Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   HW00_RS28965 Genome accession   NZ_CP008867
Coordinates   1278311..1278847 (-) Length   178 a.a.
NCBI ID   WP_025921208.1    Uniprot ID   Q8KQ36
Organism   Pseudomonas aeruginosa strain T52373     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1277509..1289480 1278311..1278847 within 0


Gene organization within MGE regions


Location: 1277509..1289480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW00_RS29855 tfpZ 1277509..1278246 (-) 738 WP_023110409.1 TfpX/TfpZ family type IV pilin accessory protein -
  HW00_RS28965 (HW00_05945) pilE 1278311..1278847 (-) 537 WP_025921208.1 pilin Machinery gene
  HW00_RS05950 (HW00_05950) pilB 1279074..1280777 (+) 1704 WP_023100230.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HW00_RS05955 (HW00_05955) pilC 1280781..1281998 (+) 1218 WP_023103506.1 type II secretion system F family protein Machinery gene
  HW00_RS05960 (HW00_05960) pilD 1282001..1282873 (+) 873 WP_059317084.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HW00_RS05965 (HW00_05965) coaE 1282870..1283481 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  HW00_RS05970 (HW00_05970) yacG 1283478..1283678 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  HW00_RS05975 (HW00_05975) - 1283715..1283924 (-) 210 WP_003094660.1 hypothetical protein -
  HW00_RS05980 (HW00_05980) - 1284030..1284719 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  HW00_RS05985 (HW00_05985) - 1284716..1285186 (-) 471 WP_003094664.1 hypothetical protein -
  HW00_RS05990 (HW00_05990) - 1285183..1285608 (-) 426 WP_033951923.1 GNAT family N-acetyltransferase -
  HW00_RS05995 (HW00_05995) - 1285741..1286370 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  HW00_RS06000 (HW00_06000) - 1286367..1286816 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  HW00_RS28970 - 1286842..1287011 (+) 170 Protein_1203 DUF3094 family protein -
  HW00_RS06010 (HW00_06010) - 1287075..1288382 (+) 1308 WP_003112837.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 178 a.a.        Molecular weight: 18322.83 Da        Isoelectric Point: 4.9984

>NTDB_id=125152 HW00_RS28965 WP_025921208.1 1278311..1278847(-) (pilE) [Pseudomonas aeruginosa strain T52373]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRTRVSEGLVLAEPAKLMISTDGSASTADLTRATTTWNQQSNNLGA
SSKYVTSVLMDAGNTGVITITYVADQVGLPTAGNTLILSPYINDGNTRTALATAVAAGTRGTIDWACTSASNATATAQGF
TGMAAGSVPQEFAPAQCR

Nucleotide


Download         Length: 537 bp        

>NTDB_id=125152 HW00_RS28965 WP_025921208.1 1278311..1278847(-) (pilE) [Pseudomonas aeruginosa strain T52373]
ATGAAAGCTCAAAAAGGCTTCACTTTGATCGAGCTGATGATCGTTGTAGCGATCATCGGTATTCTGGCTGCGATTGCTCT
GCCGGCCTATCAGGACTACACCGTTCGTACCCGTGTCAGTGAAGGTCTGGTTCTGGCTGAGCCCGCCAAACTGATGATCT
CCACGGATGGAAGTGCTTCTACTGCTGATCTGACTCGCGCAACCACTACTTGGAACCAGCAATCCAATAATCTGGGTGCA
TCCAGTAAGTACGTAACATCTGTTTTGATGGATGCTGGTAACACCGGTGTTATCACTATTACGTATGTCGCTGACCAAGT
GGGCTTGCCGACTGCCGGTAACACTCTGATTCTGTCTCCCTATATCAATGATGGTAACACTCGCACTGCTCTGGCGACTG
CAGTGGCTGCTGGCACTCGTGGCACCATTGATTGGGCTTGCACTTCGGCATCGAACGCGACTGCCACTGCTCAGGGTTTC
ACCGGCATGGCAGCTGGTAGTGTGCCGCAAGAGTTTGCTCCTGCTCAGTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 3JYZ
  PDB 3JZZ

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

43.719

100

0.489
  pilA Ralstonia pseudosolanacearum GMI1000

40.556

100

0.41
  pilA2 Legionella pneumophila strain ERS1305867

40.341

98.876

0.399
  pilA2 Legionella pneumophila str. Paris

39.773

98.876

0.393

Multiple sequence alignment