Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RG40_RS14360 Genome accession   NZ_CP010150
Coordinates   2775506..2776987 (-) Length   493 a.a.
NCBI ID   WP_073466875.1    Uniprot ID   -
Organism   Escherichia coli strain D7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2770506..2781987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG40_RS14335 (RG40_13660) gspK 2771419..2772402 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  RG40_RS14340 (RG40_13665) gspJ 2772395..2772982 (-) 588 WP_001402582.1 type II secretion system minor pseudopilin GspJ -
  RG40_RS14345 (RG40_13670) gspI 2772975..2773352 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  RG40_RS14350 (RG40_13675) gspH 2773349..2773858 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  RG40_RS25240 (RG40_13680) gspG 2773866..2774303 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  RG40_RS14355 (RG40_13685) gspF 2774313..2775509 (-) 1197 WP_001109573.1 type II secretion system inner membrane protein GspF -
  RG40_RS14360 (RG40_13690) pilB 2775506..2776987 (-) 1482 WP_073466875.1 type II secretion system ATPase GspE Machinery gene
  RG40_RS14365 (RG40_13695) gspD 2776997..2778949 (-) 1953 WP_023155400.1 type II secretion system secretin GspD -
  RG40_RS14370 (RG40_13700) gspC 2778933..2779748 (-) 816 WP_001142708.1 type II secretion system protein GspC -
  RG40_RS14375 (RG40_13705) gspA 2779928..2781397 (+) 1470 WP_001402579.1 type II secretion system protein GspA -
  RG40_RS14380 (RG40_13710) gspB 2781399..2781818 (+) 420 WP_000461449.1 putative general secretion pathway protein GspB -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54582.61 Da        Isoelectric Point: 6.3748

>NTDB_id=116266 RG40_RS14360 WP_073466875.1 2775506..2776987(-) (pilB) [Escherichia coli strain D7]
MRIHSPYPASWALAQRIGYLYSEGEIIYLAGTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILMVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDE
VMRVASATLESEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=116266 RG40_RS14360 WP_073466875.1 2775506..2776987(-) (pilB) [Escherichia coli strain D7]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGGTACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCTTGGTGACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGATGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGCCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTAGTCCGGCAACAACACAAGGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAGCGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.453

88.641

0.412
  pilB Vibrio cholerae strain A1552

48.201

84.584

0.408
  pilB Acinetobacter baumannii D1279779

45.351

89.452

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

46.118

86.207

0.398
  pilB/pilB1 Synechocystis sp. PCC 6803

40

99.391

0.398
  pilB Legionella pneumophila strain ERS1305867

40.748

97.566

0.398
  pilB Acinetobacter baylyi ADP1

48.082

79.31

0.381
  pilF Thermus thermophilus HB27

45.063

80.122

0.361

Multiple sequence alignment