Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AMD14_RS15255 Genome accession   NZ_CP008998
Coordinates   3407544..3408491 (-) Length   315 a.a.
NCBI ID   WP_336884681.1    Uniprot ID   -
Organism   Xanthomonas citri pv. citri strain MN12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3402544..3413491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMD14_RS15235 (J164_03079) - 3403113..3403685 (+) 573 WP_011051925.1 DNA-3-methyladenine glycosylase I -
  AMD14_RS15240 (J164_03080) - 3403693..3404619 (+) 927 WP_170913509.1 DUF72 domain-containing protein -
  AMD14_RS24890 - 3404910..3405161 (+) 252 WP_162010796.1 hypothetical protein -
  AMD14_RS15245 (J164_03082) - 3405197..3406201 (+) 1005 WP_005926112.1 pectate lyase -
  AMD14_RS15250 (J164_03083) pilU 3406291..3407421 (-) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMD14_RS15255 (J164_03084) pilT 3407544..3408491 (-) 948 WP_336884681.1 type IV pilus twitching motility protein PilT Machinery gene
  AMD14_RS15260 (J164_03085) - 3408994..3409686 (+) 693 WP_003489910.1 YggS family pyridoxal phosphate-dependent enzyme -
  AMD14_RS15265 (J164_03086) proC 3409730..3410584 (+) 855 WP_011051928.1 pyrroline-5-carboxylate reductase -
  AMD14_RS15270 (J164_03087) - 3411143..3411568 (+) 426 WP_003489904.1 HU family DNA-binding protein -
  AMD14_RS15275 (J164_03088) - 3411675..3412313 (+) 639 WP_015472435.1 M23 family metallopeptidase -
  AMD14_RS15280 (J164_03089) - 3412371..3412712 (+) 342 WP_011051930.1 DUF3861 family protein -
  AMD14_RS25575 (J164_03090) - 3412769..3413311 (+) 543 WP_005913301.1 hypothetical protein -

Sequence

Protein


Download         Length: 315 a.a.        Molecular weight: 35099.18 Da        Isoelectric Point: 9.4678

>NTDB_id=126005 AMD14_RS15255 WP_336884681.1 3407544..3408491(-) (pilT) [Xanthomonas citri pv. citri strain MN12]
MAMSAASIFRRWTTNRCTRWCTTSCRTSSGAITRNSSKSTFSFEIPSLARFRVNAFNQNRGAGAVFRTIPSEVLTLEDLG
CPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVEDPIEFVHTSQKCLINQREVHRDTHGFNEALR
SALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRIIDVFPAGEKPMVRSMLSESLRAVISQALLK
KVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHLQDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 948 bp        

>NTDB_id=126005 AMD14_RS15255 WP_336884681.1 3407544..3408491(-) (pilT) [Xanthomonas citri pv. citri strain MN12]
ATGGCGATGTCCGCCGCATCAATATTCCGGCGTTGGACCACAAACAGGTGCACGCGCTGGTGTACGACATCATGTCGGAC
AAGCAGCGGCGCGATTACGAGGAATTCCTCGAAGTCGACCTTTTCGTTCGAAATTCCGTCGCTGGCGCGTTTTCGCGTCA
ATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACGCTGGAAGACCTTGGC
TGCCCGCCGATCTTCCGTCAGCTGATCGATCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAGTC
GACCACGCTGGCCGGCATGATCGACTACATCAACAAGAATGAGTACGGGCACATCCTCACCGTCGAGGACCCGATCGAAT
TCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGCACGGCTTCAACGAAGCGCTGCGC
TCGGCACTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATTCGCCTGGCGCTCACCGC
TGCGGAAACCGGTCACTTGGTGTTCGGCACCCTGCACACCAGCTCGGCCGCCAAGACCATCGACCGCATCATCGACGTGT
TCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCTGTGATTTCGCAGGCGCTGCTGAAG
AAGGTCGGCGGCGGCCGTACCGCAGCATGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCCGCGAGGACAA
GGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTGCAGGACCTGG
TCAAGCGCAGCCTGATCACGCGCAACCAGGCTCGCGAGTACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(50-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.978

87.937

0.686
  pilT Acinetobacter baumannii D1279779

77.978

87.937

0.686
  pilT Acinetobacter baumannii strain A118

77.978

87.937

0.686
  pilT Pseudomonas stutzeri DSM 10701

77.617

87.937

0.683
  pilT Acinetobacter baylyi ADP1

77.617

87.937

0.683
  pilT Pseudomonas aeruginosa PAK

77.206

86.349

0.667
  pilT Legionella pneumophila strain ERS1305867

75.09

87.937

0.66
  pilT Legionella pneumophila strain Lp02

75.09

87.937

0.66
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.587

82.222

0.597
  pilT Vibrio cholerae strain A1552

72.587

82.222

0.597
  pilT Neisseria meningitidis 8013

68.266

86.032

0.587
  pilT Neisseria gonorrhoeae MS11

67.897

86.032

0.584
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.818

87.302

0.47
  pilU Pseudomonas stutzeri DSM 10701

42.701

86.984

0.371

Multiple sequence alignment