Detailed information NTG0125120

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: HW00_RS05950
• Genome accession: NZ_CP008867
• Coordinates: 1279074..1280777 (+)
• Length: 567 a.a.
• NCBI ID: WP_023100230.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain T52373
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1277509..1289480	1279074..1280777	within	0

Gene organization within MGE regions

Location: 1277509..1289480

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HW00_RS29855	tfpZ	1277509..1278246 (-)	738	WP_023110409.1	TfpX/TfpZ family type IV pilin accessory protein	-
HW00_RS28965 (HW00_05945)	pilE	1278311..1278847 (-)	537	WP_025921208.1	pilin	Machinery gene
HW00_RS05950 (HW00_05950)	pilB	1279074..1280777 (+)	1704	WP_023100230.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HW00_RS05955 (HW00_05955)	pilC	1280781..1281998 (+)	1218	WP_023103506.1	type II secretion system F family protein	Machinery gene
HW00_RS05960 (HW00_05960)	pilD	1282001..1282873 (+)	873	WP_059317084.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
HW00_RS05965 (HW00_05965)	coaE	1282870..1283481 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
HW00_RS05970 (HW00_05970)	yacG	1283478..1283678 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
HW00_RS05975 (HW00_05975)	-	1283715..1283924 (-)	210	WP_003094660.1	hypothetical protein	-
HW00_RS05980 (HW00_05980)	-	1284030..1284719 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
HW00_RS05985 (HW00_05985)	-	1284716..1285186 (-)	471	WP_003094664.1	hypothetical protein	-
HW00_RS05990 (HW00_05990)	-	1285183..1285608 (-)	426	WP_033951923.1	GNAT family N-acetyltransferase	-
HW00_RS05995 (HW00_05995)	-	1285741..1286370 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
HW00_RS06000 (HW00_06000)	-	1286367..1286816 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
HW00_RS28970	-	1286842..1287011 (+)	170	Protein_1203	DUF3094 family protein	-
HW00_RS06010 (HW00_06010)	-	1287075..1288382 (+)	1308	WP_003112837.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 567 a.a.
• Molecular weight: 62580.51 Da
• Isoelectric Point: 5.5586

>NTDB_id=125120 HW00_RS05950 WP_023100230.1 1279074..1280777(+) (pilB) [Pseudomonas aeruginosa strain T52373]
MNDTIQLSGLARQLVLHELLDEKAAQQAQLQAQRNKLSLVTYLVQSKLVKGQALTELAADQFGIAYCDLNSLDRDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRASGLLKAMQGITSLEE
VNRVTKD

Nucleotide

• Length: 1704 bp

>NTDB_id=125120 HW00_RS05950 WP_023100230.1 1279074..1280777(+) (pilB) [Pseudomonas aeruginosa strain T52373]
ATGAACGATACCATCCAGCTCAGCGGCCTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAAGCAGCTCAACA
GGCCCAGTTGCAAGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGACAGGCCT
TGACCGAACTGGCGGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGATCGGGACAGCCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGGGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGCCTGGATGACGTGGATCTTGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTATTGGATGCGATCAGAGGCGGCTCCTCTGATCTGCATTTCGAGCCCTATGAAAAGATTTACC
GTGTACGTTTCCGTACCGATGGCATGCTCCATGAGGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTAATGGCAGGCCTGGACATTTCCGAACGGCGTAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTTAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGTATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTAGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGACCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAAC
AGGCCCGCAAAGAAGGCTTCAACGATCTACGCGCTTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (191-458)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	58.07	100	0.584
pilB	Acinetobacter baylyi ADP1	57.447	99.471	0.571
pilB	Legionella pneumophila strain ERS1305867	55.282	100	0.554
pilF	Neisseria gonorrhoeae MS11	50.444	99.295	0.501
pilB	Vibrio parahaemolyticus RIMD 2210633	51.763	95.062	0.492
pilB	Vibrio cholerae strain A1552	50.545	97.002	0.49
pilB	Vibrio campbellii strain DS40M4	50.832	95.414	0.485
pilF	Thermus thermophilus HB27	38.584	99.647	0.384
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.189	93.474	0.376