Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   BG04_RS11365 Genome accession   NZ_CP009920
Coordinates   2105336..2105836 (-) Length   166 a.a.
NCBI ID   WP_013059914.1    Uniprot ID   A0A0B6A5N1
Organism   Priestia megaterium NBRC 15308 = ATCC 14581     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2092209..2148495 2105336..2105836 within 0


Gene organization within MGE regions


Location: 2092209..2148495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG04_RS11290 (BG04_2245) - 2092209..2093003 (-) 795 WP_013059903.1 MBL fold metallo-hydrolase -
  BG04_RS11295 (BG04_2246) - 2093022..2093801 (-) 780 WP_016765985.1 two-component system regulatory protein YycI -
  BG04_RS11300 (BG04_2247) - 2093788..2095155 (-) 1368 WP_028409756.1 YycH family regulatory protein -
  BG04_RS11305 (BG04_2248) walK 2095146..2097053 (-) 1908 WP_013085551.1 cell wall metabolism sensor histidine kinase WalK -
  BG04_RS11310 (BG04_2249) yycF 2097060..2097767 (-) 708 WP_013085552.1 response regulator YycF -
  BG04_RS11335 (BG04_2254) - 2098537..2099823 (-) 1287 WP_013085553.1 adenylosuccinate synthase -
  BG04_RS11340 (BG04_2255) dnaB 2100011..2101375 (-) 1365 WP_013059909.1 replicative DNA helicase -
  BG04_RS11345 (BG04_2256) rplI 2101491..2101940 (-) 450 WP_013085555.1 50S ribosomal protein L9 -
  BG04_RS11350 (BG04_2257) - 2101937..2103910 (-) 1974 WP_034648181.1 DHH family phosphoesterase -
  BG04_RS11355 (BG04_2258) - 2103947..2104891 (-) 945 WP_034648180.1 YybS family protein -
  BG04_RS11360 (BG04_2259) rpsR 2105030..2105269 (-) 240 WP_013059913.1 30S ribosomal protein S18 -
  BG04_RS11365 (BG04_2260) ssbA 2105336..2105836 (-) 501 WP_013059914.1 single-stranded DNA-binding protein Machinery gene
  BG04_RS11370 (BG04_2261) rpsF 2105885..2106172 (-) 288 WP_013059915.1 30S ribosomal protein S6 -
  BG04_RS11375 (BG04_2262) ychF 2106362..2107462 (-) 1101 WP_013059916.1 redox-regulated ATPase YchF -
  BG04_RS11380 (BG04_2263) - 2107631..2107828 (-) 198 WP_013059917.1 DUF951 domain-containing protein -
  BG04_RS11385 (BG04_2264) - 2107843..2108736 (-) 894 WP_013085559.1 mechanosensitive ion channel family protein -
  BG04_RS11390 (BG04_2265) - 2108804..2109817 (-) 1014 WP_013059919.1 membrane protein -
  BG04_RS11395 (BG04_2266) yyaC 2109904..2110524 (+) 621 WP_016765991.1 spore protease YyaC -
  BG04_RS11400 (BG04_2267) - 2110557..2111426 (-) 870 WP_013059921.1 ParB/RepB/Spo0J family partition protein -
  BG04_RS11405 (BG04_2268) - 2111398..2112180 (-) 783 WP_013085563.1 ParA family protein -
  BG04_RS11410 (BG04_2269) noc 2112297..2113172 (-) 876 WP_013059923.1 nucleoid occlusion protein -
  BG04_RS11415 (BG04_2270) rsmG 2113308..2114024 (-) 717 WP_013085565.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  BG04_RS11420 (BG04_2271) mnmG 2114055..2115944 (-) 1890 WP_013059925.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG -
  BG04_RS11425 (BG04_2272) mnmE 2115967..2117355 (-) 1389 WP_013085566.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  BG04_RS11430 (BG04_2273) jag 2117661..2118290 (-) 630 WP_013085567.1 RNA-binding cell elongation regulator Jag/EloR -
  BG04_RS11435 (BG04_2274) spoIIIJ 2118287..2119051 (-) 765 WP_034648179.1 YidC family membrane integrase SpoIIIJ -
  BG04_RS11440 (BG04_2275) rnpA 2119167..2119520 (-) 354 WP_013059929.1 ribonuclease P protein component -
  BG04_RS11445 (BG04_2276) rpmH 2119631..2119765 (-) 135 WP_013059930.1 50S ribosomal protein L34 -
  BG04_RS11450 (BG04_2277) dnaA 2120520..2121863 (+) 1344 WP_013081315.1 chromosomal replication initiator protein DnaA -
  BG04_RS11455 (BG04_2278) dnaN 2122050..2123186 (+) 1137 WP_013054821.1 DNA polymerase III subunit beta -
  BG04_RS11460 (BG04_2279) yaaA 2123287..2123502 (+) 216 WP_013054822.1 S4 domain-containing protein YaaA -
  BG04_RS11465 (BG04_2280) recF 2123517..2124635 (+) 1119 WP_013054823.1 DNA replication/repair protein RecF -
  BG04_RS11470 (BG04_2281) gyrB 2124876..2126792 (+) 1917 WP_013081316.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  BG04_RS11475 (BG04_2282) gyrA 2126919..2129429 (+) 2511 WP_016762686.1 DNA gyrase subunit A -
  BG04_RS11505 (BG04_2288) - 2134733..2135698 (-) 966 WP_034648173.1 YaaC family protein -
  BG04_RS11510 (BG04_2289) guaB 2135815..2137281 (+) 1467 WP_013081319.1 IMP dehydrogenase -
  BG04_RS11515 (BG04_2290) - 2137431..2138786 (+) 1356 WP_034648171.1 serine hydrolase -
  BG04_RS11520 (BG04_2291) pdxS 2138976..2139863 (+) 888 WP_013054829.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
  BG04_RS11525 (BG04_2292) pdxT 2139885..2140472 (+) 588 WP_016762689.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
  BG04_RS11530 (BG04_2293) serS 2140795..2142072 (+) 1278 WP_016762690.1 serine--tRNA ligase -
  BG04_RS11540 (BG04_2295) - 2142397..2143077 (-) 681 WP_013054832.1 deoxynucleoside kinase -
  BG04_RS11545 (BG04_2296) - 2143077..2143712 (-) 636 WP_013081322.1 deoxynucleoside kinase -
  BG04_RS11550 (BG04_2297) - 2143856..2145157 (-) 1302 WP_013081323.1 glycoside hydrolase family 18 protein -
  BG04_RS11555 (BG04_2298) - 2145214..2145756 (-) 543 WP_016762693.1 isochorismatase family cysteine hydrolase -
  BG04_RS11560 (BG04_2299) tadA 2145836..2146306 (+) 471 WP_080743114.1 tRNA adenosine(34) deaminase TadA -
  BG04_RS11565 (BG04_2300) dnaX 2146804..2148495 (+) 1692 WP_034648165.1 DNA polymerase III subunit gamma/tau -

Sequence


Protein


Download         Length: 166 a.a.        Molecular weight: 18132.76 Da        Isoelectric Point: 4.6529

>NTDB_id=132967 BG04_RS11365 WP_013059914.1 2105336..2105836(-) (ssbA) [Priestia megaterium NBRC 15308 = ATCC 14581]
MMNRVILVGRLTKDPELRYTPSGAAVATFTLAVNRTFTNQQGEREADFINCVVWRRQAENAANFLKKGSLAGVDGRLQSR
SYEGQDGRRVYVTEVVAESVQFLEPKSGGGDTQRSNFANTGGSGQGSPFGDNQNRNSGNQGYTRVDDDPFANNGETIDIS
DDDLPF

Nucleotide


Download         Length: 501 bp        

>NTDB_id=132967 BG04_RS11365 WP_013059914.1 2105336..2105836(-) (ssbA) [Priestia megaterium NBRC 15308 = ATCC 14581]
ATGATGAATCGCGTAATTCTCGTAGGACGCTTAACCAAAGATCCTGAATTACGTTATACCCCGAGTGGAGCAGCGGTTGC
AACATTCACTTTAGCGGTAAACCGCACATTTACAAATCAGCAAGGTGAACGTGAAGCTGACTTCATCAACTGTGTTGTAT
GGCGTCGTCAGGCTGAAAATGCAGCCAATTTCCTTAAAAAAGGAAGTTTAGCTGGTGTTGATGGTCGACTACAATCTCGC
AGTTACGAAGGACAAGACGGTCGTCGCGTATACGTAACGGAAGTTGTAGCGGAAAGTGTGCAATTTTTAGAGCCGAAAAG
TGGCGGTGGGGATACGCAACGCAGTAACTTTGCTAACACTGGAGGATCTGGACAAGGCTCTCCATTTGGAGATAATCAGA
ACCGTAACTCAGGTAATCAAGGTTATACTCGCGTTGATGATGATCCATTTGCAAATAATGGCGAGACAATTGACATTTCA
GATGACGATTTACCGTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0B6A5N1

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

72.571

100

0.765

  ssb Latilactobacillus sakei subsp. sakei 23K

58.721

100

0.608

  ssbB Bacillus subtilis subsp. subtilis str. 168

63.208

63.855

0.404

  ssb Vibrio cholerae strain A1552

33.889

100

0.367

  ssb Glaesserella parasuis strain SC1401

34.463

100

0.367


Multiple sequence alignment