Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OY18_RS12595 Genome accession   NZ_CP009823
Coordinates   2527475..2529208 (+) Length   577 a.a.
NCBI ID   WP_060871718.1    Uniprot ID   -
Organism   Xylella fastidiosa strain J1a12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2522475..2534208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OY18_RS12560 (OY18_11820) pilC 2522781..2523983 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  OY18_RS14765 - 2523913..2524092 (+) 180 WP_195780022.1 hypothetical protein -
  OY18_RS12570 (OY18_11825) - 2524423..2524869 (+) 447 WP_060871716.1 pilin -
  OY18_RS12575 (OY18_11830) - 2525130..2525333 (-) 204 WP_010894961.1 hypothetical protein -
  OY18_RS12580 (OY18_11835) - 2526167..2526634 (+) 468 WP_023907060.1 pilin -
  OY18_RS12595 (OY18_11845) pilB 2527475..2529208 (+) 1734 WP_060871718.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OY18_RS12600 (OY18_11850) pilR 2529302..2530714 (-) 1413 WP_060871719.1 sigma-54 dependent transcriptional regulator Regulator
  OY18_RS12605 (OY18_11855) - 2530900..2532510 (-) 1611 WP_060871720.1 ATP-binding protein -
  OY18_RS12610 (OY18_11860) sucC 2532759..2533922 (+) 1164 WP_046418841.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62992.06 Da        Isoelectric Point: 5.9154

>NTDB_id=132432 OY18_RS12595 WP_060871718.1 2527475..2529208(+) (pilB) [Xylella fastidiosa strain J1a12]
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSTAKLAAANALEFGMPLLDVSAFDHN
HNAMSLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALE
EEDNGIEGLDIGGGDDDGASGDTGIDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPVQLPT
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGITGLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=132432 OY18_RS12595 WP_060871718.1 2527475..2529208(+) (pilB) [Xylella fastidiosa strain J1a12]
ATGAACACACCTCATTCAGCCAATCTTGTCGGCATCACCGGCATTGCCCGTCGCCTAGTCCAGGATGGCAGCATCGAGGA
AGCCACCGCACGCACTGCTCAAAATCAAGCAATGGCCGCAAAAGTGCCTTTACCACAGTGGTTGATCGATAAAAAACTAG
TAAGTACCGCAAAGTTGGCCGCAGCCAATGCACTTGAGTTCGGGATGCCGCTGCTGGATGTCTCCGCATTCGACCACAAT
CACAATGCAATGAGCTTGGTCGACGAAGAATTACTGCAAAAGCATCGGGTGTTGCCATTATTTAAACGCGGTAATCGACT
GTTCGTCGGGATCAGCAATCCAACCCAAACACAGGCCCTGGACGACATCAAATTCCACACCAATCTGGTCGTGGAACCGA
TTCTGGTCGACGAAGACCAGATACGACGCACACTTGAACAATGGCAATCTAGGAGCGGCGCGCTCAGCTCCGCATTGGAG
GAAGAGGATAACGGCATTGAAGGGCTGGATATCGGCGGCGGAGACGACGACGGGGCCAGTGGCGATACAGGGATTGATGC
CAAGAGCGATGACACACCTGTCGTCAAATTCGTCAACAAAGTGCTGATTGATGCCATCCGACGCGGCGCATCAGACATCC
ACTTTGAACCATACGAAGAAGATTACCGCGTCCGTTTCCGGATTGATGGTCTGCTGAAGGCTGTGGCAAAAGCACCGAAC
AAGTTGAATCAGCGTATCGCCGCACGCTTGAAAGTCATGTCGCAACTGGATATCGCGGAAAAACGCGTCCCTCAAGACGG
ACGTATCAAGCTTAATCTGTCGAAAACCAAGCAAGTCGATTTCCGCGTCAGCACCTTGCCAACGCTGTTCGGCGAGAAAG
TGGTACTGCGTATCTTGGATGGCAGCGCGGCAAAGCTGGGTATCGACAAACTCGGTTACGAAGCTGACCAACAAACGCTT
TTTATGGAAGCAATCCACAAACCCTATGGAATGGTATTGGTCACTGGTCCGACCGGTTCGGGCAAGACAGTGTCACTGTA
CACTGCACTAGGCATCCTCAATGACGAAACACGCAATATCTCCACAGCCGAAGATCCTGTAGAAATCCGGCTGCCGGGCG
TCAATCAGGTACAACAAAACAACAAGCGCGGTATGACGTTTGCCGCAGCATTACGTTCGTTTCTGCGCCAAGACCCGGAT
GTCATCATGGTCGGCGAAATCCGGGATCTGGAAACCGCTGAAATCGCCATCAAAGCCGCACAGACCGGCCACATGGTGCT
CTCCACACTACATACCAACGATGCACCGCAAACCATCGCACGCCTCATGAACATGGGGATTGCACCATACAACATCACTT
CATCCGTGACCTTAGTGATTGCACAGCGATTGGCACGGAGATTGTGCAACAAGTGCAAGCGCCCGGTTCAATTGCCGACA
AACGCATTACTGGCCGAAGGATTCACTCAAGAACAAATTGATGCGGGGCTGGAATTATACGAGCCTGTCGGTTGCGACGA
ATGCACCGAAGGCTACAAAGGCCGCACAGGTATTTATCAAGTGATGCCAATTACCGAAGGGATTTCCTCGATCATTTTAG
CCGGCGGTAATGCCTTACAAATCGCCGAAGAGGCGCAGCGCATCGGCATCAACGACCTACGCCAATCGGCGCTACTCAAA
GTCGCTCAGGGCATCACCGGGCTGGCTGAAATTAATCGAGTCACCAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.143

98.267

0.562
  pilB Acinetobacter baumannii D1279779

56.437

98.267

0.555
  pilB Legionella pneumophila strain ERS1305867

51.761

98.44

0.51
  pilF Neisseria gonorrhoeae MS11

50.439

98.614

0.497
  pilB Vibrio cholerae strain A1552

49.724

94.107

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

47.08

97.92

0.461
  pilB Vibrio campbellii strain DS40M4

46.737

98.267

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.993

92.894

0.362
  pilF Thermus thermophilus HB27

40.077

89.948

0.36

Multiple sequence alignment