Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CH92_RS02450 Genome accession   NZ_CP007441
Coordinates   522534..524645 (+) Length   703 a.a.
NCBI ID   WP_025240216.1    Uniprot ID   W8QU14
Organism   Stutzerimonas stutzeri strain 28a24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 517534..529645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH92_RS02430 (CH92_02485) comM 519702..520766 (+) 1065 WP_025240212.1 pilus assembly protein PilM Machinery gene
  CH92_RS02435 (CH92_02490) pilN 520766..521338 (+) 573 WP_025240213.1 type 4a pilus biogenesis protein PilN -
  CH92_RS02440 (CH92_02495) pilO 521335..521958 (+) 624 WP_025240214.1 type 4a pilus biogenesis protein PilO -
  CH92_RS02445 (CH92_02500) pilP 521955..522482 (+) 528 WP_025240215.1 type 4a pilus biogenesis lipoprotein PilP -
  CH92_RS02450 (CH92_02505) pilQ 522534..524645 (+) 2112 WP_025240216.1 type IV pilus secretin PilQ Machinery gene
  CH92_RS02455 (CH92_02510) aroK 524677..525195 (+) 519 WP_038622723.1 shikimate kinase AroK -
  CH92_RS02460 (CH92_02515) aroB 525219..526322 (+) 1104 WP_025240218.1 3-dehydroquinate synthase -
  CH92_RS02465 (CH92_02520) - 526332..527849 (+) 1518 WP_025240219.1 AAA family ATPase -

Sequence

Protein


Download         Length: 703 a.a.        Molecular weight: 75609.61 Da        Isoelectric Point: 4.7980

>NTDB_id=119342 CH92_RS02450 WP_025240216.1 522534..524645(+) (pilQ) [Stutzerimonas stutzeri strain 28a24]
MNTTLSRLGLSLLAAIVSPALLAANLNSLDVASLPGDRVELKMTFDEPVPAPRGYTLDQPARIALDLPGVSSKLTSKNRE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGSNVFVVLGESASATEPVSAASSAPVIPMTKEKLARANAIDNIDF
RRGEDGAGNIVISLSDPSISPSIEEQGGKIRVTFAKTQLPDSLRVRLDVQDFATPVKFVDATSKSGDASISIEPSGRYDY
LAYQADDKLTVSVKPLSEADIEKRKAERFVYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPWD
QALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESKRQIAELAPLRREVVQVNYAKASDIAKLFQTVTNSSGQADD
RGSIAVDDRTNNIIAYQTQERLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKALGVRWGGSFTSGNAAVYGKDGATTF
DSDTGRAYLPGTDTIGNFTASDGVAPVPFVDLGVANSTSGLGIGFITNNLILDLQLSAMEKTGNGEIVSQPKVMTADKET
AKILRGSEIPYQEASSSGATSTSFKEAALSLEVTPLITPDNRVILEVKVTKDAADFSRAVNGVPPINKNEVNAKVLVADG
ETVVIGGVFSNTQTQSVDKVPFLGDLPYVGRLFRRDVVSDAKSELLIFLTPRILNHQAIAVNR

Nucleotide


Download         Length: 2112 bp        

>NTDB_id=119342 CH92_RS02450 WP_025240216.1 522534..524645(+) (pilQ) [Stutzerimonas stutzeri strain 28a24]
ATGAATACCACTCTCTCTCGTCTCGGCTTGTCTTTGCTGGCGGCGATTGTTTCGCCCGCGCTACTGGCCGCAAATCTTAA
TTCCCTCGATGTCGCATCGCTACCCGGAGACCGCGTTGAGCTGAAGATGACGTTCGACGAGCCGGTGCCTGCCCCGCGCG
GATATACGCTCGACCAGCCAGCTCGCATCGCTCTAGATCTGCCAGGTGTTTCCAGCAAGCTGACGTCCAAAAATCGCGAG
CTCGGCGTTGGTAATGCACGGAGTGTGACGGTAGTCGAGGCCCAGGGGCGCACGAGGCTTATCGTCAACCTCACGTCTCT
GGTCCCTTATTCGACCCGAGTCGACGGGAGTAATGTATTTGTCGTGCTTGGCGAAAGCGCTTCTGCTACAGAGCCCGTGT
CCGCAGCTTCGTCGGCGCCAGTCATTCCTATGACGAAGGAAAAGCTGGCCCGGGCGAACGCTATCGACAACATCGACTTC
AGGCGCGGCGAGGATGGCGCAGGCAATATAGTCATAAGCCTGTCTGATCCATCCATCAGTCCGAGCATCGAAGAGCAGGG
TGGGAAGATTCGGGTCACGTTTGCCAAAACGCAGTTGCCCGACTCCTTGCGTGTCCGTCTGGACGTTCAGGACTTCGCAA
CCCCAGTCAAGTTCGTTGACGCAACCAGCAAGTCGGGCGATGCAAGTATCTCTATCGAGCCGAGCGGGCGCTATGACTAT
CTAGCTTATCAGGCTGATGACAAGCTGACCGTCAGCGTCAAGCCACTAAGCGAGGCTGATATCGAAAAGCGTAAAGCAGA
GCGCTTTGTTTATTCGGGTGAGAAGCTGTCACTGAACTTCCAGGACATCGACGTACGCTCCGTGCTGCAGCTAATTGCCG
ACTTCACTGATCTAAATCTTGTTGCCAGTGATACTGTCCAAGGCAATATCACTCTGCGCTTACAAAACGTACCGTGGGAT
CAAGCGCTTGATCTGGTGCTGAAAACTAAGGGGCTCGATAAGCGGCAAGTTGGAAACGTCTTGCTTGTTGCGCCAGCCGA
TGAGATCGCGGCACGTGAAAGGCAAGAATTGGAGTCGAAGCGCCAAATTGCTGAACTTGCGCCGCTACGTCGCGAGGTAG
TTCAGGTGAACTATGCCAAGGCGTCCGACATTGCCAAGCTATTCCAAACGGTTACCAACAGTTCGGGGCAGGCAGATGAT
CGAGGCTCTATCGCTGTTGACGATCGCACAAATAATATAATTGCCTATCAGACTCAAGAGCGCCTTGACGAGCTACGTCG
CATCGTCACTCAATTGGATATTCCGGTCCGCCAAGTAATGATCGAGGCCCGGATTGTGGAGGCCAATGTCGATTATGACA
AAGCGTTAGGCGTGCGTTGGGGGGGGAGTTTCACCTCAGGCAACGCAGCGGTTTATGGTAAGGACGGCGCTACCACCTTT
GATTCAGATACGGGTCGTGCTTATTTGCCAGGAACCGACACTATCGGGAACTTTACCGCTAGTGACGGCGTTGCGCCGGT
GCCCTTCGTTGACTTGGGTGTCGCGAACAGCACCTCTGGCCTCGGCATTGGTTTCATCACTAATAATCTCATCTTAGATC
TGCAGCTTTCGGCAATGGAAAAGACTGGGAACGGGGAAATCGTGTCGCAGCCGAAAGTAATGACTGCCGATAAAGAGACC
GCCAAGATCCTTCGTGGCTCAGAGATTCCATATCAAGAAGCCAGCTCCAGCGGTGCGACATCTACTTCGTTCAAAGAGGC
TGCTCTTTCGCTGGAAGTCACGCCGCTTATCACGCCAGATAATCGCGTGATTCTTGAGGTGAAGGTGACCAAGGACGCGG
CGGATTTCAGTCGTGCCGTAAACGGCGTGCCGCCAATCAACAAGAACGAGGTCAACGCCAAGGTGTTGGTTGCTGATGGC
GAGACCGTAGTTATTGGTGGTGTGTTCTCCAATACGCAAACCCAGTCTGTCGACAAGGTTCCGTTCCTAGGTGACTTGCC
TTACGTAGGGCGGCTGTTCCGGCGCGACGTTGTGTCGGACGCCAAATCTGAGCTGCTGATTTTCCTCACACCGCGTATCC
TGAATCACCAGGCCATTGCAGTGAATCGCTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(299-345)

AMIN domain (AMIN)

(41-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-438)

Bacterial type II and III secretion system protein (Secretin)

(538-694)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8QU14

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.788

100

0.731
  comQ Acinetobacter baylyi ADP1

42.529

99.004

0.421
  pilQ Acinetobacter baumannii strain A118

39.617

100

0.413
  pilQ Acinetobacter baumannii D1279779

39.617

100

0.413

Multiple sequence alignment