Detailed information NTG1154244

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Q0V31_RS16545
• Genome accession: NZ_OX044366
• Coordinates: 3535065..3536285 (-)
• Length: 406 a.a.
• NCBI ID: WP_298189486.1
• Uniprot ID: -
• Organism: MAG: uncultured Pseudomonas sp. isolate AG_MB4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3530065..3541285

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q0V31_RS16530	-	3530835..3531602 (+)	768	WP_298189478.1	3-hydroxybutyrate dehydrogenase	-
Q0V31_RS16535	-	3531636..3532982 (+)	1347	WP_298189481.1	TIGR00366 family protein	-
Q0V31_RS16540	-	3532995..3534950 (+)	1956	WP_298189483.1	acetoacetate--CoA ligase	-
Q0V31_RS16545	pilU	3535065..3536285 (-)	1221	WP_298189486.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Q0V31_RS16550	-	3536428..3536706 (+)	279	WP_090253882.1	peptidylprolyl isomerase	-
Q0V31_RS16555	-	3536710..3537057 (+)	348	WP_298189487.1	MmcQ/YjbR family DNA-binding protein	-
Q0V31_RS16560	wrbA	3537271..3537870 (+)	600	WP_298189489.1	NAD(P)H:quinone oxidoreductase	-
Q0V31_RS16565	-	3537888..3538184 (-)	297	Protein_3256	hypothetical protein	-
Q0V31_RS16570	-	3538363..3538569 (+)	207	Protein_3257	site-specific integrase	-
Q0V31_RS16575	-	3538582..3540168 (+)	1587	WP_298189492.1	ABC-F family ATPase	-
Q0V31_RS16580	-	3540243..3541208 (-)	966	WP_298189495.1	alpha/beta hydrolase	-

Sequence

Protein

• Length: 406 a.a.
• Molecular weight: 44468.87 Da
• Isoelectric Point: 5.7533

>NTDB_id=1154244 Q0V31_RS16545 WP_298189486.1 3535065..3536285(-) (pilU) [MAG: uncultured Pseudomonas sp. isolate AG_MB4]
MDLNAMLKVLSSQDGSDLYLSTGAPPCAKFNGVLKALSAEPLKAGEVAEIADAVMDVAQREEFERELEMNLAISIAGVGR
FRINIFKQRNEVSIVARNIKLDIPKFEDLKLPEVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHKHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVKTVDGKRRAAVEVLLGTPTIRDLIKRNEFAEIKEIMEKSKNLGMQTFDQAL
IDLVNDGSIDEEEAVKNADSANNVRLKLKLYRETPSAPAVVAAPAPAATAARPAAPAAPAGDWGLELKLEDIEVEQPPED
PGRNGI

Nucleotide

• Length: 1221 bp

>NTDB_id=1154244 Q0V31_RS16545 WP_298189486.1 3535065..3536285(-) (pilU) [MAG: uncultured Pseudomonas sp. isolate AG_MB4]
ATGGACCTCAATGCAATGCTCAAAGTCCTGTCCAGTCAGGACGGGTCCGATCTTTACCTGTCAACGGGTGCGCCGCCCTG
CGCCAAGTTCAACGGCGTGCTCAAGGCGCTGAGCGCCGAGCCACTCAAGGCCGGTGAGGTGGCCGAGATCGCTGATGCGG
TGATGGATGTGGCGCAGCGTGAAGAATTTGAACGTGAGCTGGAGATGAACCTGGCCATCTCGATTGCCGGCGTCGGGCGT
TTCCGCATAAACATTTTTAAGCAGCGCAACGAGGTGTCGATCGTCGCGCGCAATATCAAGCTGGATATCCCCAAGTTTGA
AGACCTCAAACTGCCGGAAGTACTGCTCAAGACGGTGATGGAGAAGCGCGGTCTGGTGCTCTTTGTCGGTGGCACTGGCT
CGGGTAAATCGACCTCACTGGCAGCCTTGATCGACTACCGCAACCGCAACAGCGGTGGCCATATCATCACCATCGAAGAC
CCGGTGGAGTATGTGCATAAGCACAAAAAATCGATCATCAATCAACGTGAAGTCGGGGTCGATACCCGCAGCTTTCATGC
AGCGCTGAAAAACACCCTGCGCCAGGCGCCTGATGTGATTTTGATTGGCGAAATCCGTGACCGCGAGACCATGGAGCACG
CCCTGGCGTTTGCCGACACCGGGCACTTGGCAATTTCCACGTTGCACGCCAACAACGCCAACCAGGCGTTGGACCGCATC
ATCAACTTCTTTCCCGAAGACCGTCGGCCGCAGCTGCTTAACGACTTGGGCAACAATCTCAAGGCGTTCGTTTCCCAGCG
GCTGGTGAAGACGGTCGACGGCAAGCGCCGCGCGGCGGTTGAAGTGCTGCTGGGTACGCCGACCATTCGTGACCTGATCA
AACGCAACGAGTTTGCCGAGATCAAGGAGATCATGGAGAAATCGAAGAACCTCGGCATGCAGACCTTCGATCAGGCCCTG
ATCGATCTGGTTAATGACGGTTCGATTGATGAAGAGGAAGCGGTGAAAAACGCCGATTCTGCAAACAACGTCCGCCTCAA
GCTCAAGCTCTATCGCGAGACACCAAGCGCGCCGGCCGTCGTCGCAGCCCCTGCTCCGGCTGCCACTGCAGCGCGCCCAG
CAGCACCAGCAGCGCCAGCGGGTGACTGGGGGCTTGAGTTGAAGTTGGAAGATATTGAAGTCGAGCAGCCGCCGGAAGAT
CCGGGCCGCAATGGGATTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.901	87.438	0.498
pilU	Acinetobacter baylyi ADP1	52.394	87.438	0.458
pilU	Vibrio cholerae strain A1552	52.408	86.946	0.456
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.52	96.552	0.372