Detailed information NTG0133166

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: OY17_RS15205
• Genome accession: NZ_CP009938
• Coordinates: 3154443..3155057 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus sp. BH072 isolate honey
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3149443..3160057

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OY17_RS15180 (OY17_15180)	rpsT	3149746..3150012 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
OY17_RS15185 (OY17_15185)	holA	3150029..3151070 (-)	1042	Protein_3011	DNA polymerase III subunit delta	-
OY17_RS20555	-	3151110..3151261 (-)	152	Protein_3012	hypothetical protein	-
OY17_RS20195 (OY17_15190)	-	3151302..3151436 (+)	135	WP_003152870.1	YqzM family protein	-
OY17_RS15195 (OY17_15195)	comEC	3151455..3153806 (-)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
OY17_RS15200 (OY17_15200)	-	3153807..3154376 (-)	570	WP_003152868.1	ComE operon protein 2	-
OY17_RS15205 (OY17_15205)	comEA	3154443..3155057 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
OY17_RS15210 (OY17_15210)	comER	3155116..3155937 (+)	822	WP_014418420.1	late competence protein ComER	-
OY17_RS15215 (OY17_15215)	-	3156006..3156743 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
OY17_RS15220 (OY17_15220)	rsfS	3156740..3157096 (-)	357	WP_007408260.1	ribosome silencing factor	-
OY17_RS15225 (OY17_15225)	yqeK	3157114..3157674 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
OY17_RS15230 (OY17_15230)	-	3157664..3158233 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
OY17_RS15235 (OY17_15235)	yhbY	3158244..3158534 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
OY17_RS15240 (OY17_15240)	aroE	3158528..3159370 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=133166 OY17_RS15205 WP_014418419.1 3154443..3155057(-) (comEA) [Bacillus sp. BH072 isolate honey]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=133166 OY17_RS15205 WP_014418419.1 3154443..3155057(-) (comEA) [Bacillus sp. BH072 isolate honey]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363