genome accession   NZ_CP013029
Coordinates   4040889..4042376 (-) Length   495 a.a.
NCBI ID   WP_032205960.1    Uniprot ID   -
Organism   Escherichia coli strain 2012C-4227     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4035889..4047376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKK22_RS32260 - 4036949..4037017 (+) 69 Protein_4075 type IV toxin-antitoxin system YeeU family antitoxin -
  AKK22_RS21650 (AKK22_21160) - 4037064..4037441 (+) 378 WP_000854916.1 TA system toxin CbtA family protein -
  AKK22_RS21655 (AKK22_21165) - 4037438..4037698 (+) 261 Protein_4077 DUF5983 family protein -
  AKK22_RS21665 (AKK22_21175) - 4037753..4038966 (+) 1214 WP_407059857.1 IS3 family transposase -
  AKK22_RS21670 (AKK22_21180) - 4039008..4039190 (-) 183 Protein_4079 type II secretion system protein -
  AKK22_RS21675 (AKK22_21185) gspG 4039194..4039649 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  AKK22_RS21680 (AKK22_21190) gspF 4039666..4040889 (-) 1224 WP_032205959.1 type II secretion system inner membrane protein GspF -
  AKK22_RS21685 (AKK22_21195) pilB/pilB1 4040889..4042376 (-) 1488 WP_032205960.1 type II secretion system ATPase GspE Machinery gene
  AKK22_RS21690 (AKK22_21200) gspD 4042376..4044436 (-) 2061 Protein_4083 type II secretion system secretin GspD -
  AKK22_RS21695 (AKK22_21205) gspC 4044466..4045425 (-) 960 WP_032205961.1 type II secretion system protein GspC -
  AKK22_RS21700 (AKK22_21210) gspS2 4045443..4045853 (-) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  AKK22_RS21705 (AKK22_21215) pilD 4045919..4046728 (-) 810 WP_032205962.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 495 a.a.        Molecular weight: 54933.09 Da        Isoelectric Point: 6.3957

>NTDB_id=138460 AKK22_RS21685 WP_032205960.1 4040889..4042376(-) (pilB/pilB1) [Escherichia coli strain 2012C-4227]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQERQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEVASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGALERYVRQHSAGIRSNGIEKVLA
GEASLDEVLRVTMEA

Nucleotide


Download         Length: 1488 bp        

>NTDB_id=138460 AKK22_RS21685 WP_032205960.1 4040889..4042376(-) (pilB/pilB1) [Escherichia coli strain 2012C-4227]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCGGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCGGAAGAACTGCCAGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGTCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGCCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCATCGCTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGTCCCGACTGCCGTCAGGCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGCATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTCCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGCGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTGCTCGCC
GGAGAAGCCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.114

100

0.428
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.393

85.253

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

47.607

80.202

0.382
  pilB Legionella pneumophila strain ERS1305867

43.619

87.071

0.38
  pilB Acinetobacter baumannii D1279779

46.366

80.606

0.374
  pilB Vibrio campbellii strain DS40M4

46.465

80

0.372
  pilB Vibrio cholerae strain A1552

45.96

80

0.368
  pilB Acinetobacter baylyi ADP1

46.41

78.788

0.366
  pilF Thermus thermophilus HB27

44.75

80.808

0.362

Multiple sequence alignment