Detailed information NTG0123987

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSA3335_RS15765
• Genome accession: NZ_CP008742
• Coordinates: 3262391..3263560 (+)
• Length: 389 a.a.
• NCBI ID: WP_002553335.1
• Uniprot ID: A0A554H2G7
• Organism: Pseudomonas savastanoi pv. savastanoi NCPPB 3335
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3257391..3268560

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSA3335_RS15750 (PSA3335_14920)	-	3257829..3260156 (+)	2328	WP_002553332.1	ATP-dependent DNA helicase	-
PSA3335_RS15755 (PSA3335_14925)	pgm	3260271..3261917 (+)	1647	WP_002553333.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
PSA3335_RS15760 (PSA3335_14930)	-	3261992..3262267 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
PSA3335_RS15765 (PSA3335_14935)	pilU	3262391..3263560 (+)	1170	WP_002553335.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSA3335_RS15770 (PSA3335_14940)	-	3263611..3265196 (-)	1586	Protein_2959	ABC transporter ATP-binding protein	-
PSA3335_RS15775 (PSA3335_14945)	-	3265198..3266220 (-)	1023	WP_005732025.1	ABC transporter permease	-
PSA3335_RS15780 (PSA3335_14950)	-	3266220..3267284 (-)	1065	WP_002553338.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42548.76 Da
• Isoelectric Point: 6.0641

>NTDB_id=123987 PSA3335_RS15765 WP_002553335.1 3262391..3263560(+) (pilU) [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTAPPPAPIGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=123987 PSA3335_RS15765 WP_002553335.1 3262391..3263560(+) (pilU) [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGGGCAGTGAAACCCTCAAACCGGGCGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGATATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCTGTACTGCTCGATGTGATCATGGAAAAGCACGGCCTGGTGTTGTTCGTCGGTGCCACCGGCT
CGGGCAAATCGACCTCGTTGGCAGCGCTGATCGATTACCGCAACCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTCATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAGGTGGGGGTCGATACCCGCAGTTTCCGCGC
GGCGCTGAAGAACACGTTGCGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGACCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTTAGAACCCCGGACGGCAAACGTCGCGCAGCGGTGGAGGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAAGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTCAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGGCGCCGCCCCCGGCCCCGATCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGCGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

3D structure

• AlphaFold DB: A0A554H2G7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.011	93.059	0.54
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.948	98.458	0.393
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362