Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   KJP45_RS12245 Genome accession   NZ_OX419578
Coordinates   2392116..2392499 (-) Length   127 a.a.
NCBI ID   WP_080529536.1    Uniprot ID   -
Organism   Bacillus subtilis isolate NRS6128     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2387116..2397499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP45_RS12205 (NRS6128_12110) sinI 2388050..2388223 (+) 174 WP_003230187.1 anti-repressor SinI family protein Regulator
  KJP45_RS12210 (NRS6128_12115) sinR 2388257..2388592 (+) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  KJP45_RS12215 (NRS6128_12120) tasA 2388685..2389470 (-) 786 WP_003230183.1 biofilm matrix protein TasA -
  KJP45_RS12220 (NRS6128_12125) sipW 2389534..2390106 (-) 573 WP_003230181.1 signal peptidase I -
  KJP45_RS12225 (NRS6128_12130) tapA 2390090..2390851 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  KJP45_RS12230 (NRS6128_12135) yqzG 2391123..2391449 (+) 327 WP_029317915.1 YqzG/YhdC family protein -
  KJP45_RS12235 (NRS6128_12140) spoIIT 2391491..2391670 (-) 180 WP_213414620.1 YqzE family protein -
  KJP45_RS12240 (NRS6128_12145) comGG 2391741..2392115 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  KJP45_RS12245 comGF 2392116..2392499 (-) 384 WP_080529536.1 ComG operon protein ComGF Machinery gene
  KJP45_RS12250 (NRS6128_12155) comGE 2392525..2392872 (-) 348 WP_080529537.1 ComG operon protein 5 Machinery gene
  KJP45_RS12255 (NRS6128_12160) comGD 2392856..2393287 (-) 432 WP_080529538.1 comG operon protein ComGD Machinery gene
  KJP45_RS12260 (NRS6128_12165) comGC 2393277..2393573 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  KJP45_RS12265 (NRS6128_12170) comGB 2393587..2394624 (-) 1038 WP_213414619.1 comG operon protein ComGB Machinery gene
  KJP45_RS12270 (NRS6128_12175) comGA 2394611..2395681 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  KJP45_RS12275 (NRS6128_12180) - 2395893..2396090 (-) 198 WP_213414618.1 hypothetical protein -
  KJP45_RS12280 (NRS6128_12185) corA 2396092..2397045 (-) 954 WP_004399136.1 magnesium transporter CorA -

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14414.53 Da        Isoelectric Point: 6.2135

>NTDB_id=1157829 KJP45_RS12245 WP_080529536.1 2392116..2392499(-) (comGF) [Bacillus subtilis isolate NRS6128]
MLISGSLAAIFHLFLSRQQEHDGFTQQEWMISIEQMMNECKESQAVKTAEHGSVLICTNLSGQDIRFDIYHSMIRKRVDG
KGHVPILDHITAMKADIENGVVLLKIESEDQKVYQTAFPVYSYLGGR

Nucleotide


Download         Length: 384 bp        

>NTDB_id=1157829 KJP45_RS12245 WP_080529536.1 2392116..2392499(-) (comGF) [Bacillus subtilis isolate NRS6128]
TTGCTCATATCAGGATCGTTAGCTGCGATTTTCCATCTGTTTTTGTCTCGACAGCAGGAACATGACGGTTTCACACAGCA
AGAATGGATGATTTCTATAGAACAGATGATGAATGAATGCAAGGAGTCACAGGCAGTTAAGACAGCCGAGCATGGGAGCG
TGTTAATCTGCACCAATCTTTCCGGACAAGACATCCGTTTTGACATTTATCATTCAATGATAAGAAAAAGAGTGGATGGC
AAAGGGCATGTTCCGATTTTAGATCATATTACTGCCATGAAAGCTGATATTGAAAATGGTGTTGTTTTGCTGAAAATTGA
GAGTGAGGACCAAAAAGTGTATCAAACTGCTTTTCCAGTCTATTCGTATTTAGGAGGGAGGTGA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(16-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Bacillus subtilis subsp. subtilis str. 168

99.206

99.213

0.984

Multiple sequence alignment