Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EP13_RS14320 Genome accession   NZ_CP008849
Coordinates   3260683..3261795 (-) Length   370 a.a.
NCBI ID   WP_044057881.1    Uniprot ID   A0A7T0WIV7
Organism   Alteromonas australica strain H 17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3255683..3266795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EP13_RS14285 (EP13_14610) - 3255812..3256654 (-) 843 WP_044057875.1 mechanosensitive ion channel family protein -
  EP13_RS14290 (EP13_14615) - 3257013..3257207 (+) 195 WP_044057876.1 hypothetical protein -
  EP13_RS14295 (EP13_14620) - 3257357..3258085 (+) 729 WP_044057877.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EP13_RS14300 (EP13_14630) gshB 3258292..3259245 (+) 954 WP_044057878.1 glutathione synthase -
  EP13_RS14305 (EP13_14635) - 3259273..3259839 (+) 567 WP_081869512.1 YqgE/AlgH family protein -
  EP13_RS14310 (EP13_14640) ruvX 3259832..3260260 (+) 429 WP_044057879.1 Holliday junction resolvase RuvX -
  EP13_RS14315 (EP13_14645) - 3260289..3260618 (-) 330 WP_044057880.1 DUF2007 domain-containing protein -
  EP13_RS14320 (EP13_14650) pilU 3260683..3261795 (-) 1113 WP_044057881.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EP13_RS14325 (EP13_14655) pilT 3261805..3262848 (-) 1044 WP_044057882.1 type IV pilus twitching motility protein PilT Machinery gene
  EP13_RS14330 (EP13_14660) - 3262876..3263574 (+) 699 WP_044057883.1 YggS family pyridoxal phosphate-dependent enzyme -
  EP13_RS14335 (EP13_14665) proC 3263660..3264481 (+) 822 WP_044057884.1 pyrroline-5-carboxylate reductase -
  EP13_RS14340 (EP13_14670) - 3264503..3265042 (+) 540 WP_044057885.1 YggT family protein -
  EP13_RS14345 (EP13_14675) - 3265079..3265519 (+) 441 WP_044057886.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40637.46 Da        Isoelectric Point: 6.1426

>NTDB_id=124755 EP13_RS14320 WP_044057881.1 3260683..3261795(-) (pilU) [Alteromonas australica strain H 17]
MLDTYLQKMVSAQASDLFITAGFPVSAKINGKLTPLSEQVTTEHSALSLVEDAMNDSQKAAFHSTKECNFAIVREGIGRF
RCSAFWQRDQAGMVIRRIVTDIPQADDLGLPPVLKDIIMAKRGLVLFVGGTGTGKSTSLAALIGHRNQHSHGHILTIEDP
IEFVHEHKNCVVTQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTTDGKGRVAAIEILLNSPLVTDLIQRNEIGSLKEAMKKGKEQGMQSFDMAL
YSLYREGKIDVEQALHHADSPNDLRLMIKLDANTGASLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=124755 EP13_RS14320 WP_044057881.1 3260683..3261795(-) (pilU) [Alteromonas australica strain H 17]
ATGTTAGACACATATCTACAAAAAATGGTTAGCGCACAGGCTTCAGACTTATTTATTACTGCTGGGTTTCCAGTTAGCGC
AAAAATTAACGGTAAGTTAACCCCATTGAGTGAACAGGTCACTACAGAACATTCCGCGCTTTCTCTGGTTGAAGATGCTA
TGAATGACAGCCAGAAAGCGGCGTTCCATTCCACTAAAGAATGCAACTTTGCCATTGTACGAGAGGGCATAGGCCGATTT
CGTTGTTCTGCGTTCTGGCAGCGGGATCAAGCGGGAATGGTCATTCGTCGTATTGTCACTGACATCCCGCAAGCAGATGA
TTTGGGGCTGCCGCCAGTGTTAAAAGACATCATTATGGCTAAACGTGGACTGGTTTTATTTGTGGGCGGGACAGGCACAG
GTAAATCTACCTCTCTGGCGGCACTGATTGGTCATCGAAATCAGCACTCTCACGGGCATATTCTCACTATTGAAGACCCC
ATTGAATTTGTTCACGAACACAAAAATTGCGTGGTCACACAACGGGAAGTGGGCATAGATACACAGTCCTTCGACGATGC
GCTAAAAAGCTCATTGCGCCAAGCACCCGATGTCATTCTTATTGGTGAGATTCGGTCTATGGAAACCATGGAATACGCAA
TGTCCTTTGCTGATACCGGCCATTTGTGCGTAGCGACGCTACATGCAAACAATGCCAATCAAGCCATAGAACGCATTATG
CACCTTGCGCCTAAAGATCAGCACGATAAACTGAGGTTTGATTTAAGTTTGAATATCCGTGCAATTGTGGCGCAGCAACT
TGTCCCTACTACCGACGGGAAAGGACGAGTGGCAGCGATTGAAATTTTACTGAATTCCCCGCTAGTGACAGATCTTATTC
AACGAAATGAAATCGGTAGTTTGAAAGAGGCAATGAAAAAAGGGAAAGAGCAGGGAATGCAAAGCTTTGATATGGCGCTC
TATTCACTTTACCGTGAAGGCAAAATTGATGTGGAGCAAGCGTTGCATCATGCGGATTCACCGAACGATCTGCGGCTAAT
GATTAAACTTGATGCCAATACAGGGGCTTCGTTAGGCACCCTATCTAACGTCTCAATCGACATGGATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T0WIV7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.03

100

0.592
  pilU Vibrio cholerae strain A1552

56.793

99.459

0.565
  pilU Acinetobacter baylyi ADP1

57.064

97.568

0.557
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.547

94.324

0.392

Multiple sequence alignment