Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VAA049_RS02420 Genome accession   NZ_CP010811
Coordinates   495292..495756 (-) Length   154 a.a.
NCBI ID   WP_000649341.1    Uniprot ID   -
Organism   Vibrio cholerae strain 1154-74     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 490292..500756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VAA049_RS02400 (VAA049_468) coaE 490788..491396 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  VAA049_RS02405 (VAA049_469) pilD 491393..492268 (-) 876 WP_046121365.1 A24 family peptidase Machinery gene
  VAA049_RS02410 (VAA049_470) pilC 492326..493552 (-) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  VAA049_RS02415 (VAA049_471) pilB 493595..495283 (-) 1689 WP_000957203.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VAA049_RS02420 (VAA049_472) pilA 495292..495756 (-) 465 WP_000649341.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VAA049_RS02425 (VAA049_473) nadC 496001..496891 (-) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VAA049_RS02430 (VAA049_474) ampD 497189..497734 (+) 546 WP_000567318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VAA049_RS02435 (VAA049_475) fldB 497885..498403 (-) 519 WP_000690112.1 flavodoxin FldB -
  VAA049_RS02440 (VAA049_476) xerD 498541..499449 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  VAA049_RS02445 (VAA049_477) dsbC 499466..500218 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16390.69 Da        Isoelectric Point: 7.7681

>NTDB_id=124390 VAA049_RS02420 WP_000649341.1 495292..495756(-) (pilA) [Vibrio cholerae strain 1154-74]
MKAYKNKQQQGFTLIELMIVVAIIGVLSAIAVPAYKDYVKKSEGASALATMKSLITASELWYQEKGSFKQTDSAAILTYL
GVKAGSNPMGEIAVSADNKLQFKFGNSSAVAQNTTITYDRKDTGWECNTSIADIATDSCPQAAATPTPPPTTTP

Nucleotide


Download         Length: 465 bp        

>NTDB_id=124390 VAA049_RS02420 WP_000649341.1 495292..495756(-) (pilA) [Vibrio cholerae strain 1154-74]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCGATTATTGGTGTGTT
ATCGGCTATTGCAGTGCCTGCTTATAAAGATTATGTAAAAAAATCTGAAGGCGCATCAGCATTAGCCACTATGAAATCAT
TAATCACAGCGTCAGAGCTTTGGTATCAAGAAAAAGGTTCATTTAAACAAACTGATTCTGCAGCTATTTTGACGTATCTT
GGAGTAAAAGCTGGATCAAATCCAATGGGTGAAATTGCTGTTTCTGCAGACAACAAACTACAATTTAAATTCGGTAACAG
TTCAGCTGTCGCACAAAATACAACCATTACCTATGACAGAAAAGATACTGGATGGGAATGTAATACTAGTATTGCTGATA
TCGCGACAGATAGTTGTCCTCAAGCAGCAGCCACACCAACACCACCACCAACAACAACACCATAG

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

48.252

92.857

0.448
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.791

99.351

0.435
  pilA Vibrio cholerae strain A1552

43.791

99.351

0.435
  pilA Vibrio cholerae C6706

43.791

99.351

0.435
  pilA Pseudomonas aeruginosa PAK

42.336

88.961

0.377