Detailed information NTG0115347

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BAMY6614_RS15390
• Genome accession: NZ_CP006960
• Coordinates: 3246577..3247191 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus amyloliquefaciens UMAF6614
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3241577..3252191

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAMY6614_RS15370 (BAMY6614_16235)	rpsT	3241880..3242146 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BAMY6614_RS15375 (BAMY6614_16240)	holA	3242163..3243204 (-)	1042	Protein_3064	DNA polymerase III subunit delta	-
BAMY6614_RS20075	-	3243244..3243395 (-)	152	Protein_3065	hypothetical protein	-
BAMY6614_RS19660 (BAMY6614_16245)	-	3243436..3243570 (+)	135	WP_003152870.1	YqzM family protein	-
BAMY6614_RS15380 (BAMY6614_16250)	comEC	3243589..3245940 (-)	2352	WP_022552933.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BAMY6614_RS15385 (BAMY6614_16255)	-	3245941..3246510 (-)	570	WP_003152868.1	ComE operon protein 2	-
BAMY6614_RS15390 (BAMY6614_16260)	comEA	3246577..3247191 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
BAMY6614_RS15395 (BAMY6614_16265)	comER	3247250..3248071 (+)	822	WP_061862409.1	late competence protein ComER	-
BAMY6614_RS15400 (BAMY6614_16270)	-	3248140..3248877 (-)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
BAMY6614_RS15405 (BAMY6614_16275)	rsfS	3248874..3249230 (-)	357	WP_007408260.1	ribosome silencing factor	-
BAMY6614_RS15410 (BAMY6614_16280)	yqeK	3249248..3249808 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BAMY6614_RS15415 (BAMY6614_16285)	-	3249798..3250367 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
BAMY6614_RS15420 (BAMY6614_16290)	yhbY	3250378..3250668 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BAMY6614_RS15425 (BAMY6614_16295)	aroE	3250662..3251504 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=115347 BAMY6614_RS15390 WP_014418419.1 3246577..3247191(-) (comEA) [Bacillus amyloliquefaciens UMAF6614]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=115347 BAMY6614_RS15390 WP_014418419.1 3246577..3247191(-) (comEA) [Bacillus amyloliquefaciens UMAF6614]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363