Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AN205_RS02060 Genome accession   NZ_CP012631
Coordinates   429383..430864 (-) Length   493 a.a.
NCBI ID   WP_001219891.1    Uniprot ID   B7MCU1
Organism   Escherichia coli strain SF-173     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 424383..435864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AN205_RS02030 (AN205_02035) gspK 425287..426261 (-) 975 WP_042016647.1 type II secretion system minor pseudopilin GspK -
  AN205_RS02035 (AN205_02040) gspJ 426254..426841 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  AN205_RS02040 (AN205_02045) gspI 426834..427229 (-) 396 WP_001041747.1 type II secretion system minor pseudopilin GspI -
  AN205_RS02045 (AN205_02050) gspH 427226..427735 (-) 510 WP_001076038.1 type II secretion system minor pseudopilin GspH -
  AN205_RS27630 (AN205_02055) gspG 427743..428180 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  AN205_RS02055 (AN205_02060) gspF 428190..429386 (-) 1197 WP_001109576.1 type II secretion system inner membrane protein GspF -
  AN205_RS02060 (AN205_02065) pilB 429383..430864 (-) 1482 WP_001219891.1 type II secretion system ATPase GspE Machinery gene
  AN205_RS02065 (AN205_02070) gspD 430874..432826 (-) 1953 WP_001514015.1 type II secretion system secretin GspD -
  AN205_RS02070 (AN205_02075) gspC 432810..433625 (-) 816 WP_001142674.1 type II secretion system protein GspC -
  AN205_RS02075 (AN205_02080) gspA 433805..435274 (+) 1470 WP_000107586.1 type II secretion system protein GspA -
  AN205_RS02080 (AN205_02085) gspB 435276..435695 (+) 420 WP_000461443.1 putative general secretion pathway protein GspB -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54666.59 Da        Isoelectric Point: 6.3224

>NTDB_id=135045 AN205_RS02060 WP_001219891.1 429383..430864(-) (pilB) [Escherichia coli strain SF-173]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
HHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
VMRVASATLENEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=135045 AN205_RS02060 WP_001219891.1 429383..430864(-) (pilB) [Escherichia coli strain SF-173]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAACGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGGCTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAAGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCTATTTTGAGTGAGGCCATCAAAGAAACCGCCTCCGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGACGTATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTGCTCCGTCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAACAGGATTTAGAAAATCTCATCCAGCTT
CCACACGGTATTATCCTGGTGACCGGACCGACAGGCTCCGGTAAAAGTACCACGCTCTACGCCATTCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAACTGGAAGGCATAGGACAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAAATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCACTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGTCTGTGTCCGCAATGCCGACAATTCACGCCTGTATCGCCGCAACAAGCGCAGATGTTTAAA
CATCATCAGCTCGCGGTGACGACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTACCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTGCGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTGGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATACGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAACGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7MCU1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.697

89.047

0.416
  pilB Vibrio cholerae strain A1552

48.441

84.584

0.41
  pilB Acinetobacter baumannii D1279779

45.682

89.249

0.408
  pilB Legionella pneumophila strain ERS1305867

41.372

97.566

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

46.588

86.207

0.402
  pilB/pilB1 Synechocystis sp. PCC 6803

41.202

94.523

0.389
  pilB Acinetobacter baylyi ADP1

48.593

79.31

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.178

79.919

0.361
  pilF Thermus thermophilus HB27

45.063

80.122

0.361