Detailed information NTG0115716

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N018_RS11755
• Genome accession: NZ_CP007014
• Coordinates: 2578139..2579308 (+)
• Length: 389 a.a.
• NCBI ID: WP_025389668.1
• Uniprot ID: W0MVU8
• Organism: Pseudomonas syringae CC1557
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2573139..2584308

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N018_RS11740 (N018_11955)	-	2573586..2575841 (+)	2256	WP_025389667.1	ATP-dependent DNA helicase	-
N018_RS11745 (N018_11960)	pgm	2575969..2577615 (+)	1647	WP_024646290.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
N018_RS11750 (N018_11965)	-	2577722..2577997 (-)	276	WP_024646289.1	peptidylprolyl isomerase	-
N018_RS11755 (N018_11975)	pilU	2578139..2579308 (+)	1170	WP_025389668.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
N018_RS11760 (N018_11985)	-	2579494..2581071 (-)	1578	WP_025389669.1	ABC transporter ATP-binding protein	-
N018_RS11765 (N018_11990)	-	2581073..2582095 (-)	1023	WP_025389670.1	ABC transporter permease	-
N018_RS11770 (N018_11995)	-	2582095..2583159 (-)	1065	WP_024645869.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42757.92 Da
• Isoelectric Point: 6.0662

>NTDB_id=115716 N018_RS11755 WP_025389668.1 2578139..2579308(+) (pilU) [Pseudomonas syringae CC1557]
MDFPALLKILANQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGDVALIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEELFLPPVLLDVVMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLVISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSASLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAATLTTPPPAPTGSSTANTAEWGLVDDDERGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=115716 N018_RS11755 WP_025389668.1 2578139..2579308(+) (pilU) [Pseudomonas syringae CC1557]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAATCAGGACGGATCGGACCTCTATCTTTCCACCGGAGCACCGCCGTG
CGCCAAGTTCAATGGCGTGCTCAAGCCACTCGGCAGCGAGACGTTCAAACCCGGCGATGTGGCGTTGATTGCCCAAGGGC
TGATGGACGAAGAGCAGAAGCTCGAATTTCTGCGTGAACTGGAGATGAACCTTGCGGTGTCGCTGGCGGGCATCGGTCGC
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCTATTGTTGCCCGTAACATCAAGCTGGACATCCCGCGCTTCGA
GGAGCTTTTCCTGCCGCCTGTGTTACTCGATGTGGTCATGGAAAAGCACGGTCTGGTGCTGTTCGTCGGTGCAACCGGTT
CGGGTAAGTCGACCTCGCTGGCAGCCCTGATCGATTACCGAAATCGCAACGCCAGCGGGCACATCATCACCATTGAAGAC
CCGGTGGAGTTTATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAAGTCGGCGTCGATACCCGCAGCTTCCGCGC
CGCGTTGAAGAACACCTTGCGCCAGGCGCCGGATGTGATTCTGATCGGCGAAATTCGTGATCGCGAAACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGACACTTGGTGATCTCGACCCTGCATGCCAACAACGCCAATCAGGCACTGGACAGGATC
ATCAACTTTTTCCCGGAAGAGCGGCGCGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTTGTTTCCCAGCG
GCTGGTCAAAACGCCGGATGGCAAGCGTCGGGCGGCGGTGGAAGTGATGATGGGCACGCCGACCATTCGCGATCTGATTC
AGCGCAACGAACTGACCGAACTCAAGGGCATCATGGAAAAATCCGCCAGCCTTGGTATGCAGACCTTTGACTCGGCCTTG
TTCAATCTGGCAGTGGAAGGGGCGATCAGCGAAGAGGAAGCGTTGAAGAATGCCGACTCGCAGAACAACGTACGGCTGCG
CCTCAAGCTTCACAGCGAAGGCGGAGCTGCGACCCTGACCACGCCGCCCCCGGCACCGACCGGCAGCAGCACCGCAAACA
CGGCGGAGTGGGGGCTGGTCGATGACGACGAGCGGGGCCCGCAGGCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-248)

3D structure

• AlphaFold DB: W0MVU8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.735	93.059	0.537
pilU	Acinetobacter baylyi ADP1	50.98	91.774	0.468
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.948	98.458	0.393
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365