Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGA   Type   Machinery gene
Locus tag   KJP45_RS12270 Genome accession   NZ_OX419578
Coordinates   2394611..2395681 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis isolate NRS6128     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2389611..2400681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP45_RS12225 (NRS6128_12130) tapA 2390090..2390851 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  KJP45_RS12230 (NRS6128_12135) yqzG 2391123..2391449 (+) 327 WP_029317915.1 YqzG/YhdC family protein -
  KJP45_RS12235 (NRS6128_12140) spoIIT 2391491..2391670 (-) 180 WP_213414620.1 YqzE family protein -
  KJP45_RS12240 (NRS6128_12145) comGG 2391741..2392115 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  KJP45_RS12245 comGF 2392116..2392499 (-) 384 WP_080529536.1 ComG operon protein ComGF Machinery gene
  KJP45_RS12250 (NRS6128_12155) comGE 2392525..2392872 (-) 348 WP_080529537.1 ComG operon protein 5 Machinery gene
  KJP45_RS12255 (NRS6128_12160) comGD 2392856..2393287 (-) 432 WP_080529538.1 comG operon protein ComGD Machinery gene
  KJP45_RS12260 (NRS6128_12165) comGC 2393277..2393573 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  KJP45_RS12265 (NRS6128_12170) comGB 2393587..2394624 (-) 1038 WP_213414619.1 comG operon protein ComGB Machinery gene
  KJP45_RS12270 (NRS6128_12175) comGA 2394611..2395681 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  KJP45_RS12275 (NRS6128_12180) - 2395893..2396090 (-) 198 WP_213414618.1 hypothetical protein -
  KJP45_RS12280 (NRS6128_12185) corA 2396092..2397045 (-) 954 WP_004399136.1 magnesium transporter CorA -
  KJP45_RS12285 (NRS6128_12190) yqhB 2397188..2398513 (+) 1326 WP_213414617.1 hemolysin family protein -
  KJP45_RS12290 (NRS6128_12195) rsbRD 2398570..2399406 (-) 837 WP_021480027.1 RsbT co-antagonist protein RsbRD -
  KJP45_RS12300 (NRS6128_12205) mgsR 2399630..2400010 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  KJP45_RS12305 (NRS6128_12210) yqgY 2400242..2400487 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence


Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=1157834 KJP45_RS12270 WP_004399124.1 2394611..2395681(-) (comGA) [Bacillus subtilis isolate NRS6128]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1157834 KJP45_RS12270 WP_004399124.1 2394611..2395681(-) (comGA) [Bacillus subtilis isolate NRS6128]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGAGGGCAGCGATGACGGGACATCTGGTACTGACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1


Multiple sequence alignment