Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   LWS60_RS20995 Genome accession   NZ_OU701452
Coordinates   4356400..4357920 (-) Length   506 a.a.
NCBI ID   WP_001551955.1    Uniprot ID   A0A0D8WAL6
Organism   Escherichia coli isolate CNR65D6     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 4351400..4362920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LWS60_RS20985 hdfR 4355078..4355917 (-) 840 WP_000379230.1 HTH-type transcriptional regulator HdfR -
  LWS60_RS20990 maoP 4356036..4356374 (+) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  LWS60_RS20995 comM 4356400..4357920 (-) 1521 WP_001551955.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  LWS60_RS21000 ilvL 4358273..4358371 (+) 99 WP_001311244.1 ilv operon leader peptide -
  LWS60_RS21005 ilvX 4358458..4358508 (+) 51 WP_001387183.1 peptide IlvX -
  LWS60_RS21010 ilvG 4358511..4360157 (+) 1647 WP_001551957.1 acetolactate synthase 2 catalytic subunit -
  LWS60_RS21015 ilvM 4360154..4360417 (+) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  LWS60_RS21020 ilvE 4360437..4361366 (+) 930 WP_000208520.1 branched-chain-amino-acid transaminase -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55227.41 Da        Isoelectric Point: 7.8952

>NTDB_id=1151527 LWS60_RS20995 WP_001551955.1 4356400..4357920(-) (comM) [Escherichia coli isolate CNR65D6]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ESSATVKQRVMAARKRQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=1151527 LWS60_RS20995 WP_001551955.1 4356400..4357920(-) (comM) [Escherichia coli isolate CNR65D6]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCCGGCTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCCCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAATCTGGCTCCAGCCGATCTGCCAAAAGAAGGGGGACGA
TATGATTTACCTATCGCCATTGCGTTGCTGGCGGCCTCAGAACAGCTTACAGCCAATAAATTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGCGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAGGCTGTCTGTGCGTTTCTGGAGGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGGCACAACCTTT
TACTGATTGGGCCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTGTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCGTCGTTAACTGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCAGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGCTACCTGAATTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAACTTGTTGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACACCAGAGCAAACGCTGCGTTATCTCAACCGACTCTCTGGTC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCGCCACCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAGCAGCGCCACCGTTAAACAACGCGTAATGGCCGCCAGAAAGCGCCAATTTAAGCGGCAGAATAAACTGAACGCCTG
GCTGGATAATCCGGAAATACGCCAATTCTGCAAGCTTGAGAGCGAAGATGCGCAGTGGTTGGAAGAAACGCTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCCGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGTGCCATTGACCGTTTGCTCATCCATCTGCAAAAACTACTGACATA
A

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D8WAL6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.317

100

0.589
  comM Glaesserella parasuis strain SC1401

58.58

100

0.587
  comM Vibrio cholerae strain A1552

58.964

99.209

0.585
  comM Vibrio campbellii strain DS40M4

57.968

99.209

0.575
  comM Legionella pneumophila str. Paris

47.791

98.419

0.47
  comM Legionella pneumophila strain ERS1305867

47.791

98.419

0.47
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.071

100

0.441

Multiple sequence alignment