Detailed information NTG0124781

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HW10_RS02125
• Genome accession: NZ_CP008856
• Coordinates: 441575..442723 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain F23197
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 436575..447723

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HW10_RS02100 (HW10_30155)	-	437726..438022 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HW10_RS02105 (HW10_11295)	-	438019..438612 (-)	594	WP_003084544.1	YggT family protein	-
HW10_RS02110 (HW10_11290)	proC	438623..439444 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HW10_RS02115 (HW10_11285)	-	439456..440148 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HW10_RS02120 (HW10_11280)	pilT	440363..441397 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HW10_RS02125 (HW10_11275)	pilU	441575..442723 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HW10_RS02130 (HW10_11270)	czcD	442731..443630 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HW10_RS02135 (HW10_11265)	-	443784..444188 (+)	405	WP_003084560.1	NINE protein	-
HW10_RS02140 (HW10_11260)	-	444431..445804 (+)	1374	WP_003459266.1	pyridoxal-phosphate dependent enzyme	-
HW10_RS02145 (HW10_11255)	-	445801..446985 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=124781 HW10_RS02125 WP_003100959.1 441575..442723(+) (pilU) [Pseudomonas aeruginosa strain F23197]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=124781 HW10_RS02125 WP_003100959.1 441575..442723(+) (pilU) [Pseudomonas aeruginosa strain F23197]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGTATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382