Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EP13_RS14325 Genome accession   NZ_CP008849
Coordinates   3261805..3262848 (-) Length   347 a.a.
NCBI ID   WP_044057882.1    Uniprot ID   A0A075P268
Organism   Alteromonas australica strain H 17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3256805..3267848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EP13_RS14290 (EP13_14615) - 3257013..3257207 (+) 195 WP_044057876.1 hypothetical protein -
  EP13_RS14295 (EP13_14620) - 3257357..3258085 (+) 729 WP_044057877.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EP13_RS14300 (EP13_14630) gshB 3258292..3259245 (+) 954 WP_044057878.1 glutathione synthase -
  EP13_RS14305 (EP13_14635) - 3259273..3259839 (+) 567 WP_081869512.1 YqgE/AlgH family protein -
  EP13_RS14310 (EP13_14640) ruvX 3259832..3260260 (+) 429 WP_044057879.1 Holliday junction resolvase RuvX -
  EP13_RS14315 (EP13_14645) - 3260289..3260618 (-) 330 WP_044057880.1 DUF2007 domain-containing protein -
  EP13_RS14320 (EP13_14650) pilU 3260683..3261795 (-) 1113 WP_044057881.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EP13_RS14325 (EP13_14655) pilT 3261805..3262848 (-) 1044 WP_044057882.1 type IV pilus twitching motility protein PilT Machinery gene
  EP13_RS14330 (EP13_14660) - 3262876..3263574 (+) 699 WP_044057883.1 YggS family pyridoxal phosphate-dependent enzyme -
  EP13_RS14335 (EP13_14665) proC 3263660..3264481 (+) 822 WP_044057884.1 pyrroline-5-carboxylate reductase -
  EP13_RS14340 (EP13_14670) - 3264503..3265042 (+) 540 WP_044057885.1 YggT family protein -
  EP13_RS14345 (EP13_14675) - 3265079..3265519 (+) 441 WP_044057886.1 DUF4426 domain-containing protein -
  EP13_RS14350 (EP13_14680) - 3265652..3267673 (+) 2022 WP_231497873.1 NADPH-dependent 2,4-dienoyl-CoA reductase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38407.18 Da        Isoelectric Point: 6.6411

>NTDB_id=124756 EP13_RS14325 WP_044057882.1 3261805..3262848(-) (pilT) [Alteromonas australica strain H 17]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEMRKLNVPALDHKQVHGLVYEIMNDRQRKEYEENLETDFSFEVQGLSR
FRVNAFVQNRGAAAVLRTIPSKVLTLEELGAPDIFKSIINQPTGIVLVTGATGSGKSTTLAAMIDHINTQKREHILTIED
PIEFVHENKLSLINQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKSMVRSMLSESLRAVISQTLLKKVGGGRVAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQCL
QRLVAMGVITQQDAAAKLMDKSALANF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=124756 EP13_RS14325 WP_044057882.1 3261805..3262848(-) (pilT) [Alteromonas australica strain H 17]
GTGGATATTACCGAACTTTTGGCTTTCAGTGTTAAGAATAACGCCTCAGATTTACACTTGTCAGCAGGTTTACCCCCTAT
TATTCGGGTAGATGGCGAAATGAGAAAGCTTAATGTTCCCGCCCTCGACCATAAGCAAGTTCATGGGCTTGTGTACGAGA
TCATGAATGACAGGCAAAGAAAAGAATACGAAGAAAACCTTGAAACAGACTTTTCTTTTGAGGTGCAAGGATTATCACGA
TTTCGAGTGAATGCGTTTGTACAAAATAGAGGTGCGGCCGCGGTGTTGCGCACTATTCCAAGCAAAGTGTTGACCTTGGA
AGAGCTAGGTGCCCCTGATATTTTCAAATCTATTATTAATCAACCTACAGGCATTGTGCTTGTAACTGGCGCCACAGGCT
CGGGTAAAAGTACCACGCTGGCTGCCATGATTGATCATATAAATACGCAAAAAAGAGAGCATATCTTAACCATTGAAGAC
CCCATTGAGTTTGTGCATGAAAACAAGTTAAGCCTGATAAATCAGCGAGAAGTTCACAGAGACACCCACTCATTTAACAA
TGCATTACGCTCGGCGTTACGTGAAGATCCTGATGTAATATTGGTAGGTGAATTACGTGATTTAGAAACCATACGGTTAG
CAATATCGGCGGCTGAAACCGGCCACCTTGTATTTGGAACCTTACATACAAACTCAGCCCCTAAGACCATCGACCGTATC
ATTGATGTATTTCCTGCAGAAGAAAAGTCTATGGTACGTTCAATGTTATCGGAGTCCTTGCGTGCGGTGATTTCGCAAAC
TCTGCTTAAAAAAGTGGGCGGGGGCAGGGTTGCTGCTCATGAAATAATGGTGGGGATCCCCGCGATTAGAAACCTTATTC
GGGAAGATAAGGTGCCACAAATGTACTCAGTCATTCAAACAGGTCAAGCCCATGGTATGCAAACCATGGATCAATGTTTA
CAGCGCTTGGTGGCAATGGGTGTGATTACTCAGCAGGATGCTGCAGCCAAGTTAATGGACAAATCTGCCTTGGCGAATTT
TTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A075P268

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

77.876

97.695

0.761
  pilT Acinetobacter baumannii D1279779

77.876

97.695

0.761
  pilT Acinetobacter nosocomialis M2

77.581

97.695

0.758
  pilT Acinetobacter baylyi ADP1

76.19

96.83

0.738
  pilT Pseudomonas stutzeri DSM 10701

75.668

97.118

0.735
  pilT Pseudomonas aeruginosa PAK

75.074

97.118

0.729
  pilT Legionella pneumophila strain Lp02

71.93

98.559

0.709
  pilT Legionella pneumophila strain ERS1305867

71.93

98.559

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.189

97.406

0.703
  pilT Vibrio cholerae strain A1552

72.189

97.406

0.703
  pilT Neisseria meningitidis 8013

65.607

99.712

0.654
  pilT Neisseria gonorrhoeae MS11

65.318

99.712

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.586

100

0.527
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403
  pilU Acinetobacter baylyi ADP1

40.774

96.83

0.395

Multiple sequence alignment