Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L1X57_RS14475 Genome accession   NZ_OV350343
Coordinates   3241223..3242977 (-) Length   584 a.a.
NCBI ID   WP_234667767.1    Uniprot ID   A0AA35E400
Organism   Halomonas sp. TD01 strain Halomonas Bluephagenesis     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3236223..3247977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1X57_RS14445 (HPTD01_2979) - 3236789..3237133 (+) 345 WP_009724764.1 hypothetical protein -
  L1X57_RS14450 (HPTD01_2980) - 3237165..3238037 (-) 873 WP_009724765.1 hypothetical protein -
  L1X57_RS14455 (HPTD01_2981) yacG 3238145..3238378 (-) 234 WP_009724766.1 DNA gyrase inhibitor YacG -
  L1X57_RS14460 (HPTD01_2982) coaE 3238441..3239037 (-) 597 WP_009724767.1 dephospho-CoA kinase -
  L1X57_RS14465 (HPTD01_2983) pilD 3239078..3239923 (-) 846 WP_009724768.1 A24 family peptidase Machinery gene
  L1X57_RS14470 (HPTD01_2984) - 3239985..3241214 (-) 1230 WP_009724769.1 type II secretion system F family protein -
  L1X57_RS14475 (HPTD01_2985) pilB 3241223..3242977 (-) 1755 WP_234667767.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L1X57_RS18860 (HPTD01_2987) - 3243214..3243654 (+) 441 WP_009724771.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  L1X57_RS18865 (HPTD01_2988) - 3243737..3244129 (+) 393 WP_009724772.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  L1X57_RS14490 (HPTD01_2989) - 3244222..3244677 (+) 456 WP_009724773.1 anti-virulence regulator CigR family protein -
  L1X57_RS14495 (HPTD01_2990) - 3244773..3245582 (+) 810 WP_009724774.1 inositol monophosphatase family protein -
  L1X57_RS14505 (HPTD01_2991) - 3245894..3247033 (-) 1140 WP_009724555.1 tyrosine-type recombinase/integrase -
  L1X57_RS14510 (HPTD01_2992) - 3247035..3247238 (-) 204 WP_009724554.1 helix-turn-helix domain-containing protein -
  L1X57_RS14515 (HPTD01_2993) - 3247240..3247752 (-) 513 WP_009724553.1 hypothetical protein -

Sequence

Protein


Download         Length: 584 a.a.        Molecular weight: 63679.10 Da        Isoelectric Point: 6.5091

>NTDB_id=1151847 L1X57_RS14475 WP_234667767.1 3241223..3242977(-) (pilB) [Halomonas sp. TD01 strain Halomonas Bluephagenesis]
MSQPAPESTLSHAALNGGLRGIAKRLVKHGVLTDAQATVAESTAAELEVSLLQHVVDSGLVESGAAALAAAWEYGLPVVD
LDAIRINALPPANDYPVKILERLDILPLARHGHRLTVAVPYPATLTQLDELQFATGLSVDGVLAPADQLKNTLTQYLAQN
ERSMLDELENVDDAVSALNIVHLGEDEATFDKAVKDSEDAPVVKFVNKILLDAISRGASDIHFEPYETQYRVRFRIDGML
IEIARPPFALRHRIAARLKIMSRLDISERRLPQDGAIKLQLSRTRSIDFRVNSLPTVFGEKIVLRLLDPASAQLGIEQLG
FSPEQQIAYEHALKKPQGMILVTGPTGSGKTVTLYTGINILNQIERNICTAEDPVEIKVSGINQVNILPKIGLNFASALR
AFLRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGVASYNIASSVSLIIAQRLARKLCNLC
KTPAKIPAEALRKAGFSQQDIRNGTIYSAVGCKQCTQGYKGRIGIYEVVPITDAMRQLIMRDGDALDIDQQARREGYPSL
HQRCLSRVLDGSTSLEEVNRISKE

Nucleotide


Download         Length: 1755 bp        

>NTDB_id=1151847 L1X57_RS14475 WP_234667767.1 3241223..3242977(-) (pilB) [Halomonas sp. TD01 strain Halomonas Bluephagenesis]
ATGAGCCAACCTGCCCCAGAGTCTACGCTGAGCCATGCGGCCTTAAATGGCGGCCTTCGTGGTATCGCTAAACGGCTGGT
GAAACATGGCGTACTGACCGACGCTCAGGCGACTGTCGCGGAGAGCACGGCTGCAGAGCTTGAAGTGTCGCTGTTACAGC
ACGTGGTTGATAGTGGGTTAGTGGAATCAGGCGCGGCAGCGCTGGCCGCTGCCTGGGAGTATGGTTTACCGGTTGTTGAT
CTAGATGCCATTCGTATTAACGCACTCCCCCCCGCCAACGACTATCCCGTTAAAATATTAGAGCGTCTGGATATTCTTCC
CCTGGCGCGTCACGGGCATCGCCTTACCGTGGCGGTGCCCTATCCCGCCACACTTACCCAACTGGATGAGCTGCAGTTTG
CTACGGGGCTAAGTGTAGATGGCGTGCTAGCGCCTGCTGATCAACTTAAAAATACCCTGACGCAATACCTGGCGCAAAAT
GAACGCAGCATGCTGGATGAGCTGGAAAACGTTGATGATGCGGTCAGCGCGCTAAATATTGTTCATCTTGGCGAAGATGA
GGCAACGTTTGACAAGGCGGTAAAAGATAGCGAAGACGCCCCCGTCGTTAAATTCGTTAATAAAATACTGCTAGATGCTA
TCAGCCGTGGCGCTTCCGATATTCACTTTGAGCCTTATGAAACCCAGTATCGAGTGCGCTTCCGCATCGATGGCATGCTG
ATTGAAATTGCTCGCCCTCCTTTTGCGCTCCGACACCGCATTGCTGCACGTTTGAAAATCATGTCTCGGCTGGATATTTC
TGAGCGCCGCCTGCCTCAGGATGGCGCAATCAAGCTACAGCTTTCACGCACCCGATCAATTGACTTCCGGGTGAACTCAC
TGCCCACCGTATTCGGTGAGAAAATAGTACTGCGCCTGTTAGACCCCGCCTCTGCTCAGCTGGGCATTGAGCAGCTAGGC
TTTAGTCCTGAACAGCAGATCGCCTATGAGCATGCGCTTAAGAAGCCTCAGGGAATGATTCTGGTTACCGGGCCAACAGG
TAGCGGTAAAACAGTGACGCTCTACACAGGCATCAATATTCTCAACCAAATTGAGCGCAATATCTGCACTGCAGAAGACC
CGGTAGAAATTAAGGTGTCGGGAATCAATCAAGTTAACATTCTCCCTAAAATCGGCCTAAACTTCGCCAGCGCTCTACGC
GCCTTTTTACGCCAAGACCCTGACGTCGTCATGGTTGGCGAAATTCGCGACTTGGAAACCGCCGAAATTGCCGTAAAGGC
AGCCCAAACAGGCCACTTAGTGCTATCTACCGTACACACTAACTCAGCCGCTGAAACGCTAACCCGGTTAGCGAACATGG
GCGTCGCGTCCTATAACATCGCAAGCTCAGTTAGCCTGATCATTGCCCAGCGCTTGGCACGTAAACTATGCAACCTGTGT
AAAACACCCGCTAAGATTCCGGCCGAAGCGCTGCGTAAGGCTGGCTTTAGCCAGCAGGATATCCGCAACGGCACAATTTA
CTCAGCGGTCGGCTGCAAACAGTGCACCCAGGGCTACAAAGGGCGAATTGGTATTTATGAGGTGGTGCCCATTACCGATG
CCATGCGCCAGTTGATTATGCGCGATGGTGATGCGCTGGATATTGATCAGCAAGCCCGCCGCGAAGGCTACCCTAGCCTT
CATCAGCGTTGCTTAAGTCGTGTGCTGGATGGCAGCACTAGCCTAGAAGAAGTTAACCGCATCAGTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(208-475)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-161)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

52.92

96.747

0.512
  pilB Acinetobacter baylyi ADP1

49.823

96.918

0.483
  pilB Acinetobacter baumannii D1279779

49.293

96.918

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

48.399

96.233

0.466
  pilB Vibrio campbellii strain DS40M4

47.544

97.603

0.464
  pilB Vibrio cholerae strain A1552

51.852

87.842

0.455
  pilF Neisseria gonorrhoeae MS11

49.476

81.678

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.071

100

0.385

Multiple sequence alignment