Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   X874_RS03165 Genome accession   NZ_CP006944
Coordinates   656003..657316 (-) Length   437 a.a.
NCBI ID   WP_025247435.1    Uniprot ID   -
Organism   Mannheimia varigena USDA-ARS-USMARC-1312     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 651003..662316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  X874_RS03135 (X874_6180) ruvB 651631..652635 (-) 1005 WP_025247430.1 Holliday junction branch migration DNA helicase RuvB -
  X874_RS03140 (X874_6190) ruvA 652646..653251 (-) 606 WP_025216876.1 Holliday junction branch migration protein RuvA -
  X874_RS03145 (X874_6200) - 653360..653986 (-) 627 WP_025247431.1 LysE family transporter -
  X874_RS03150 (X874_6210) - 653988..654461 (-) 474 WP_025247432.1 phosphatidylglycerophosphatase A -
  X874_RS03155 (X874_6220) thiL 654467..655447 (-) 981 WP_025247433.1 thiamine-phosphate kinase -
  X874_RS03160 (X874_6230) nusB 655510..655923 (-) 414 WP_025247434.1 transcription antitermination factor NusB -
  X874_RS03165 (X874_6240) comE 656003..657316 (-) 1314 WP_025247435.1 type IV pilus secretin PilQ Machinery gene
  X874_RS03170 (X874_6250) - 657325..657738 (-) 414 WP_025247436.1 hypothetical protein -
  X874_RS03175 - 657740..658273 (-) 534 WP_025247437.1 hypothetical protein -
  X874_RS03180 (X874_6260) - 658275..658796 (-) 522 WP_025216884.1 hypothetical protein -
  X874_RS03185 (X874_6270) - 658772..659473 (-) 702 WP_025247438.1 hypothetical protein -
  X874_RS03190 (X874_6280) - 659599..662151 (+) 2553 WP_025247439.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48367.43 Da        Isoelectric Point: 8.9392

>NTDB_id=115070 X874_RS03165 WP_025247435.1 656003..657316(-) (comE) [Mannheimia varigena USDA-ARS-USMARC-1312]
MFRIFLYLLLSSSLALADTISLAVKNAPTSLLLTYLAEETGKNIVLDDDIQTKSTLRLENKSINEIFKTLSKVNKLSFTQ
EADIVYISKKEEKSADLNSVSIAHLQNNIQNPPLVTSPKLITKTIKLNYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIIKDSAKSLQNIERLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGMFSRGTDHYKFGGRLEGNGLSNVANNLNVN
FPVANGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNTQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKVRGMKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=115070 X874_RS03165 WP_025247435.1 656003..657316(-) (comE) [Mannheimia varigena USDA-ARS-USMARC-1312]
ATGTTTAGAATTTTTCTGTATTTATTACTTAGTTCGTCATTAGCATTGGCTGACACTATTTCCCTTGCCGTAAAGAATGC
ACCGACTTCACTTCTGCTAACCTATTTAGCTGAAGAAACAGGTAAAAATATAGTATTAGATGATGATATTCAAACTAAAT
CCACCTTAAGATTAGAAAATAAATCCATTAATGAGATTTTTAAAACATTGAGCAAAGTAAATAAATTATCTTTTACTCAA
GAAGCAGACATTGTCTATATTAGTAAAAAAGAAGAAAAATCTGCTGATTTAAATTCAGTTTCAATTGCCCATTTGCAAAA
TAATATACAAAACCCACCGCTTGTAACTTCTCCAAAACTGATAACTAAAACTATAAAACTAAATTATGCTAAAGCCTCAG
AAGTGATTGAATCTTTAACTAAAGGCAGTGGCACATTTTTATCTGAAAATGGTTATATCCATTTTGACGAGCGAAGTAAT
AGCTTAATTATCAAAGATAGTGCGAAATCACTACAAAATATTGAGCGATTAGTCAAACAACTCGACCAACCCACCGAACA
AATTGCGATTGAAGCTCGCATTGTCACGATTAGTAGCGAACATTTGCAAGAATTAGGTGTGCGTTGGGGAATGTTTTCCA
GAGGAACTGATCACTATAAATTTGGTGGGCGATTGGAAGGAAATGGGTTAAGCAATGTAGCTAATAATCTCAATGTGAAT
TTCCCCGTTGCGAATGGTGCTTCAGCAGTTTTACAAGTAGCTTCCATTAATAGTCGAGTACTAGATTTAGAACTCAGTGC
ATTAGAACAGGAAAACAGTGTGGAAATCATTGCTAGCCCTCGTCTTTTAACCACTAATAAAAAACCTGCGAGTATCAAGC
AAGGCACAGAAATTCCTTATGTAATGTATAACACCAAATCAGAAGCAACCGATGTCGAATTTAAAGAAGCAGTGTTAGGA
TTGGAAGTTGTTCCTCATCTTTCTACTCAAAATCAAATTTTGTTGGATTTAATCGTCACTCAAAACTCACCGAATACACA
ATCTGGTAGCAGTGGTTTAATTACCATTGATAAGCAAGAACTTAATACACAGGTGTTTGCTAAACACGGCGAAACTATCG
TGCTAGGTGGTATTTTCCAACATTTAACCCAAAAAGGAGAAGATAAAGTACCTATTTTAGGCTCAATTCCGTTTATTAAA
CGATTATTTAGCCAAACAAGAGACAAAATCAGCAAGCGGGAACTTGTTATTTTTGTTACCCCTTACATTCTCCAATCAAA
CGAAAAAGTAAGGGGCATGAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)

Bacterial type II and III secretion system protein (Secretin)

(267-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.218

99.542

0.629
  comE Haemophilus influenzae Rd KW20

51.09

94.508

0.483
  comE Haemophilus influenzae 86-028NP

50.363

94.508

0.476
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.094

97.025

0.389
  pilQ Vibrio cholerae strain A1552

40.094

97.025

0.389
  pilQ Vibrio campbellii strain DS40M4

38.283

98.627

0.378

Multiple sequence alignment