Detailed information NTG1158501

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QWJ11_RS13090
• Genome accession: NZ_OX458335
• Coordinates: 2806178..2807347 (+)
• Length: 389 a.a.
• NCBI ID: WP_007246444.1
• Uniprot ID: A0AAW4DX87
• Organism: Pseudomonas syringae pv. tomato strain DAPP-PG 215 isolate IPVPG 215
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2801178..2812347

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QWJ11_RS13075 (DAPPPG215_13205)	-	2801641..2803926 (+)	2286	WP_007246442.1	ATP-dependent DNA helicase	-
QWJ11_RS13080 (DAPPPG215_13210)	pgm	2804027..2805673 (+)	1647	WP_007246443.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
QWJ11_RS13085 (DAPPPG215_13215)	-	2805748..2806023 (-)	276	WP_003373489.1	peptidylprolyl isomerase	-
QWJ11_RS13090 (DAPPPG215_13220)	pilU	2806178..2807347 (+)	1170	WP_007246444.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QWJ11_RS13095 (DAPPPG215_13225)	-	2807526..2809103 (-)	1578	WP_007246445.1	ABC transporter ATP-binding protein	-
QWJ11_RS13100 (DAPPPG215_13230)	-	2809105..2810127 (-)	1023	WP_007246446.1	ABC transporter permease	-
QWJ11_RS13105 (DAPPPG215_13235)	-	2810127..2811191 (-)	1065	WP_007246447.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42682.80 Da
• Isoelectric Point: 5.8663

>NTDB_id=1158501 QWJ11_RS13090 WP_007246444.1 2806178..2807347(+) (pilU) [Pseudomonas syringae pv. tomato strain DAPP-PG 215 isolate IPVPG 215]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLSSETLKPGEVAVVAQGLMDEEQKLEFVRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIINQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVDGAISEEEALKNADSQNNVRLRLKLHSENGVANLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=1158501 QWJ11_RS13090 WP_007246444.1 2806178..2807347(+) (pilU) [Pseudomonas syringae pv. tomato strain DAPP-PG 215 isolate IPVPG 215]
ATGGATTTTCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCTGACCTTTATCTTTCTACCGGAGCACCGCCGTG
TGCCAAGTTCAACGGCGTGCTCAAGCCGCTGAGCAGCGAAACCCTCAAGCCTGGCGAAGTGGCGGTGGTTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAGTTTGTCCGGGAACTGGAGATGAACCTCGCGGTGTCATTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCTATTGTCGCGCGTAACATCAAGCTGGACATTCCGCGCTTTGA
GGACCTTTTCCTGCCGCCGGTATTGCTTGACGTGATCATGGAAAAACACGGTCTGGTGCTGTTTGTCGGCGCCACTGGCT
CGGGTAAATCGACCTCGCTGGCGGCGCTGATTGATTACCGTAACCGCAATGCCAGCGGGCACATCATCACCATTGAGGAC
CCGGTCGAATTTATCCATCGGCACAAGAAGTCCATCATCAATCAGCGTGAGGTCGGCGTCGACACCCGCAGCTTTCGCGC
GGCACTGAAAAACACTCTGCGTCAGGCCCCGGATGTGATTCTGATCGGCGAGATCCGCGATCGCGAAACGATGGAGCACG
CGCTGGCATTTGCCGACACCGGCCACTTGGCGATTTCGACCCTGCACGCCAATAACGCCAACCAGGCGCTGGACCGAATC
ATCAACTTTTTCCCGGAAGAGCGGCGTGCGCAGCTGTTGCACGACCTGGGCAACAACCTCAAGGCGTTCGTGTCTCAGCG
GCTGGTAAAGACGCCTGATGGAAAACGGCGCGCGGCGGTGGAAGTGATGATGGGCACGCCGACCATCCGCGACCTGATCC
AGCGTAATGAGCTGACTGAACTCAAGGGCATCATGGAAAAGTCCGGCAGCCTCGGGATGCAGACGTTCGATACCGCGCTG
TTCAACCTGGCCGTCGATGGCGCAATCAGCGAGGAAGAAGCGTTGAAAAATGCCGATTCGCAAAACAACGTTCGTCTGCG
CCTCAAGCTGCACAGCGAGAACGGAGTGGCTAACCTGACCACGCCGCCCCCGGCACCGACCGGCAGCAGCACAGCCAGCA
CCGCCGAGTGGGGCCTGGTCGACGATGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.814	99.486	0.555
pilU	Acinetobacter baylyi ADP1	52.394	91.26	0.478
pilU	Vibrio cholerae strain A1552	51.143	89.974	0.46
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.426	98.458	0.388
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362