Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AMD14_RS15250 Genome accession   NZ_CP008998
Coordinates   3406291..3407421 (-) Length   376 a.a.
NCBI ID   WP_003489921.1    Uniprot ID   A0AAI7ZGP8
Organism   Xanthomonas citri pv. citri strain MN12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3401291..3412421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMD14_RS15220 (J164_03077) ruvX 3401798..3402265 (-) 468 WP_011051923.1 Holliday junction resolvase RuvX -
  AMD14_RS15225 (J164_03078) - 3402258..3402824 (-) 567 WP_011051924.1 YqgE/AlgH family protein -
  AMD14_RS15235 (J164_03079) - 3403113..3403685 (+) 573 WP_011051925.1 DNA-3-methyladenine glycosylase I -
  AMD14_RS15240 (J164_03080) - 3403693..3404619 (+) 927 WP_170913509.1 DUF72 domain-containing protein -
  AMD14_RS24890 - 3404910..3405161 (+) 252 WP_162010796.1 hypothetical protein -
  AMD14_RS15245 (J164_03082) - 3405197..3406201 (+) 1005 WP_005926112.1 pectate lyase -
  AMD14_RS15250 (J164_03083) pilU 3406291..3407421 (-) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMD14_RS15255 (J164_03084) pilT 3407544..3408491 (-) 948 WP_336884681.1 type IV pilus twitching motility protein PilT Machinery gene
  AMD14_RS15260 (J164_03085) - 3408994..3409686 (+) 693 WP_003489910.1 YggS family pyridoxal phosphate-dependent enzyme -
  AMD14_RS15265 (J164_03086) proC 3409730..3410584 (+) 855 WP_011051928.1 pyrroline-5-carboxylate reductase -
  AMD14_RS15270 (J164_03087) - 3411143..3411568 (+) 426 WP_003489904.1 HU family DNA-binding protein -
  AMD14_RS15275 (J164_03088) - 3411675..3412313 (+) 639 WP_015472435.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42044.26 Da        Isoelectric Point: 6.8286

>NTDB_id=126004 AMD14_RS15250 WP_003489921.1 3406291..3407421(-) (pilU) [Xanthomonas citri pv. citri strain MN12]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=126004 AMD14_RS15250 WP_003489921.1 3406291..3407421(-) (pilU) [Xanthomonas citri pv. citri strain MN12]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATTACCCAGACCCCGCTGACCGCGCAGCAGAGTCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTTCGTGTCAGCTGCTTCTACCAGCGTAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGGACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCATTCGCCGAAACCGGCCATCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGATCGCCGCAACCAGCTGTTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGACGGGCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATACGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAAATCAGTTACGAAGACGCGTTGCGCTACGCCGACTCGCAGAACGA
GGTCCGCCTGCGCATCAAGCTCTCGCAGGGCGGCGATGCCAAGACCTTGTCGCAGGGCCTGGACGGCGTGGAAATTTCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.457

94.947

0.394
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364

Multiple sequence alignment