Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NMA510612_RS11050 Genome accession   NZ_CP007524
Coordinates   2108950..2110626 (-) Length   558 a.a.
NCBI ID   WP_002245855.1    Uniprot ID   -
Organism   Neisseria meningitidis strain 510612     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2103950..2115626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMA510612_RS11030 (NMA510612_2443) pilG 2105863..2107095 (+) 1233 WP_002216405.1 type 4 pilus assembly protein PilG Machinery gene
  NMA510612_RS11035 (NMA510612_2444) pilD 2107169..2108029 (+) 861 WP_002220276.1 A24 family peptidase Machinery gene
  NMA510612_RS11040 (NMA510612_2445) coaE 2108031..2108663 (+) 633 WP_002245856.1 dephospho-CoA kinase -
  NMA510612_RS11045 (NMA510612_2446) - 2108656..2108865 (+) 210 WP_002212333.1 DNA gyrase inhibitor YacG -
  NMA510612_RS11050 (NMA510612_2447) pilF 2108950..2110626 (-) 1677 WP_002245855.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NMA510612_RS11060 (NMA510612_2450) - 2111068..2111514 (-) 447 WP_002233503.1 DUF441 domain-containing protein -
  NMA510612_RS11065 (NMA510612_2451) ispB 2111519..2112493 (-) 975 WP_002216396.1 octaprenyl diphosphate synthase -
  NMA510612_RS11070 (NMA510612_2453) rplU 2112717..2113025 (+) 309 WP_002216394.1 50S ribosomal protein L21 -
  NMA510612_RS11075 (NMA510612_2454) rpmA 2113050..2113322 (+) 273 WP_002212328.1 50S ribosomal protein L27 -
  NMA510612_RS15610 - 2113375..2113497 (+) 123 Protein_2080 IS5/IS1182 family transposase -
  NMA510612_RS11080 (NMA510612_2455) ubiM 2113661..2114845 (+) 1185 WP_002239495.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  NMA510612_RS11085 (NMA510612_2456) rpmG 2115116..2115271 (-) 156 WP_002212306.1 50S ribosomal protein L33 -
  NMA510612_RS11090 (NMA510612_2457) rpmB 2115303..2115536 (-) 234 WP_002216391.1 50S ribosomal protein L28 -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61995.07 Da        Isoelectric Point: 5.3037

>NTDB_id=120332 NMA510612_RS11050 WP_002245855.1 2108950..2110626(-) (pilF) [Neisseria meningitidis strain 510612]
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQSLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=120332 NMA510612_RS11050 WP_002245855.1 2108950..2110626(-) (pilF) [Neisseria meningitidis strain 510612]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGAGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAGACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATCCATTTCGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCCTACGGGATGGTGCTGGTAACCGGTCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGAGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGCTTCACCGATGAGGACCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

99.283

100

0.993
  pilB Acinetobacter baumannii D1279779

50.088

100

0.509
  pilB Acinetobacter baylyi ADP1

50.178

100

0.505
  pilB Legionella pneumophila strain ERS1305867

47.35

100

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.457
  pilB Vibrio cholerae strain A1552

45.196

100

0.455
  pilB Vibrio campbellii strain DS40M4

43.662

100

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.771

93.728

0.373

Multiple sequence alignment