Detailed information NTG0125434

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HY57_RS07680
• Genome accession: NZ_CP008884
• Coordinates: 1865058..1866224 (+)
• Length: 388 a.a.
• NCBI ID: WP_019464694.1
• Uniprot ID: A0A075K040
• Organism: Dyella japonica A8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1860058..1871224

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HY57_RS07665 (HY57_07815)	-	1862177..1862752 (-)	576	WP_019464697.1	YggT family protein	-
HY57_RS07670 (HY57_07820)	proC	1862749..1863573 (-)	825	WP_019464696.1	pyrroline-5-carboxylate reductase	-
HY57_RS07675 (HY57_07825)	pilT	1863898..1864938 (+)	1041	WP_019464695.1	type IV pilus twitching motility protein PilT	Machinery gene
HY57_RS07680 (HY57_07830)	pilU	1865058..1866224 (+)	1167	WP_019464694.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HY57_RS07685 (HY57_07835)	-	1866302..1866799 (-)	498	WP_235186631.1	BON domain-containing protein	-
HY57_RS07690 (HY57_07840)	-	1867181..1867921 (+)	741	WP_039732306.1	hypothetical protein	-
HY57_RS07695 (HY57_07845)	-	1867986..1868729 (+)	744	WP_019464691.1	MHYT domain-containing protein	-
HY57_RS07700 (HY57_07850)	-	1868805..1870568 (-)	1764	WP_019464690.1	hypothetical protein	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 43396.71 Da
• Isoelectric Point: 6.9604

>NTDB_id=125434 HY57_RS07680 WP_019464694.1 1865058..1866224(+) (pilU) [Dyella japonica A8]
MSDFDFTSFLKLMVHKKASDLFITAGVAPSMKVQGRIVPITQSPLSAQQSRDMVLNVMTPSQREEFEKTHECQFAISAQG
VGRFRVSCFYQRNCVGMVLRRIESKIPTIEELSLPPVIKQLAMTKRGIIIFVGGTGTGKSTSLASMIGYRNANSTGHIIT
IEDPIEYVHKHEGCIITQRELGIDTDSWDNALKNTLRQAPDVIMIGEVRTRETMEHAINFSETGHLCLCTLHANNANQAM
DRILHFFPEDRRSQLFMDLSLNLKGVVAQQLIPTPDGKARRVAVEVLLGTPLVQDYIRQGEIHKLKEVMKESTNLGMKTF
DQSLVELYHAGEISYEDALRHADSSNEVRLRIKLAQGGDAHTLSQGLDGVELEETKDSQNFSNGLLRR

Nucleotide

• Length: 1167 bp

>NTDB_id=125434 HY57_RS07680 WP_019464694.1 1865058..1866224(+) (pilU) [Dyella japonica A8]
ATGAGCGACTTCGATTTCACCTCCTTCCTCAAGTTGATGGTGCACAAGAAGGCCTCCGACCTGTTCATCACGGCGGGTGT
GGCGCCTTCGATGAAGGTGCAGGGGCGCATCGTGCCGATCACGCAGAGCCCCCTGTCCGCGCAGCAGTCGCGCGACATGG
TGCTCAACGTGATGACGCCATCGCAGCGCGAAGAGTTCGAAAAGACCCACGAGTGCCAGTTCGCCATCTCGGCGCAGGGT
GTGGGCCGCTTCCGTGTGTCGTGCTTCTACCAGCGCAACTGCGTGGGCATGGTGCTTCGCCGCATCGAGTCGAAGATTCC
CACCATCGAAGAGCTCAGCCTGCCGCCGGTGATCAAGCAGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
GCACGGGCACCGGTAAGTCCACCTCGCTGGCGTCGATGATCGGCTACCGCAACGCCAATTCGACCGGCCACATCATCACC
ATCGAAGACCCGATCGAATACGTGCACAAGCACGAGGGCTGCATCATCACGCAGCGCGAGCTCGGCATCGATACCGACAG
CTGGGACAACGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTCATCATGATCGGCGAAGTGCGTACGCGCGAAACCA
TGGAGCACGCGATCAACTTCTCGGAAACGGGCCACCTGTGCCTGTGTACGCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGCATCCTGCACTTCTTCCCGGAAGACCGCCGTTCGCAGCTGTTCATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
GGCGCAGCAGCTCATCCCCACGCCGGATGGCAAGGCGCGTCGCGTGGCGGTGGAAGTGCTGCTGGGTACGCCGCTGGTGC
AGGACTACATCCGCCAAGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAATCCACCAACCTCGGCATGAAGACCTTC
GATCAGAGCCTGGTCGAGCTCTACCACGCGGGCGAGATCTCCTACGAGGATGCGCTTCGTCATGCGGACAGCTCCAATGA
AGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGATGCGCATACGCTTTCGCAGGGCCTGGACGGTGTGGAGCTGGAGG
AGACGAAGGATTCGCAGAACTTCAGCAACGGCCTGCTGCGCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (83-272)

3D structure

• AlphaFold DB: A0A075K040

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	66.31	96.392	0.639
pilU	Acinetobacter baylyi ADP1	62.64	91.753	0.575
pilU	Vibrio cholerae strain A1552	51.966	91.753	0.477
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.591	95.876	0.389