Detailed information NTG0137306

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AOY09_RS31110
• Genome accession: NZ_CP012901
• Coordinates: 6465788..6466936 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain N15-01092
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6460788..6471936

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AOY09_RS31090 (AOY09_06054)	-	6461525..6462709 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
AOY09_RS31095 (AOY09_06055)	-	6462706..6464079 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
AOY09_RS31100 (AOY09_06056)	-	6464322..6464726 (-)	405	WP_003084560.1	NINE protein	-
AOY09_RS31105 (AOY09_06057)	czcD	6464881..6465780 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
AOY09_RS31110 (AOY09_06058)	pilU	6465788..6466936 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
AOY09_RS31115 (AOY09_06059)	pilT	6467114..6468148 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
AOY09_RS31120 (AOY09_06060)	-	6468363..6469055 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
AOY09_RS31125 (AOY09_06061)	proC	6469067..6469888 (+)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
AOY09_RS31130 (AOY09_06062)	-	6469899..6470492 (+)	594	WP_003084544.1	YggT family protein	-
AOY09_RS31135	-	6470489..6470785 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=137306 AOY09_RS31110 WP_003100959.1 6465788..6466936(-) (pilU) [Pseudomonas aeruginosa strain N15-01092]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=137306 AOY09_RS31110 WP_003100959.1 6465788..6466936(-) (pilU) [Pseudomonas aeruginosa strain N15-01092]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382