Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   RR32_RS01610 Genome accession   NZ_CP010368
Coordinates   347995..348735 (+) Length   246 a.a.
NCBI ID   WP_006581061.1    Uniprot ID   A0A8B4N9D4
Organism   Acinetobacter nosocomialis strain 6411     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 342995..353735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RR32_RS01595 (RR32_01600) ponA 343576..346134 (-) 2559 WP_039246126.1 penicillin-binding protein PBP1a -
  RR32_RS01600 (RR32_01605) comM 346296..347354 (+) 1059 WP_004711654.1 pilus assembly protein PilM Machinery gene
  RR32_RS01605 (RR32_01610) pilN 347354..347998 (+) 645 WP_004711656.1 PilN domain-containing protein Machinery gene
  RR32_RS01610 (RR32_01615) pilO 347995..348735 (+) 741 WP_006581061.1 type 4a pilus biogenesis protein PilO Machinery gene
  RR32_RS01615 (RR32_01620) pilP 348746..349273 (+) 528 WP_004711660.1 pilus assembly protein PilP Machinery gene
  RR32_RS01620 (RR32_01625) pilQ 349336..351501 (+) 2166 WP_039246129.1 type IV pilus secretin PilQ Machinery gene
  RR32_RS01625 (RR32_01630) aroK 351513..352082 (+) 570 WP_002051689.1 shikimate kinase AroK -
  RR32_RS01630 (RR32_01635) aroB 352111..353193 (+) 1083 WP_039246131.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27611.37 Da        Isoelectric Point: 4.9673

>NTDB_id=136605 RR32_RS01610 WP_006581061.1 347995..348735(+) (pilO) [Acinetobacter nosocomialis strain 6411]
MSQDELQELSLEQSASKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
GNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSISALPRIVTMHDFVVEAAQAKDSKSDIPVLNYSIKAKTYRYIGATEASAQPAKAAAATAPTS
PNAQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=136605 RR32_RS01610 WP_006581061.1 347995..348735(+) (pilO) [Acinetobacter nosocomialis strain 6411]
ATGAGTCAAGATGAACTGCAAGAATTATCATTAGAGCAATCAGCTTCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTTGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACTTGCTGGATTTTTATTT
TCTTTGCTGTATTGGCTTTGGGTTATTTTGTTGCTATTCAGCCAAAGCTACAAGCAATTGATAATGCCCAAGCTCAGGAA
GGTAATTTATTAAATGAATTTCGAGAAAAAGACTCCAAATTACGTAACCTTCAGCAGTATCAAGTGCAGCTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAATTAGAACAGTTGCCGAAAGAAACAGAGATTCCAAGTTTAGTTGAGGATATTAACTTAA
CTGGTGTGAACTCTGGATTGAAATTTAAAAATATTCGTTTAGAGGATGAAGTAAAGCAGGAAATTTTTATTGAACAACCA
ATTGCCATCGAAGCAACAGGCGACTATCACGCTTTCGGTGCTTTCGTAAGTAGTATTTCTGCTTTGCCACGTATTGTGAC
GATGCATGACTTTGTTGTAGAGGCTGCTCAAGCTAAAGATAGTAAGTCAGATATTCCTGTGCTTAATTATTCTATTAAAG
CCAAAACCTATCGATATATAGGAGCAACAGAGGCATCGGCACAACCAGCTAAGGCTGCAGCGGCGACTGCACCAACTAGC
CCTAATGCTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B4N9D4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

89.431

100

0.894
  comO Acinetobacter baylyi ADP1

70.954

97.967

0.695

Multiple sequence alignment