Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   CH92_RS02430 Genome accession   NZ_CP007441
Coordinates   519702..520766 (+) Length   354 a.a.
NCBI ID   WP_025240212.1    Uniprot ID   W8RPU0
Organism   Stutzerimonas stutzeri strain 28a24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 514702..525766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH92_RS02420 (CH92_02475) - 515631..516899 (+) 1269 WP_025240210.1 malic enzyme-like NAD(P)-binding protein -
  CH92_RS02425 (CH92_02480) - 517076..519517 (-) 2442 WP_025240211.1 penicillin-binding protein 1A -
  CH92_RS02430 (CH92_02485) comM 519702..520766 (+) 1065 WP_025240212.1 pilus assembly protein PilM Machinery gene
  CH92_RS02435 (CH92_02490) pilN 520766..521338 (+) 573 WP_025240213.1 type 4a pilus biogenesis protein PilN -
  CH92_RS02440 (CH92_02495) pilO 521335..521958 (+) 624 WP_025240214.1 type 4a pilus biogenesis protein PilO -
  CH92_RS02445 (CH92_02500) pilP 521955..522482 (+) 528 WP_025240215.1 type 4a pilus biogenesis lipoprotein PilP -
  CH92_RS02450 (CH92_02505) pilQ 522534..524645 (+) 2112 WP_025240216.1 type IV pilus secretin PilQ Machinery gene
  CH92_RS02455 (CH92_02510) aroK 524677..525195 (+) 519 WP_038622723.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37785.15 Da        Isoelectric Point: 4.5832

>NTDB_id=119341 CH92_RS02430 WP_025240212.1 519702..520766(+) (comM) [Stutzerimonas stutzeri strain 28a24]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYRVESFAVEPLPPNAVVEKNIAELEGVGQALSRLLAKAKTGVKSAAV
AVSGSAVITKSIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGSAARSPGRDEVLLAACRKENVEIREAALAL
AGLTAKVVDVEAYALERSFGLLAPQLGVGHDELTVALVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFHDVDYILLAGGTASIPGLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=119341 CH92_RS02430 WP_025240212.1 519702..520766(+) (comM) [Stutzerimonas stutzeri strain 28a24]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTTGGGATCGACATCAGTTCGACGTCCGTTAAGCTGCTCGAATT
AAGCCGCTCAGGCTCTCGATATCGGGTTGAGTCGTTTGCGGTCGAGCCGCTTCCGCCCAACGCTGTGGTGGAAAAGAACA
TCGCTGAGCTGGAGGGTGTGGGGCAGGCTCTGTCTCGGTTGTTGGCTAAAGCGAAAACCGGAGTCAAGTCCGCCGCAGTT
GCGGTTTCCGGTTCGGCGGTCATCACCAAGAGCATCGAAATGGATGCCGGGCTGAGCGACGATGAGCTGGAAAATCAACT
GAAGATCGAAGCGGATCAGTACATCCCTTACCCGCTGGAAGAGGTTGCTATCGACTTCGAGGTTCAGGGATCCGCCGCAC
GTTCTCCGGGTCGCGACGAGGTGTTGCTGGCGGCATGCCGCAAGGAAAACGTAGAGATCCGCGAGGCGGCTCTGGCCTTG
GCAGGATTGACGGCCAAGGTTGTCGATGTCGAAGCGTATGCGCTGGAACGTTCATTCGGTTTGCTGGCGCCCCAATTGGG
TGTTGGCCACGATGAGCTGACCGTCGCGCTAGTCGATATCGGTGCGACCATGACCACGCTAAGCGTGTTGCATAACGGTC
GCACGATCTATACCCGAGAGCAGCTGTTCGGTGGGCGTCAGCTGACCGAGGAAATTCAGCGTCGCTATGGACTGTCGATG
GAAGAGGCTGGCCTGGCTAAAAAGCAGGGAGGCCTCCCGGACGACTATGACAGCGAAGTATTGCAGCCGTTCAAAGAGGC
AGTCGTTCAGCAGGTGTCGCGCTCGCTGCAGTTCTTCTTTGCGGCCGGGCAGTTTCATGACGTGGACTACATCCTCTTGG
CTGGCGGAACGGCCTCGATCCCGGGGCTGGACCGATTGATTCAGCAGAAAATCGGAACCCAGACTTTAGTCGCCAATCCG
TTTGCCGATATGGCTTTGAGTAGCAAGGTCAACGCTGGCGCGCTCGCCAGTGATGCGCCGTCCCTGATGATTGCCTGCGG
GTTGGCCTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8RPU0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

47.175

100

0.472

Multiple sequence alignment